Effekt einer transendokardialen kathetergestützten Applikation von Stromal cell derived factor 1[alpha] auf die linksventrikuläre Funktion im Herzinfarktmodell im Schwein

Aim of the study was to investigate the effect of catheter-based transendocardial delivery of stromal cell-derived factor 1 alpha on left ventricular function in a porcine model of myocardial infarction. In domestic pigs myocardial infarction was induced via microembolization of the distal left anterior descending artery. Catheter-based transendocardial injection of SDF-1alpha into the infarct and periinfarct myocardium was performed two weeks after myocardial infarction (n = 12). A control group underwent sham-intervention (n = 8). Two and seven weeks after myocardial infarction electromechanical mapping (EMM) of the left ventricle was performed to analyse left ventricular function (Ejection fraction, stroke volume, unipolar voltage, linear local shortening). Furthermore infarct size was determined by tetrazolium staining. Vessel density and myocardial remodelling were acquired by immunofluorescence staining. Immunofluorescence staining revealed significantly more vWF-positive vessels in SDF-1 alpha treated animals than in controls. Infarct size was similar in both groups. Ejection fraction and stroke volume decreased in SDF-1alpha animals and even increased in control animals. Linear local shortening, another parameter of ventricular function, did not change in controls but decreased significantly in SDF-1alpha treated animals. The catheter-based transendocardial delivery of stromal cell-derived factor 1 alpha in this porcine model of myocardial infarction increased periinfarct vessel density, but impaired myocardial function. The infarct size remained unchanged. Although SDF is a promising approach in stem cell recruitment clearly more research is needed to exploit the capability of homingfactors in myocardial regeneration.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2009

Author dc:creator

• Rammos, Christos

Contributors dc:contributor

• Koch, Karl-Christian

• info:eu-repo/classification/ddc/610
• Herzinfarkt
• Stammzelle
• Stammzellenfaktor
• Angiogenese
• Medizin
• Homingfaktor
• SDF
• endokardiales Mapping
• Noga
• electromechanical mapping
• stem cell homing

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-113335%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50808