Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 78 for “"synaptic vesicle"”.
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Exploring Synaptic Vesicle Exocytosis
… optical techniques to study determinants of presynaptic efficiency. Our methods are largely based on a chimera of a pH-sensitive variant of the green fluorescent protein and the vesicular glutamate transporter. This reporter is incorporated into synaptic vesicles and increases its fluorescence …
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Dissecting Synaptic Vesicle Endocytosis
<p>Synaptic vesicle endocytosis is critical for ensuring rapid local recycling of synaptic vesicles to enable ongoing neuronal activity. We utilized the sensitivity and robustness of the pHluorin assay of synaptic vesicle recycling to probe the control of endocytosis spatially and in response to …
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Calcium Triggered Synaptic Vesicle Exocytosis
… questions in neuroscience is how Ca 2+ promotes synaptic vesicles from rest to fusion leading to release of neurotransmitters. In this thesis, I first addressed if synaptogmin-1/SNARE binding is important for synaptic vesicle release. Using two knock-in mouse lines each with single amino-acid …
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Genetic Dissection of Synaptic Vesicle Endocytosis
Synaptic transmission is mediated by the quantal release of neurotransmitters through the fusion of discrete synaptic vesicles with the presynaptic membrane. To maintain reliable transmission, synaptic vesicles and proteins must be recycled after release of neurotransmitters. A key protein in this …
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Molecular Mechanisms Controlling Synaptic Vesicle Fusion
… fusion events occur immediately upon a vesicle interacting with its target membrane. But, in certain circumstances, such as in synaptic vesicles, spontaneous fusion is not desired, so a clamp protein is necessary to prevent this fusion until signaled to do otherwise. In synapses, this …
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Synaptic vesicle recycling <i>in Vivo</i>
Die Grundlagen der Reizweiterleitung an Synapsen sind prinzipiell gut verstanden: Wenn ein Aktionspotenzial die Synapse erreicht, induziert es einen Kalziumeinstrom, der wiederum zur Fusion synaptischer Vesikel mit der Plasmamembran führt, woraufhin die in den Vesikeln enthaltenen Neurotransmitter …
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Regulation of Synaptic Vesicle Trafficking at Central Synapses
… inter-neuronal communication in the brain. At presynaptic terminals, synaptic vesicles fuse with plasma membrane in response to electrical stimulation, followed by rapid retrieval to the terminal and re-organization for reuse. Thus, synaptic vesicle trafficking is of interest as to where …
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Membrane Lipids and Synaptic Vesicle Trafficking in the CNS
Most vesicles within a synapse are dormant. The rest participate in synaptic neurotransmission, with a portion of these preferentially fusing first. Moreover, all synapses experience spontaneous neurotransmitter release which may originate from the random exocytosis of vesicles prepared to fuse …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
Synaptic vesicle recycling is essential for maintaining normal synaptic function. The reuse of vesicles maintains the size of the presynaptic terminal and ensures the availability of synaptic vesicles for subsequent exocytosis. The coupling of exocytosis and endocytosis allows for continued rapid …
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Genetic analysis of synaptogyrin function in the synaptic vesicle cycle
… relies on the continual replenishment of synaptic vesicles that are primed for neurotransmitter release in response to action potentials. A vast array of proteins is required to mediate synaptic vesicle biogenesis, trafficking, docking, exocytosis, and endocytosis. Synaptogyrin and …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… brain, neurons communicate with each other by synaptic transmission. This process includes release of neurotransmitter from vesicles in the presynaptic neuron into the synaptic cleft, and sensing of these neurotransmitters by the postsynaptic neuron with specific receptors. Long-lasting changes …
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Synaptic vesicle protein 2A-dependent function and dysfunction at the presynapse
… for neuronal communication. At the presynapse, synaptic vesicles (SVs) undergo exocytosis to release neurotransmitter in response to incoming action potentials, and endocytosis to maintain the supply of SVs needed for further rounds of exocytosis. A key event during SV endocytosis is the …
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The Functional Organization of Synaptic Vesicle Pools In A Retinal Bipolar Neuron
… by ‘ribbon-like’ organelles that tether synaptic vesicles. The synaptic ribbons co-localize with sites of calcium entry and vesicle fusion, forming ribbon-style active zones. The ability of ribbon synapses to maintain rapid and sustained neurotransmission is critical for vision, hearing …
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Developmental trajectory of synaptic vesicle recycling in wild type and neurodevelopmental disorder mqodels
… This is made possible by exocytosis of synaptic vesicles (SV) that package the neurotransmitters, and these SVs are recycled via endocytosis. However, the sequence of events through which a presynapse acquires functionality is relatively poorly understood, which is surprising since …
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MUNC13 PROTEINS REGULATE STRAIN-DEPENDENT EFFECTS OF ETHANOL-MEDIATED INHIBITION OF SYNAPTIC VESICLE RECYCLING
Alcoholism is a chronically relapsing disorder characterized by an inability to remain abstinent from alcohol for prolonged periods of time. One of the major factors contributing to relapse is the development of profound anxiety during abstinence. Our lab has previously demonstrated that chronic …
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Mechanistic Insights into the Role of Munc13 in Synaptic Vesicle Docking, Priming, and Fusion
… communication that relies on the fusion of synaptic vesicles with the presynaptic membrane. These fusion events are tightly regulated by the influx of Ca2+, which is sensed by the complex protein machinery at the axon terminal. In order for these Ca2+-mediated fusion events to occur in the …
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The role of protein phosphorylation in the switching between modes of synaptic vesicle release
… stimulation and attention was focused on the presynaptic nerve terminal - by employing synaptosomes - for understanding the mechanisms occurring in synaptic vesicle exocytosis. Synaptic vesicles have rapid recycling capabilities and this in part may be due to a kiss-and-run mode of …
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Competition Between Synaptotagmin 1 and Complexin for SNARE Complex Binding, Controls Fast Synaptic Vesicle Exocytosis
… to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that were primed for release by SNARE complex assembly. Besides synaptotagmin 1, fast Ca2+- triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how …
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Molecular Anatomy of a Living Nerve Terminal: Actin and Synapsin in the Synaptic Vesicle Cycle
… is based on a series of events termed the synaptic vesicle cycle. Several molecular components contributing to the synaptic vesicle cycle are known, including the cytoskeletal protein actin and the synaptic vesicle-associated phosphoprotein synapsin. The studies presented in this thesis …
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Identification, Characterization, and Developmental Analyses of A Growth Cone Specific Epitope and a Synaptic Vesicle Protein
… a growth cone specific epitope (CD'A 1), a synaptic vesicle antigen (SVP38), and the developmental expression of each in various tissues. Although CD'A 1 and SVP38 do not have any direct relationship to each other, I will show that the expression of both changes over the same time period. …
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