Publikationsserver der RWTH Aachen University
Nachweis von Antikörpern gegen Exophiala dermatitidis bei Patienten mit zystischer Fibrose
Abstract
dc:descriptionExophiala dermatitidis is a potentially pathogenic fungus frequently recovered from sputum of patients with cystic fibrosis (CF). The pathogenic role is virtually unknown, but CF patients infected with this fungus are at high risk of developing invasive disease. So far monitoring relies only on long-term fungal culture (4 weeks). Due to limitations of this procedure, the development of an additional easy-to-perform assay for detection of specific antibodies would facilitate monitoring. Therefore, an immunoassay was developed using commercially available precalibrated microaffinity columns (ABICAP TOOL, Biognosis, Jülich, Germany) containing ready-to-use activated solid phase matrix. By this means 108 sera from three groups (A: CF patients with and B: patients without E. dermatitidis recovered from sputum; C: healthy blood donors) were analyzed for prevailing IgG-, IgA- and IgE-antibodies using an antigen prepared from E. dermatitidis ex-type strain (CBS 207.35). In group A 83%, in group B 35% and in group C 6% of the test results for IgG, in group A 42%, in group B 24% and in group C 4% of the test results for IgA were above the upper limitting value. Thus, statistically significant differences between group A and C were revealed for IgG-, IgA and IgE-antibody production. In case of group A and B a significant difference was determined only for IgG-antibody production. In conclusion, the developed immunoassay proved to be well suited for serologic monitoring of colonized CF patients.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2001
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Conrad, Cordula
- Contributors dc:contributor
-
- Schweizer, Klaus
Subjects
dc:subject × 2Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger