{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:56748"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:56748","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Covalent coupling of growth factors to collagen matrices: a novel development towards a tissue substitute with enhanced angiogenesis","abstract":"Tissue engineering has been recognized in recent years as a viable solution for repair of tissue defects. Since engineered tissues (as vascular graft) do not have angiogenic capability by themselves, the introduction of Vascular Endothelial Growth Factor (VEGF) could be used to enhance angiogenesis into the prosthesis tissues. A major challenge in the development of a sustained-release of protein drugs is to achieve a high drug loading sufficient for a prolonged therapeutic effect. To overcome rapid diffusion and clearance from the implant site and to increase stability, VEGF was covalently bound to collagen sponges by means of the homobifunctional cross-linking agent bis-(succinimidyl succinate) polyethylene glycol (SS-PEG3400-SS), after coupling activity can then be compared with control experiments in which the VEGF was simply admixed. In order to prevent rapid degradation, collagen sponges used as protein drug delivery substrate were cross-linked by SS-PEG3400-SS. The biological behavior of collagen sponges as well as tissue and cell reactions after the treatment of SS-PEG3400-SS are investigated.","abstract_html":"Tissue engineering has been recognized in recent years as a viable solution for repair of tissue defects. Since engineered tissues (as vascular graft) do not have angiogenic capability by themselves, the introduction of Vascular Endothelial Growth Factor (VEGF) could be used to enhance angiogenesis into the prosthesis tissues. A major challenge in the development of a sustained-release of protein drugs is to achieve a high drug loading sufficient for a prolonged therapeutic effect. To overcome rapid diffusion and clearance from the implant site and to increase stability, VEGF was covalently bound to collagen sponges by means of the homobifunctional cross-linking agent bis-(succinimidyl succinate) polyethylene glycol (SS-PEG3400-SS), after coupling activity can then be compared with control experiments in which the VEGF was simply admixed. In order to prevent rapid degradation, collagen sponges used as protein drug delivery substrate were cross-linked by SS-PEG3400-SS. The biological behavior of collagen sponges as well as tissue and cell reactions after the treatment of SS-PEG3400-SS are investigated.","abstract_has_math":false,"creators":["Chen, Jingsong"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Pallua, Norbert"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001","date_published":"2001","updated_at":"2026-07-30T19:42:01Z","subjects":["info:eu-repo/classification/ddc/610","Medizin"],"languages":["eng"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118835%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118835%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118835%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/56748","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56748","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pallua, Norbert"]},{"key":"dc:creator","label":"Author","values":["Chen, Jingsong"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2001"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-1767"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/56748","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118835%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Tissue engineering has been recognized in recent years as a viable solution for repair of tissue defects. Since engineered tissues (as vascular graft) do not have angiogenic capability by themselves, the introduction of Vascular Endothelial Growth Factor (VEGF) could be used to enhance angiogenesis into the prosthesis tissues. A major challenge in the development of a sustained-release of protein drugs is to achieve a high drug loading sufficient for a prolonged therapeutic effect. To overcome rapid diffusion and clearance from the implant site and to increase stability, VEGF was covalently bound to collagen sponges by means of the homobifunctional cross-linking agent bis-(succinimidyl succinate) polyethylene glycol (SS-PEG3400-SS), after coupling activity can then be compared with control experiments in which the VEGF was simply admixed. In order to prevent rapid degradation, collagen sponges used as protein drug delivery substrate were cross-linked by SS-PEG3400-SS. The biological behavior of collagen sponges as well as tissue and cell reactions after the treatment of SS-PEG3400-SS are investigated."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 101 S. : Ill., graph. Darst. (2001). = Aachen, Techn. Hochsch., Diss., 2001"]},{"key":"dc:title","label":"Title","values":["Covalent coupling of growth factors to collagen matrices: a novel development towards a tissue substitute with enhanced angiogenesis"]}]}],"canonical_facts":{"dc:contributor":["Pallua, Norbert"],"dc:coverage":["DE"],"dc:creator":["Chen, Jingsong"],"dc:date":["2001"],"dc:description":["Tissue engineering has been recognized in recent years as a viable solution for repair of tissue defects. Since engineered tissues (as vascular graft) do not have angiogenic capability by themselves, the introduction of Vascular Endothelial Growth Factor (VEGF) could be used to enhance angiogenesis into the prosthesis tissues. A major challenge in the development of a sustained-release of protein drugs is to achieve a high drug loading sufficient for a prolonged therapeutic effect. To overcome rapid diffusion and clearance from the implant site and to increase stability, VEGF was covalently bound to collagen sponges by means of the homobifunctional cross-linking agent bis-(succinimidyl succinate) polyethylene glycol (SS-PEG3400-SS), after coupling activity can then be compared with control experiments in which the VEGF was simply admixed. In order to prevent rapid degradation, collagen sponges used as protein drug delivery substrate were cross-linked by SS-PEG3400-SS. 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