Covalent coupling of growth factors to collagen matrices: a novel development towards a tissue substitute with enhanced angiogenesis

Tissue engineering has been recognized in recent years as a viable solution for repair of tissue defects. Since engineered tissues (as vascular graft) do not have angiogenic capability by themselves, the introduction of Vascular Endothelial Growth Factor (VEGF) could be used to enhance angiogenesis into the prosthesis tissues. A major challenge in the development of a sustained-release of protein drugs is to achieve a high drug loading sufficient for a prolonged therapeutic effect. To overcome rapid diffusion and clearance from the implant site and to increase stability, VEGF was covalently bound to collagen sponges by means of the homobifunctional cross-linking agent bis-(succinimidyl succinate) polyethylene glycol (SS-PEG3400-SS), after coupling activity can then be compared with control experiments in which the VEGF was simply admixed. In order to prevent rapid degradation, collagen sponges used as protein drug delivery substrate were cross-linked by SS-PEG3400-SS. The biological behavior of collagen sponges as well as tissue and cell reactions after the treatment of SS-PEG3400-SS are investigated.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2001

Author dc:creator

• Chen, Jingsong

Contributors dc:contributor

• Pallua, Norbert

• info:eu-repo/classification/ddc/610
• Medizin

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

eng

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118835%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56748