Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"type three secretion system"”.
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Regulation of the Salmonella Pathogenicity Island 1 Type Three Secretion System
… enterica serovar Typhimurium is mediated by a Type Three Secretion System (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The SPI1 TTSS injects effector proteins into the cytosol of host cells where they promote actin rearrangement and engulfment of the bacteria. HilC, HilD, and …
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Tagging of the type three secretion system basal apparatus of Enterohaemorrhagic Escherichia coli 0157:H7
… Escherichia coli (EHEC) O157:H7 possesses a type III secretion system (T3SS). The T3SS translocates effector proteins into host cells during infection and consists of a basal apparatus that spans the inner and outer membranes and a needle and filament complex. The basal apparatus is composed …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
… humans. This gram-negative pathogen utilizes the Type Three Secretion System to transport effector proteins into the host cell. Several proteins are essential for the transportation of these effectors, they are the secretion needle subunit, AscF and pore forming translocators, AopB and AopD. Prior …
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The Chlamydia Trachomatis Protein Interaction Network: Insights into the Unique Composition of the Type Three Secretion System
… of genetic tools, we developed a yeast model system to identify and characterize chlamydial proteins involved in virulence mechanisms. In this study we describe the identification of twenty-eight proteins potentially involved in modulating host cellular functions and the secretion of virulence …
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The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… via the Salmonella Pathogenicity Island I (SPI1) type III secretion system (T3SS) to induce inflammatory diarrhea and bacterial uptake into intestinal epithelial cells. The master SPI1 regulatory gene hilA is controlled directly by three AraC-like regulators: HilD, HilC and RtsA. A periplasmic …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… injecting toxins into a host cell, called the type three secretion system (T3SS). The secretion machinery itself is highly complex and, together with the specific secreted factors, requires expression of more than 30 genes. Due to the high energy cost of its synthesis to the organism this …
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Analysis Of Yscf And Associated Homologs And The Presence Of An Inflammatory Molecular Pattern
… pathogen, Yersinia pestis, expresses a type three secretion system to accomplish its virulence. The YscF needle protein provides a conduit of effectors from bacterium to eukaryote and is exposed to the host immune system. This thesis explores YscF and its associated needle homologs and …
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Rational development of novel small molecule leads against the transcriptional activator protein ExsA of Pseudomonas aeruginosa
… environments, including water distribution systems and hospitals. This prevalence is concerning due to P. aeruginosa’s status as an opportunistic pathogen, and the latter particularly so because it is a common cause of nosocomial infections. Burn victims and cystic fibrosis patients are …
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Small molecule inhibitors against enteric bacterial glycosyltransferases
… Pathogenic bacteria evolved to develop various secretion systems to deliver virulence proteins termed ‘effectors’. These virulence factors promote the invasion of pathogens as well as evasion of host innate immune response. Type III secretion system (T3SS) is one of the most complex secretion …
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The impact of a single nucleotide polymorphism in fusA1 on biofilm formation and virulence in Pseudomonas aeruginosa
… of a major virulence determinant, the type three secretion system, typically characteristic of cells comprising an acute infection. Proteomic and transcriptomic profiling of the mutant strain provided an insight into the genetic basis behind these phenotypes, identifying the …
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Coordinated regulation of the flagellar, Salmonella pathogenicity island 1, and the type I fimbriae gene networks in Salmonella typhimurium
… In addition, there is cross-talk between these three systems which serves to dynamically control the timing of activation and de-activation of these networks. Collectively, we demonstrate that cells dynamically process information in genetic networks which ensures that the encoded products are …
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The Characterization of the Fucose Sensing Kinase (FUSK) and the Fucose Sensing Response Regulator (FUSR) and Their Role in Virulence Regulation in Enterohemorrhagic Escherichia Coli O157:H7
… (LEE) that encodes for a molecular syringe, a type three secretion system (T3SS), which translocates effectors to the host cell. Expression of the LEE is controlled by the AI-3/Epi/NE interkingdom signaling cascade. The two-component systems QseBC and QseEF are at the core of the AI-3/Epi/NE …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
… humans and other vertebrates. To delineate pathotypes, the genomes of twenty-eight <em>Aeromonas </em>isolates<em> </em>were screened by PCR to determine the presence of virulence factors including: aerolysin (<em>aerA</em>)<em>, </em>cytotoxic enterotoxin (<em>act</em>), hemolysin …
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Coordinate Regulation of Salmonella Virulence Gene Expression During Infection
… from mild gastroenteritis to life threatening systemic infections. An important step during a S. typhimurium infection is the invasion of non-phagocytic epithelial cells. Invasion is mediated by a Type Three Secretion System (TTSS), which is encoded on Salmonella Pathogenicity Island 1 (SPI1). …
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The WXXXE Effector Map Functions as a Potent and Specific Guanine Nucleotide Exchange Factor for CDC42
Many gram negative bacterial pathogens utilize a type three secretion system (TTSS) in order to infect eukaryotic cells. A TTSS looks and acts like a molecular syringe, allowing bacteria to inject effector proteins directly into the host cell cytoplasm. These effectors commandeer and manipulate …
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Elucidating three novel mechanisms of Pseudomonas syringae pathogenicity
… primarily because the plant innate immune system is capable of recognizing conserved microbial-associated molecular patterns (MAMPs). Adapted pathovars of P. syringae secrete effector proteins through a Type Three Secretion System (T3SS) to suppress the immune response elicited by their …
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Structural and Functional Studies of Sensor Kinase RetS from Pseudomonas aeruginosa and Peptidoglycan Hydrolase SleB from Bacillus anthracis
… Acute infections require a functional Type Three Secretion System (TTSS), which mediates the translocation of select cytotoxins into host cells. Chronic infections, the leading cause of death among cystic fibrosis patients, are characterized by drug-resistant biofilms formation. To …
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Dominant Suppression of Early Innate Immune Mechanisms by Yersinia pestis
… can interact with a myriad of different cell types, but the initial bacterial / host cell interactions during pulmonary infection are largely uncharacterized. I found that <italic>Y. pestis</italic> cannot be lavaged from the lung during the initial stages of infection but is freely lavageable …
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Regulation of virulence in Salmonella enterica
… genes involved in both invasion and the systemic stages of infection. Salmonella uses the Salmonella Pathogenicity Island I (SPI1) Type Three Secretion System (T3SS) to invade intestinal epithelia in the distal ileum of the host small intestine. HilA, a master regulator of the system, …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… diarrhea and epithelial invasion using a Type Three Secretion System (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The SPI1 T3SS directly injects several effector proteins into host cytosols, resulting in host actin rearrangement and engulfment of the bacteria. HilA activates …
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