Publikationsserver der RWTH Aachen University
Die Bedeutung von Zinkionen für mononucleäre Leukocyten
Abstract
dc:descriptionZinc is an essential trace element for the immune system. In this work, important influences of zinc on mononuclear cells were examined.For the first time, a direct stimulation of cells by zinc could be shown, which was dependent on zinc concentration and could not be induced by other cations. Even in physiological doses, zinc interacts significantly with intracellular processes. Cell-lines as well as primary cells express the zinc uptake transporters hZIP1, hZIP2 and hZIP3. Quantitative analyses showed, that hZIP3 is the main zinc transporter in monocytes, T-cells and PBMC, whereas hZIP1 is the highest expressed zinc transporter in B-cells. hZIP2 was only detectable at a very low level in all examined cells. All three transporters were upregulated in response to zinc deprivation. The assessment of transporter expression in cell lines and in primary cells showed a five- to tenfold expression in primary cells (PBMCs) in comparison with cell lines. Also, a decrease in transporter expression was detectable when primary cells were induced to proliferate by the superantigen SPEA or were immortalised by Epstein-Barr-Virus. A similar phenomenon could be observed in vivo, since hZIP transporter expression in PBMC of patients with chronic lymphatic leukaemia was significantly decreased when compared to the PBMC of patients with bronchial carcinoma. Additionally, proliferation assays demonstrated a central role of zinc in apoptosis.An examination of the interaction of zinc with intracellular signal transduction showed that zinc is able to inhibit IL-4 mediated proliferation of the T cell line CTLL-2. Contrary, zinc in combination with IL-4 acted synergistically on the B cell line B9 and induced a significantly higher proliferation rate than IL-4 alone. Both cell lines differ in their CD45 expression. Furthermore, zinc was able to abolish the inhibitory effect of an anti-CD45 antibody on the mixed lymphocyte culture (MLC).The mechanisms of direct and indirect zinc stimulation and the function of zinc uptake transporters that were examined in this work emphasize the importance of zinc for mononuclear cells in vitro and in vivo.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2006
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Ibs, Klaus-Helge
- Contributors dc:contributor
-
- Rink, Lothar
Subjects
dc:subject × 10Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger