Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"wortmannin"”.
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PI3K/Akt activation is critical for early hepatic regeneration after partial hepatectomy
… these studies, I used the potent PI3K inhibitor, wortmannin, and small-interfering RNA (siRNA) targeting the p85ƒÑ and p110ƒÑ subunits to determine if total or selective PI3K inhibition would abrogate the proliferative response of the liver following resection. After partial hepatectomy in mice, …
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Divergent Roles of PI3K and Akt in Rapamycin-induced Cardioprotection against Ischemia-Reperfusion Injury
… rapamycin), or rapamycin (0.25mg/kg, i.p.) plus wortmannin (WTN, 15µg/kg, i.p.),an inhibitor of phosphatidylinositol 3-kinase, or wortmannin alone (15µg/kg, i.p.). After 30 min of stabilization, the hearts were subjected to 20 minutes of global ischemia and 30 minutes of reperfusion in …
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SYNTHESIS AND SCREENING OF PI3K INHIBITOR PRODRUGS FOR TREATMENT OF PROSTATE CANCER
… have been identified as PI3K inhibitors, e.g., wortmannin, LY294002, and, ZSTK474, but all have low selectivity. We have addressed the specificity of current PI3K inhibitors by developing PSA (prostate-specific antigen)-activated analogues of known PI3K inhibitors LY294002 and ZSTK474.
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Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
… and blocked by PI-kinase inhibitors, wortmannin and adenosine, the dependency is not neutralized by the PI(3)-kinase inhibitor LY294002, PI(4,5)P2 ligands, such as neomycin, a PI-transfer protein that can remove PI from membranes, and PI(4,5)P2, PI(3)P and PI(4)P antibodies etc. In …
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Effects of Zinc on the Insulin Receptor and Insulin Signal Transduction Pathway
… by zinc or insulin. The PI 3-kinase inhibitor, wortmannin decreased basal glucose transport and blocked zinc-stimulated glucose transport. H7, an inhibitor of PKC, did not reduce basal glucose transport but decreased zinc-induced glucose transport in a dose-dependent manner. Zinc increased Tyr …
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Chemoprevention of Lung Carcinogenesis: Aerosol Administration and Deposition in the Mouse Lung
… inhibitors: gefitinib, erlotinib, lapatinib and wortmannin), and four natural agents: resveratrol, caffeine, anthocyanins, and protocatechuic acid), were considered as examples of single agents. Gefitinib and erlotinib were delivered in an aerosol form to reduce the cutaneous side effects. …
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Insulin and TSH signal transduction pathways in human preadipocytes.
… and have shown it activates PKB and p70 S6K in a wortmannin-sensitive manner. A model depicting the convergence of insulin and TSH signaling in human preadipocytes is proposed.
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Simulated impact trauma and osteoarthritis: the role of cell volume and mechanotransduction
… the phosphoinositide 3-kinases (PI3K) inhibitor wortmannin were used to determine its mechanism of action. Wortmannin alone increased cell death and inhibited REV5901 chondroprotective effects with no alteration in cell volume compared to REV5901 alone. Whilst uridine reduced cell volume with 20 …
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Die Hemmung der PI3K verstärkt die Kontraktilität in alpha1-adrenerg stimuliertem Myokard
… wurden mit dem PI3K Hemmstoff Wortmannin inkubiert (WM, 0.1 µ Mol/L)). Die Stimulation mit dem alpha1 Agonisten Phenylephrin (PE, 10 µ Mol/L) veranlasste eine signifikant stärkere Zunahme der Kontraktilität in WMbehandelten Herzmuskelzellen im Vergleich zur Kontrollgruppe. …
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Insulin Receptor Substrate-1 Serine Phosphorylation by a Novel Phosphatidylinositol-3'-Kinase-Associated Serine Kinase Regulates Insulin and Interferon Receptor Signaling
… PI 3-kinase is shown to associate with a wortmannin insensitive 76 kDa serine phosphoprotein (pp76) distinct from the p85 subunit of PI 3-kinase. pp76 is phosphorylated by an okadaic acid sensitive, PI 3-kinase associated serine kinase (PAS kinase) with biochemical properties that …
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Effects of naringenin on glucose uptake in L6 skeletal muscle cells
… Akt phosphorylation but inhibition of PI3K by wortmannin partially repressed the naringenin-induced glucose uptake. We also examined naringenin's effect on AMP-activated protein kinase (AMPK), a molecule that is involved in mediating glucose uptake by a variety of stimuli. Naringenin stimulated …
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PIP2 Modulation of Afterhyperpolarizations in Magnocellular Supraoptic Neurons
… OT and VP neurons. PIP2 depletion <em>via </em>wortmannin in the cells abolishes the mAHP and sAHP of OT but not VP neurons. This demonstrates OT neurons require PIP2 to activate an AHP while VP neurons do not. Interestingly, increasing PIP2 within the cells has little effect on OT neurons while …
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Mechanismen der Urocortin-II-induzierten Stimulation der NO-Produktion in isolierten Kaninchen-Ventrikelmyozyten
… der Phosphatidylinositol 3-Kinase (PI3K), Wortmannin (0,3 µM) und LY294002 (10µM) nahezu vollständig aufgehoben. Konfokale Bildgebung der zellulären NO-Produktion und der fraktionellen Verkürzung zeigen einen Anstieg des [NO]i und der fraktionellen Verkürzung mit einem ähnlichen …
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Mechanistic Studies and Inhibition of N-hydroxylating Monooxygenases
… two compounds were identified. These were, wortmannin a steroid metabolite and ebselen a benzoselenazole as SidA inhibitors with IC50 values of 369 µM and 11 µM respectively. A second part of this works investigates the hydroxamate formation of the siderophore albachelin in Amycolatoposis …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… and presence of the PI 3-kinase inhibitor wortmannin. The integration of these techniques provides a ratio-metric readout, allowing authentically 3- phosphoinositide (3-PI) responsive components to be distinguished from the co-purifying background proteins. The identification of tandem …
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Activity-Based Protein Profiling Reveals Changes to the Regulation of Enzymatic Activity by the Hepatitis C Virus
… by the hepatitis C virus (HCV). The novel probe wortmannin-yne is described and is shown to be able to report on the activity multiple kinases, including MAPK1, whose activity is dysregulated during HCV replication. Novel probes designed to target a smaller selection of kinases, …
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α-Lipoic Acid Attenuates Ischemia Reperfusion Injury of the Rat Liver
… Akt activation by simultaneous application of wortmannin, a selective PI-3 kinase inhibitor, abrogated the LA preconditioning effect, showing a causal involvement of Akt in LA-mediated protection from IRI. Therefore, Akt activation can be regarded as a new protective mechanism in hepatic IRI. …
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Identifying a role for Ciz1 in the DNA damage response
… This phosphorylation is inhibited by both wortmannin and LY294002, implicating DNA-PK as the responsible kinase. More traditional methods including immunofluorescence and RT-PCR were used to characterise the effects of DDR signalling upon endogenous and overexpressed Ciz1. The resulting …
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Differential Regulation of Phosphatidylinositol 4-Phosphate 5 Kinase Beta during Hypertonic and Oxidative Stress
… due to activation of a PI4K. Using RNAi and wortmannin to selectively inhibit the activity of different PI4K isoforms, I sought to identify the PI4K isoform that is responsible for this increase.
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Regulation der myokardialen Glukoseaufnahme : Mechanismen bei alpha-adrenerger Stimulation und die Rolle der AMP-aktivierten Proteinkinase bei Ischämie
… wurden diese Experimente mit dem PI3-K-Inhibitor Wortmannin (WM) wiederholt. Zur Überprüfung der Rolle der AMPK bei der Ischämie wurden isolierte Herzen von AMPK-KD- und Wildtyp (WT)-Mäusen über die Aorta mit KH-Puffer perfundiert und einer 30-minütigen Low-flow-Ischämie mit Reperfusion …
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