Reduktion der intraperitonealen Adhäsion humaner Magenkarzinomzellen in Nacktmäusen

Objective: Adhesion of tumour cells to mesothelial cells or extracellular matrix components is a pivotal step in developing peritoneal dissemination after gastric cancer. As phospholipids were found to reduce adhesion formation, especially at sites of peritoneal lesions(1) we assessed the inhibition of attachment of NUGC-4 gastric cancer cells by local treatment with phospholipids to the peritoneum in nude mice. Methods: In 90 female BALB/C nu/nu mice gastric cancer cells (1x10^6) were injected intraperitoneally suspended in either normal saline (controls), or phospholipid suspension 75mg/kg BW (PL75) or 150mg/kg BW (PL150). The treatment groups were subdivided into animals with defined peritoneal lesions and animals without lesions. After 30 days the extent of peritoneal carcinosis (mm², ml) and the Peritoneal Cancer Index were evaluated. Statistical analysis was performed with two factorial ANOVAs. The level of significance was adjusted according to Bonferroni (alpha = 0.00278). During a 90 days observation period the survival rate was determined using the Log-Rank-Test. Results: After 30 days the intraperitoneal tumour volume could be reduced by PL150 up to a value of 0.6 ml (SEM 0.16) and 0.48 ml (SEM 0.09) with peritoneal lesion compared to 0.9 ml (SEM 0.2) and 0.9 ml(SEM 0.1) in the control group (p = 0.04). The mean area of tumour adhesion amounted up to 145 mm² (SEM 17; p = 0.08) and 164 mm² (SEM 32.8; p = 0.049) with peritoneal lesions after treatment with PL150 (control: 216 mm² (SEM 28.5) and 245 mm² (SEM 29.3). The peritoneal cancer index was 16.4 (SEM 1.7) in the control group and 9 (SEM 1.68) with PL150 (p = 0.0002). In the subgroup with peritoneal lesions the respective values were: control 20.8 (SEM 0.85) and PL 150 14.3 (SEM 1.07; P = 0.0001). We found a prolonged survival rate after treatment with PL150. However, this effect was not significantly different to those of the control group. Treatment with PL75 had no significant influence. Conclusion: Phospholipids may be an efficacious and economic tool to reduce peritoneal tumour cell adhesion and consecutively the development of peritoneal carcinosis after resection of gastric cancer.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2005

Author dc:creator

• Zuber, Sebastian Alexis

Contributors dc:contributor

• Schumpelick, Volker

• info:eu-repo/classification/ddc/610
• Medizin
• Peritonealkarzinose
• Magenkarzinom
• Adhäsionsprophylaxe

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-114334%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A52092