Zyklusabhängige Expression und Lokalisation der Isoform C des Progesteronrezeptors im humanen Endometrium im Vergleich mit den Isoformen B und A

The progesterone receptor (PR) is expressed in three major isoforms which mediate the effect of progesterone. These isoforms are translated from distinct mRNAs and differ in their molecular weight since PR-A and PR-C are truncated at the N-terminus. PR-B is the largest isoform with a molecular weight of 114 kDa, followed by PR-A with a molecular weight of 94 kDa and PR-C with a molecular weight of 60 kDa. In this study, the expression of these isoforms in human endometrial tissue was investigated by Western Blot analysis. In comparative experiments different antibodies against the progesterone receptor have been tested and it could clearly be shown that the isoform C can only be detected by antibodies which react with the C-terminus of the progesterone receptor protein. All three isoforms have been detected in human breast cancer cells (T47D) and were identified in this study for the first time in human endometrium as well. In total, 74 samples of uterine tissue from women subjected to hysterectomy because of benign uterine diseases (uterine leiomyoma, uterine prolapse) were investigated. For the study, cytoplasmic and nuclear extracts were prepared according to the protocol of the first description of the PR isoform C. In addition, one third of the samples were separated in epithelial cells and fibroblasts to assess the expression of the isoforms in the different types of cells. The expression of all three isoforms was shown to be dependent on the human menstrual cycle. Only with detailed knowledge of the regulation of the healthy endometrium pathological findings can be assessed. Thus, this study provides a basis for evaluating the aberrant distribution of isoforms in gynaecological diseases such as endometrial carcinoma as well as endometriosis.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2008

Author dc:creator

• Hendricks, Kira

Contributors dc:contributor

• Claßen-Linke, Irmgard

• info:eu-repo/classification/ddc/610
• Gebärmutterschleimhaut
• Progesteronrezeptor
• Medizin
• Endometrial tissue
• Progesterone receptor

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-112678%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A50121