Untersuchung molekularer Reaktionen nach zentralen und peripheren Nervenläsionen bei der Ratte unter besonderer Berücksichtigung von Neurodap1

This thesis describes the molecular reactions to regenerate nerve cells after lesion of the spinal cord in rats. Special emphasis is laid on the differences between the central and peripheral nervous systems, since the central system is not able to regenerate after axotomy. In particular, we wanted to investigate this process with the gene-fishing-method of Atlas cDNA Expression Arrays. We used the well-established experimental model of hemisection of the spinal cord at the level of C2. After the axotomy, we compared the characteristics in the expression of genes of the operated and non- operated sides in the example of the Red Nucleus. Among the up-regulated genes we found the protein Neurodap1, which is being described in the literature as down-regulated after axotomy in the peripheral nervous system. It is found in the big cells of the nervous system and is associated with the postsynaptic density region (PSD). We, therefore, investigated the characteristics of the expression of this gene after axotomy in the central and peripheral nervous systems with insitu-hybridization. Adjacent to the experimental model of C2 hemitranssection, we used in the peripheral nervous system the well-established experimental model of the facial nerve. Comparison of the operated and non- operated sides revealed differences in the expression of Neurodap1. To investigate the differences in time, we varied the time the animals had to regenerate between the axotomy and the euthanisation. We found that in the peripheral nervous system the down-regulation of Neurodap1 after axotomy is regressive with time. The insitu-hybridization for the central nervous system revealed a down-regulation of Neurodap1 as well, which is in contrast to the results from the Atlas cDNA Expression Array. Variation in time showed that the difference in expression is not time-dependent. On the first day after axotomy it was as high as after 8 weeks. This is a significant discrepancy between the peripheral and the central nervous system. The results of this thesis leave open the possibility to annihilate the differences in the ability of the two nervous systems to regenerate with suitable drugs.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2009

Author dc:creator

• Röth, Anjali Anna Joy

Contributors dc:contributor

• Brook, Gary

• info:eu-repo/classification/ddc/610
• Zentralnervensystem
• Peripheres Nervensystem
• Querschnittslähmung
• Regeneration
• Microarray
• In-situ-Hybridisierung
• Medizin
• Axotomie
• central nervous system
• peripheral nervous system
• axotomy
• spinal cord injury
• array

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113731%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A51440