Identification of microRNA associated with systemic lupus erythematosus in Korean

BACKGROUNDS: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by polyclonal B-cell activation and elevated production of pathogenic autoantibodies. MicroRNAs (miRNAs) are short, noncoding RNAs that regulate gene expression on the post translational level, which can be measured in the circulation and are emerging as novel biomarkers in various diseases. The measurement of circulating miRNAs provides important information concerning disease. Dysregulated expression profiles ofmiRNAs have been identified in the blood of patients with coronary artery disease, immune disease, and cancer. However,a systematic analysis of miRNAs in SLE patients has not yet been performed. In this study, we attempted to identify miNRAs associated with the susceptibility to SLE in Korean populations, and to elucidate their significance in clinical phenotypes of SLE. MATERIALS AND METHODS: Blood samples were collected from Korean SLE patients (n = 70)and normal controls (NC,n = 40)at the rheumatology clinic, Ajou University Hospital. The mean age of SLE patients was 33.5±7.3years and 90% were women. The mean age of NC was 33.1±' 6.3years and 90% were women. Peripheral blood mononuclear cells (PBMC) were isolated from blood samples of5 SLE patients and 8 normal controls. The miRNA microarray chip analysis identified miRNAs differentially expressed in SLE. For the microRNA PCR arrays, we isolated total RNA from plasma according to the manufacturer's instructions. The RNAs(n=10) were pooled in each sample group with an equal amount of RNAs. A miRNA expression profiling analysis was performed and compared between the SLE the normal controls groups. To verify the microRNA PCR array results, we performed the quantitive real-time PCR in samples from the patients with SLE (n=70), and the healthy controls (n=40). RESULTS: Four miRNAs were differentially expressed in plasma between SLE patients by miRNA PCR array. The plasma expression level of hsa-miR-17-5p, hsa-miR-19a-3p, hsa-miR-223-3p, andhsa-miR-30e-5p were up-regulated in the SLE group compared to the normal controls. The hsa-miR-223-3p and hsa-miR-30e-5p were significantly up-regulated in plasma from patients with SLE by quantitative real-time PCR. CONCLUSIONS: Our data suggest that plasma hsa-miR-223-3p and hsa-miR-30e-5p may be novel important promising biomarkers in the diagnosis of SLE. These novel and promising markers warrant validation in larger prospective studies.

Author dc:creator

• 김, 봉식

Contributors dc:contributor

• 서, 창희
• 대학원 의생명과학과
• 201124149

• 전신 홍반 루푸스
• 혈장
• miRNA
• 생물학적 표지자

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016405
000000016405

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/10855