Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Chip analysis"”.
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MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β
… binding site. Chromatin immunoprecipitation (ChIP) studies indicate that IFN-beta increases histone deacetylase (HDAC)-1 recruitment to the MMP-9 promoter and reduces histone H3 acetylation, in addition to reduced NF-kappaB recruitment. Accordingly, IFN-beta's repressive actions are abrogated …
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Galectin-3 Enhances The Malignant Melanoma Phenotype By Regulating Autotaxin
… and subjected to gene expression microarray analysis. We identified and validated the lysophospholipase D enzyme, autotaxin, a promoter of migration, invasion, and tumorigenesis, to be down regulated after silencing galectin-3. Silencing galectin-3 significantly reduced the promoter activity …
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Solvent compatible microfluidic platforms for solid form screening of pharmaceutical parent compounds
… by screening, crystallization, and subsequent analysis of various solid forms of a compound obtained. Currently the solid form screening process is performed by robotic platforms that require on the order of 0.5 g of material to screen 100 conditions. As a result, this screening effort is …
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Mcam/Muc18 Regulates Melanoma Progression By Modulating The Expression of Id-1
… A375SM and C8161, and conducted cDNA microarray analysis. We identified and validated that the transcriptional regulator, Inhibitor of DNA Binding-1 (Id-1), previously shown to function as an oncogene in several malignancies, was downregulated by 5.6-fold following MUC18 silencing. Additionally, …
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A Proteomic and Genomic Investigation Into Replication Fork Dynamics in Saccharomyces Cerevisiae
… wherein we used whole genome time-resolved ChIP-chip analysis of three integral members of the replication fork — the GINS complex, Polymerase ε, and Polymerase δ. Surprisingly, our data demonstrate that these proteins progress at highly uniform rates regardless of genomic location, …
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Mechanism of Chimeric Vaccine Mediated Immune Suppression of Human Dendritic Cells
… the cholera toxin B subunit (CTB-INS). Proteomic analysis of vaccine inoculated DCs revealed strong up-regulation of the tryptophan catabolic enzyme indoleamine 2, 3-dioxygenase (IDO1) shown to be directly linked to CTB-INS-induced DC activation and maturation. Treatment of vaccinated DCs with the …
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Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… the kidney. Chromatin immunoprecipitaion and DNA chip analysis (ChIP-chip) were performed to identify direct target genes of HNF-1Beta in the kidney. Phosphodiesterase 4C (PDE4C) was identified as an HNF-1Beta target gene. PDE4C belongs to the phosphodiesterase superfamily of enzymes that control …
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Identification of microRNA associated with systemic lupus erythematosus in Korean
… immune disease, and cancer. However,a systematic analysis of miRNAs in SLE patients has not yet been performed. In this study, we attempted to identify miNRAs associated with the susceptibility to SLE in Korean populations, and to elucidate their significance in clinical phenotypes of SLE. …
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Optimization and application of normally closed microvalves in microfluidics
… portability is highly desired, such as in on-chip analysis, crystallization screening, and in the study of chemical or biological processes over long durations of time. I utilized these optimized design considerations to fabricate a 4x6 multiplexed microfluidic platform capable of …
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Untersuchungen zu Regulatoren der Flavonoidbiosynthese in Arabidopsis thaliana : eine Studie zu neuen Funktionen bekannter Proteinklassen
… candidate target genes, resulting from ATH1 gene chip analysis. In this work three of these candidate target genes could be confirmed experimentally by using co-transfection assays. Besides R2R3-MYB transcription factors R3-MYB proteins are found in A. thaliana. An involvement in processes leading …
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Regulation of mammalian SINE transcription
… effect on B1 and B2 expression. RT-PCR analysis showed no increase in B1 and B2 RNA levels in MeCP2 null mice kidneys. ChIP analysis of Dnmt1n/n p53-/- embryonic fibroblasts, which have less than 5% of the normal DNA methylation level, showed significant reduction in MeCP2 and MBD2 …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… 12 weeks culture followed by standard nested PCR analysis. The samples consisted of 17 cultures (originated from 13 CD, 1 UC and 3 NIBD subjects), 24 buffy coat blood (originated from 7 CD, 2 UC, 11 NIBD and 4 healthy subjects) and 4 intestinal biopsies from 2 CD patients. Real time RT-PCR …
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Functional Analysis of the Tumor Metastasis Suppressor, NDRG1
… organs in animal models. In addition, our analysis of clinical cohorts data indicate that Wnt+/NDRG-/LRP+ signature has a strong predictable value for recurrence-free survival of cancer patients. Collectively, we have identified NDRG1 as a negative master regulator of Wnt signaling during …
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Complex Regulation of Pax6 Neuronal Progenitors By Rb Family Members During Corticogenesis
… we evaluated occupancy at the Rb promoter (ChIP analysis), proliferation, cell death (BrdU incorporation and TUNEL analysis) and changes in gene expression (RT-PCR) in wild type vs. mutant cortices from embryos lacking various Rb family members (Aim 2). Interestingly, we found evidence for …
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Control of Neuroendocrine Cell Physiology by a Single Transcription Factor, Drosophila Basic Helix Loop Helix Regulator DIMMED
… I use in vivo chromatin immunoprecipitation: ChIP) followed by measuring DIMM binding to putative DIMM enhancers by quantitative Polymerase Chain Reaction: qPCR). This work is described in Chapter 2. Secondly, I extend DIMM ChIP analysis to identify direct DIMM transcriptional targets on a …
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The Functions of Human Dna2 in Mitochondrial and Nuclear DNA Maintenance
… using micro-fluidic- assisted replication track analysis: maRTA). Surprisingly, we did not observe slowing of the replication fork upon hDna2 or FEN1 depletion suggesting that replication fork progression is insensitive to Okazaki fragment maturation. However, maRTA analysis revealed that origin …
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Trim24 Orchestrates Metabolic Reprogramming and Emt In Breast Cancer
… in several cancers; however, a detailed analysis of how TRIM24 promotes tumorigenesis with progression to metastasis has not been elucidated. Prior to the metastatic progression of a tumor, cancer cells undergo a transition from an epithelial to a mesenchymal phenotype; termed as …
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Gain-of-Function Mouse Models to Investigate Biological Roles of Prmt6
… clearly necessary for the coactivator function. ChIP analysis demonstrated that PRMT6 was recruited to the IL-6 promoter upon TNF-alpha stimulation. Moreover overexpression of PRMT6 caused RelA shuttling into the nucleus in our system, which could justify, at least in part, the mechanism …
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Induced Gene Expression In Bladder Cancer: Kindlin-2 And Metallothionein-1x
… genes were selected for further experimental analysis based on their common association with bladder cancer. Metallothionein isoforms 1 & 2 (MT I/II) are over-expressed in high-grade muscle-invasive bladder cancers, and in one study, MT-1X showed increased levels of expression compared to the …
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Power and performance optimization of negative capacitance transistor circuits
… is then extended to logic gate and full-chip designs. The characteristics of several logic gates are carefully studied to associate observed power-performance effects with root-cause parameters. Two full-chip benchmarks, a cell-dominated and a wire-dominated design are used for full-chip …
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