Relevanz der Transmembran- und Intrazellulärregion von gp130, LIFR und OSMR für deren Liganden-induzierte Signaltransduktion

The family of IL-6-type cytokines requires three signal transducing receptor chains in order to transmit signals into cells: gp130, LIFR and OSMR. Here it could be demonstrated that the signal transducing capacities of LIFR and gp130 are highly redundant. The box1/2 region of the LIFR recruits the Janus kinases Jak1, Jak2 and Tyk2; the membrane distal tyrosine residues are sufficient for activation of the transcription factors STAT1 and STAT3. The OSMR in contrast recruits via its cytoplasmatic tyrosine residue 861 specifically the adapter protein Shc. Point mutation of this tyrosine residue to phenylalanine resulted in a reduced activation of the MAP kinases Erk1/2 as well as in a reduced induction of an a2-macroglobulin-promoter driven reporter gene. The box1/2 region of gp130 does - in contrast to the tyrosine residues - not operate as an independent, functional module, i.e. it cannot be transferred to a different position within the cytoplasmatic region of the receptor. Using point mutants it could be demonstrated that specific amino acids within the transmembrane region of gp130 are important for signal transduction. Insertions and deletions of amino acids in the transmembrane domain led to an altered receptor activity, demonstrating the importance of this region for signal transduction

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2000

Author dc:creator

• Hermanns, Heike Margarete

Contributors dc:contributor

• Heinrich, P. C.

• info:eu-repo/classification/ddc/570
• Biowissenschaften, Biologie

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118547%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56447