Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 53 for “"whole-cell patch clamp"”.
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Investigating mechanisms of biophysical diversity between phasic and tonic motor neurons
… To address this question, we combined in vivo whole-cell patch-clamp physiology with biophysical modeling. First, we characterized biophysical diversity between 1s and 1b MNs. To explore molecular mechanisms underlying such diversity, single-neuron PatchSeq RNA profiling experiments were …
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Investigating Mechanisms In Nociceptors Driving Ongoing Activity and Ongoing Pain
… to bridge the gap between RMP and AP threshold. Cellular signaling pathways that increase DSF amplitude and promote ongoing activity acutely in nociceptors were unknown for any neuromodulator, as were the neurophysiological alterations that underly ongoing activity in nociceptors after deep …
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Investigating cestode modulation of host neuronal excitability and cell-type-specific gene expression in an in vitro mouse model of neurocysticercosis
… and used singlenucleus RNA sequencing and whole-cell patch-clamp electrophysiology to investigate potential links between inflammation and network excitability at a transcriptomic and electrophysiological level. In the first part of the thesis, I found that while LPS significantly affected …
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Signal transformation at the input and output of the Drosophila visual system
… I focused on characterizing this current using whole-cell patch clamp in wild type and mutants, and using antibodies for Shal. My results from whole-cell patch clamp indicated that IKf in adult R1-6 cells are not Shal, from their voltage dependence and insensitivity to a Kv4 blocker. This calls …
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Elf magnetic field influence on ION Channels studied by Patch Clamp Technique: exposure set up and "Whole Cell" measurements on Potassium currents
… magnetic fields on potassium currents in neural cell lines ( Neuroblastoma SK-N-BE ), using the whole-cell Patch Clamp technique. Such technique is a sophisticated tool capable to investigate the electrophysiological activity at a single cell, and even at single channel level. The total potassium …
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Descending Control of Limb Movements in Drosophila melanogaster
… animal relatively intact. Lastly we use in-vivo whole cell patch clamp electrophysiology to characterize the role of individual DNs in response to specific sensory stimuli and in relationship to movement. We find that there are approximately 1100 DNs in Drosophila that are distributed across six …
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Cortical Pyramidal Neuron Subtype Classification and Visual Response Diversity
… neurons into the collective cortex suggests that cellular individuality may represent a smaller component of computational role in the context of the larger network. This possibility raises an important question: is the computational function of a neuron determined by its individual type or by its …
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Die Rolle der Kalzium-Calmodulin-abhängigen-Proteinkinase II δc (CaMKIIδc) bei der Radikal-vermittelten Zytotoxizität in isolierten ventrikulären Kaninchenmyozyten
… der späte Na+ - Strom mit Hilfe der Whole-cell-Patch-Clamp-Technik untersucht, ebenso wie der intrazelluläre Na+- und Ca2+ - Stoffwechsel mittels Epi.uoreszenzmikroskopie. Zum Test der Gegenhypothese wurden die Inhibitoren KN-93 und Ranolazin eingesetzt. Schließlich konnte gezeigt …
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TRPV1 ist ein polymodaler Rezeptor von nozizeptiven Spinalganglienzellen
In der vorliegenden Arbeit wurde mittels der Whole-Cell Patch-Clamp Methode sensible Neurone von transgenen Mäusen untersucht, bei denen das Gen für TRPV1 (transient receptor potential V1) deletiert wurde. Das Ergebniss wurde mit den Daten von Wildtyp Mäusen verglichen. TRPV1 (früher VR1; vanilloid …
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Hormone Regulation of NPY Neuron Activity in the Arcuate Nucleus of the Hypothalamus
… receptors present on NPY neurons, whole-cell patch clamp electrophysiology was performed on identified NPY neurons throughout the rostral-caudal extent of the arcuate nucleus of the hypothalamus. Leptin and insulin acutely hyperpolarized NPY neurons, and these effects were dependent …
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FEAR EXTINCTION AND INFRALIMBIC CORTEX IMPAIRMENT AFTER MILD TRAUMATIC BRAIN INJURY
… behavior, local field potential recordings, and whole-cell patch-clamp techniques to investigate how mild TBI affects IL network activity and excitability in a mouse model of TBI. Our results demonstrate that mild lateral fluid percussion injury (LFPI) significantly impairs fear extinction …
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Sensory Transduction in Enteroendocrine Cells
… of the gut epithelium where enteroendocrine cells act as sensor cells to secrete hormones in response to the luminal contents. This thesis focuses on voltage gated ion channels and the transient receptor potential (TRP) superfamily in I cells and enterochromaffin cells using the model tumour …
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Postnatal development of the somatosensory cortex in a rat model of Fragile X Syndrome
… an investigation of interneuron density. Using whole-cell patch clamp recordings from layer 4 of S1, I found that Fmr1-/y stellate cells (SCs) are hyperexcitable during early development, in agreement with previous research. Thanks to the high temporal resolution of these recordings, I have …
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Modulation of the sensitivity of nociceptive neurones by cannabinoids and SNSR
… was then employed to elucidate the intracellular pathway involved in this sensitisation, and protein kinase C (PKC) was found to play a significant role. The specific PKC-isoform involved, however, was not able to be elucidated using immunocytochemistry. Whole cell patch clamp studies …
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Investigation of Accessory Olfactory Interneuron Physiology and Function After Experience-Dependent Plasticity
… the accessory olfactory bulb (AOB), exhibits cellular and network plasticity in male and female mice after social experience. In the AOB, interneurons called internal granule cells (IGCs) express the plasticity-associated immediate-early gene Arc following intermale aggression or mating. Here, …
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Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… of Nav channels as potently as the intracellular fibroblast growth factor 14 (FGF14). FGF14 is a biologically relevant accessory protein of the neuronal Nav channel complex controlling gating, stability, and trafficking of native Nav channels. Through a monomeric interaction with the …
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PIEZO1 Channel-Mediated Mechanotransduction as a Driver of Cardiac Remodelling
… remains unclear. Fibroblasts are major effector cells of fibrotic remodelling. Still, the precise mechanism by which fibroblasts decode biomechanical cues in settings such as pressure overload has not been fully resolved. This thesis investigates the Ca2+-permeable mechanosensitive ion channel …
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Modelling neuroimmune interactions using organotypic slice cultures
… or in vitro culturing with dissociated neural cells. Both models have yielded crucial insights concerning CNS-TB but each have limitations e.g. lack of access to the brain during infection in vivo and absence of the 3D organizational tissue structure in vitro. This study investigated the effect …
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Voltage gated calcium channels – molecular targets for trigeminal neuralgia
… biophysical properties of these mutations using whole-cell patch-clamp recordings in tsA-201 cells. Second, we investigated the role of CaV3.2 channels in an animal model of trigeminal neuropathic pain. Finally, we explored the molecular mechanism of a selective T-type calcium channel blocker. …
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