Auswirkung von körperlicher Belastung auf die Konzentration von 3-Nitrotyrosin im Atemkondensat

Due to its function, the lung is more likely exposed to environmental influences than other organs of the human body. Frequently the occupational medicine and health care have to deal with complaints due to pulmonary reasons, which cannot be detected by established diagnostic methods (i.e. spirometry), as those methods do only examine existing structural and functional pulmonary damages. The analysis of breath condensate is a new non-invasive method to examine processes in the air passages. The breath condensate contains non-gaseous compounds of exhaled breath, which reflects the composition of the bronchoalveolar extracellular lining fluid. The marker 3-nitrotyrosine, which can be detected in breath condensate, helps to examine processes of oxidative stress in the lower airways. At the present time the analysis of breath condensate is not established in the diagnostic routine. This study claims to improve this matter. 20 probands (aged 20-35 years, equally male and female genders) were examined due to the changes of 3-nitrotyrosine concentration in exhaled breath condensate (EBC) caused by physical exercise testing. They were separated in 10 atopic and 10 non-atopic probands. Each group contained 5 smokers and 5 non-smokers. Eventually we got four groups of probands: 1. smoker/atopics; 2. smoker/ non-atopics; 3. non-smoker/atopics; 4. non-smoker/non-atopics.A standardized questionnaire documented the current health status including the atopic status and smoking habitsof each proband. After a physical examination and lung function testing (spirometry respectively bodyplethysmography), the first frequency-controlled breath condensate donation took place (point of time T0), which lasted 15 minutes. Following a cardiopulmonary exercise testing took place until the proband reached his anaerobic threshold. The second breath condensate donation (length of time 15 minutes; point of time T1) was executed 15 minutes after reaching the anaerobic threshold, which was verified by an interpretation of capillary blood gas testing. The third breath condensate donation (point of time T2) was performed 90 minutes after reaching the anaerobic threshold. The nitrotyrosine concentration was determined by LC-MS/MS after SPE-accumulation. The mean concentration of nitrotyrosine (in pg/ml) before and after exercise testing were 50,83 pg/ml (SD: ± 31,27), 51,94 pg/ml (SD: ± 27,81), 25,57 pg/ml (SD: ± 20,33). The median nitrotyrosine concentration (in pg/ml) before and after exercise testing were 39,1 pg/ml (IQR: 47,55), 50,1 pg/ml (IQR:45,15), 18,8 pg/ml (IQR: 24,05). CONCLUSION: Physical exercise shows no significant effect on the 3-nitrotyrosine concentration in exhaled breath condensate (EBC).

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2009

Author dc:creator

• Gerritz, Nicola

Contributors dc:contributor

• Kraus, Thomas

• info:eu-repo/classification/ddc/610
• Ergometrie
• Fahrradergometer
• Lungenfunktion
• Lungenfunktionsprüfung
• Oxidativer Stress
• Lunge
• Lungenvolumen
• Deutsche Gesellschaft für Arbeitsmedizin
• Arbeitsmedizin
• Biomarker
• Chromatographie
• Atopie
• Rauchen
• Medizin
• Atemkondensat
• Nitrotyrosin
• Spirometrie
• Bodyplethysmographie
• exhaled breath condensate
• physical exercise
• nitrotyrosine
• oxidative stress
• EBC

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112830%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50279