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Showing 1 to 20 of 206 for “"yeast two"”.

  1. Yeast two-hybrid analysis of skeletal muscle triad junction proteins

    Proenza, Catherine, author, Beam, Kurt G., advisorColorado State University. Libraries1999

    … triad junctions. In this dissertation, the yeast two-hybrid system was used to search for interactions among known and novel proteins of triad junctions. In direct interaction tests, no strong interaction was detected between the dihydropyridine receptor (DHPR) II-III loop and the skeletal …

    colostate Repository record

  2. Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach

    Farzadfar, RahinUniversity of Ottawa (Canada)2013

    … repair) or apoptosis. Artemis and PARP-1 are two proteins with significant implications in the various branches within the DNA damage response network. A series of yeast two hybrid screening (Y2H) experiments were conducted using both classical and interacting mating Y2H systems to find …

    ottawa-retro Repository record

  3. Suche nach Interaktionspartnern mit dem ATP-abhängigen Kaliumkanal der Niere, ROMK, durch "Yeast-Two-Hybrid-Screening"

    Roos, Marcel PhilippUniversität Würzburg

    … Proteinen erhoffen. Nach Durch-führung dreier „Yeast-Two-Hybrid“-Screens zur Identifizierung möglicher Interakt-ionspartner von ROMK kamen 17, von ihrer Funktion schon bekannte, aussichtsreiche Proteine, in die enge Auswahl. Nach weiterer Charakterisierung und Autoaktivierungs-tests blieben 13 …

    wurz-thes Repository record

  4. Charakterisierung des Chaperons Mdg1 unter Berücksichtigung der subzellulären Lokalisation und Identifizierung der Interaktionspartner mit Hilfe des Yeast-Two-Hybrid-Systems

    Müller, Tina

    … werden. <br>Für Interaktionsstudien wurde das Yeast Two Hybrid-System eingesetzt. Neben der Interaktion mit dem den Zellzyklus kontrollierenden E2FBP1 und der Kolokalisation im Kern nach Hitzeschock konnte eine vermutete Mdg1-Mdg1-Interaktion im Hefesystem bestätigt werden. Diese Informationen …

    freiburg-diss Repository record

  5. Development of a split-ubiquitin based, cytosolic yeast two-hybrid system and its application to the structure-function analysis of scUri1p

    Möckli, NatalieETH2007

    ethz Repository record

  6. Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2

    Kumar, Deepak2005

    … that partake in a variety of DNA repair. Two of the most common types of damage are double-strand breaks (DSBs) and interstrand crosslinks. A single DSB or crosslink can potentially kill a cell if it is not repaired~ In human and other vertebrate cells, DSBs are repaired by two different …

    u-pacific Repository record

  7. Identification of Proteins Interacting with the Poly C Binding Protein 1 in the Central Nervous System Using Yeast Two Hybrid Screening System

    Odiase, Joanne Eghenayahiore2014

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    shu-thes Repository record

  8. A yeast two-hybrid screen in Arabidopsis thaliana suggests FORKED1 forms homodimers and the ARFA1 family has a role in cotyledon vein patterning

    Erickson, Jessica, University of Lethbridge. Faculty of Arts and Science2011

    lethbridge

  9. Untersuchung von PRV-1 auf Protein-Protein-Interaktions-partner im Yeast-Two-Hybrid Assay und weitere Aufklärung der 5'cDNA-Sequenz einer potentiellen Tyrosinkinase

    Buchkremer, Stefan

    … physiologischen Bedingungen wurde hierzu eine Yeast-two-hybrid-Screen verwendet. Dabei wurde PRV-1 ohne die natürliche Leadersequenz in-frame in den Bait-Vektor pGBKT7 kloniert. Anschließend wurde das Bait-Konstrukt in den Hefestamm Sacharomyces cerevisiae AH109 transformiert. Die korrekte …

    freiburg-diss Repository record

  10. Interaction studies of the cellular prion protein

    Hundt, ChristophLudwig-Maximilians-Universität

    … under native conditions. This was proven in the yeast two-hybrid system followed by the identification of two interaction domains. The influence of mutations and polymorphisms within the prion gene was investigated in the yeast two-hybrid system. This method is also an useful tool for the …

    lmu-germany Repository record

  11. Identifizierung neuer Interaktionsproteine des humanen Septins SEPT4 (hCDCrel-2) in fetalem Gehirn

    Horn, Julia Catherina

    … von SEPT4 in fetalem Gehirn mit dem Yeast Two-Hybrid System charakterisiert: SEPT8, SEPT11 und die Proteine Filamin B, MURR1 und die Ubiquitin-spezifische Protease 31. Es wurde in dieser Arbeit gezeigt, dass auch der N-Terminus von SEPT4 in Interaktionen mit Septinen und anderen …

    freiburg-diss Repository record

  12. Molekulare Analyse der Yersinien-Wirtszell Interaktion

    Abahji, Thomas N.Ludwig-Maximilians-Universität

    … YopO zu identifizieren. Als Methode diente das Yeast Two- Hybrid System. Es stellte sich heraus, dass YopM für diese Methode aufgrund seiner transskriptionsaktivierenden Eigenschaft nicht verwendbar ist. Dies und die Beobachtung, dass YopM in den Zellkern lokalisiert wird (77) deuten darauf hin, …

    lmu-germany Repository record

  13. The Use of C. Elegans to Identify Mutations That Confer Drug Resistance, Using Hemiasterlin as an Example

    Cardenas, Eduardo Cardenas2012

    … hopes of gaining insight into HAR-2 function, a yeast-two-hybrid screen of a C. elegans cDNA library was performed using HAR-2 cDNA as the bait. The yeast-two-hybrid experiment identified Rps-1 most frequently as an interactor with HAR-2. Rps-1 is the homolog to the human S3A small ribosomal …

    utswmed Repository record

  14. FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT

    Qu, EnpingTemple University. Libraries2015

    … that involves in intra-Golgi transport. In a yeast two hybrid screen by Bruno Goud group in 1995, they used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, Rab11 and Sorting …

    temple Repository record

  15. Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators

    McDermott, Hyoeun Ahn

    Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) …

    mo-state Repository record

  16. Das Sap47-Gen aus Drosophila melanogaster : Gezielte Mutagenisierung und Suche nach Interaktionspartnern

    Funk, NataljaUniversität Würzburg

    … das SAP47 Protein zu identifizieren wurden ein Yeast-Two-Hybrid System und das "CytoTrap" Verfahren eingesetzt.

    wurz-thes Repository record

  17. Identification of motifs in the tribbles kinase required for substrate interactions

    Pence, Laramie EliseUniversity of Missouri--Kansas City2013

    … degradation that ensues from this interaction, a yeast two hybrid system was established to measure interaction strength by growth on selective media and in a quantitative reporter gene assay. Using site-directed mutagenesis, Trbl mutants were constructed based on these conserved motifs and used …

    umkc Repository record

  18. Characterization Of A Novel Interactor/substrate For The Pro-apoptotic Serine Protease Omi/htra2

    Stratico, Valerie Anne

    … p38 stress-activated kinase, using a modified yeast two-hybrid system. Omi localizes in the mitochondria and in response to apoptotic stimuli the mature form of this protein translocates to the cytoplasm. In the cytoplasm Omi participates in both the caspase-dependent as well as …

    ucf

  19. Characterization of ESl47, a salt stress regulated protein kinase from Lophopyrum elongatum

    Elias, Demetra2001

    … in salt tolerance and salt stress response. The yeast two-hybrid system was used to screen a cDNA Arabidopsis library and identify proteins that interact with the ESI47 kinase. Six partial DNA sequences encoding a 33 kDa oxygen-evolving enhancer precursor protein (OEE1) from the photosystem II …

    concordia Repository record

  20. Identification and Characterization of Pat-6/actopaxin in Caenorhabditis Elegans Body Wall Muscle

    Lin, XinyiUniversity of Illinois at Urbana-Champaign2015

    … kinase domain. K10B2.5 binds PAT-6/actopaxin in yeast two-hybrid assays, and also colocalizes with PAT-6/actopaxin to muscle attachment sites.

    uiuc Repository record

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