Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"spliceosome"”.
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Structural studies of spliceosome assembly and catalysis
… by a dynamic macromolecular machine called the spliceosome. The spliceosome assembles anew on precursor mRNA (pre-mRNA) from its component small nuclear ribonucleoproteins (snRNPs) and protein factors. A series of conformational and compositional changes produces the catalytically active …
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Common genetic variation and spliceosome variants in rare developmental disorders
… probands with a pathogenic variant in the spliceosome. I found enrichment of differentially expressed genes for processes related to genes containing minor introns. Additionally, I found enrichment of genes involved in spliceosomal components among differentially spliced genes, suggesting a …
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Development of in vitro iCLIP techniques to study spliceosome remodelling by RNA helicases
… intronic sequence between two exons, the spliceosome is assembled stepwise on the pre-mRNA substrate. This ribonucleoprotein machine is extremely dynamic: both its activation and the progression through the catalytic stages require extensive compositional and structural remodelling. The …
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Formation of an Exon-Defined A Complex Spliceosome Intermediate Results in CD45 Exon Repression
… splicing. Pre-mRNA splicing is catalyzed by the spliceosome, a macromolecular complex that assembles de novo on a pre-mRNA template in a stepwise and highly dynamic process. The inherent dynamic nature of the spliceosome suggests the potential for regulation at numerous points along the assembly …
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The PHD finger protein 5 is a part of the spliceosome and acts as a DNA-binding protein
Vier Proteine: U2AF35, SRp40, mDomino und Ddx1 wurden durch Screening Yeast-two hybride-Bibliothek als Partner des PHF 5a-Protein gefunden. Proteininteraktionen wurden in-vitro und in-vivo bestätigt. Die für Bindungen verantwortlichen Domäne wurden als RS Domäne (reich an Arginin-Serin) …
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Conformation of the U12-U6atac snRNA Complex of the Minor Spliceosome and Binding by NTC-related Protein RBM22
… a mature mRNA. This process is catalyzed by the spliceosome, a multi-mega Dalton ribonucleoprotein complex assembled from five small nuclear ribonucleoproteins (snRNP) in the form of small nuclear (sn)RNA-protein complexes (U1, U2, U4, U5 and U6) and >100 proteins. snRNA components catalyze the …
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Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… human cells. Pre-mRNA splicing is catalyzed the spliceosome, which consists mainly of the U1, U2, U4, U5 and U6 snRNP, and about a hundred of non-snRNP proteins. Splicing regulators that bind to enhancer or silencer elements on the pre-mRNA can alter assembly of these spliceosome components. …
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Context-Dependent Function of the Splicing Factor hnRNP L
… RNA-based macromolecular enzyme known as the spliceosome. The spliceosome is made up of 5 small nuclear RNP (snRNP) complexes (U1, U2, U4/U6, and U5), all of which consist of a uridine-rich snRNA and multiple proteins. Importantly, the spliceosome is not a pre-formed enzyme but instead forms …
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The Role and Fate of Branch Site-U2 snRNA Interaction During Pre-mRNA Splicing
… remain unclear despite our deep knowledge of spliceosome composition. The complex assembly phase that precedes catalysis, and the involvement of several motifs in multiple interactions along the splicing pathway, exacerbate the difficulty of accessing splicing catalysis experimentally. snRNAs …
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Identification of Critical Residues in Spliceosomal Protein Dib1
… by a large macromolecular complex known as the spliceosome. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, and U6) and many associated proteins which help orient the pre-mRNA in such a way that catalyzes the splicing reactions. Of the associated proteins, Dib1 in S. cerevisiae is …
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Interaction of Spliceosomal U2 snRNP Protein p14 with Its Branch Site RNA Target
… catalyzed be a large macromolecular machine, the spliceosome, located in the nucleus of eukaryotic cells. The spliceosome is a protein-directed ribozyme composed of small nuclear RNAs (snRNA) and hundreds of proteins that assemble in a very dynamic process. One of these snRNAs, the U2 snRNA, is an …
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Étude d’un mécanisme d’induction de la sénescence par les défauts de la machinerie d’épissage
… Ce processus moléculaire est orchestré par le spliceosome, lui-même composé de 5 complexes ribonucléoprotéiques incluant plusieurs centaines de protéines. Chez les métazoaires, l’épissage est modulé par des protéines régulatrices de l’épissage qui contribuent à l’inhibition ou la facilitation …
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IDENTIFICATION OF SPLICING PATHWAY MUTATIONS VIA TARGETED SEQUENCING
… sites and catalytic removal of introns via the spliceosome is an essential and regulated component of eukaryotic gene expression. While ever-increasing numbers of human genetic diseases can be linked to defects in the splicing pathway, our molecular understanding of how these mutations disrupt …
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Characterization of splicing mechanisms by single-molecule fluorescence
… mechanistically group II introns are similar to spliceosome-catalyzed pre-mRNA splicing. Out of five snRNAs, only the highly conserved U2 and U6 snRNAs are required in both steps of RNA splicing. The U2-U6 snRNA complex forms the active site of the spliceosome and has been shown to undergo …
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Validating the WD Repeat Protein CDC40 as a Potential Therapeutic Target for Lung Cancer
… of CDC40 in lung cancer lines and found spliceosome components as the predominant binding partners for CDC40, further highlighting the major role of CDC40 in splicing. CDC40-CDC5 interaction, identified in the current thesis, was investigated using peptide displacement assay for drug …
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On co-transcriptional splicing and U6 snRNA biogenesis
… process of intron removal, is carried out in the spliceosome, a megacomplex comprehending up to 300 proteins. The core components of the spliceosome that directly interact with the pre-mRNA are the small nuclear ribonucleoprotein particles (snRNPs). They consist of one of the U-rich snRNAs U1, U2, …
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The splicing inhibitor Isoginkgetin leads to defects in multiple phases of the cell cycle
… activities related to inhibition of spliceosome assembly. The p53-tumour suppressor plays a prominent role in ell G1 and G2 cycle checkpoints and p53 is strongly activated in response to transcriptional stresses. Therefore, we determined the effect of p53 and Isoginkgetin treatment on …
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Co-transcriptional recruitment of the U1 snRNP
… establish an experimental system for monitoring spliceosome assembly in vivo, I have employed the chromatin immunoprecipitation (ChIP) assay to study co-transcriptional splicing events. Here, I use ChIP to examine key questions with respect to the recent proposal that RNA polymerase II (Pol II) …
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Single Molecule Studies Of Spliceosomal Snrnas U2-U6
<p>Spliceosomes catalyze the maturation of precursor mRNAs in organisms ranging</p> <p>from yeast to humans. Their catalytic core comprises three small nuclear RNAs (U2, U5</p> <p>and U6) involved in substrate positioning and catalysis. It has been postulated, but never</p> <p>shown experimentally, …
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ANALYSIS OF AXONAL TRANSPORT DEFECTS IN A ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY
… expressed protein that is involved in spliceosome assembly. A decrease in SMN protein levels leads to the dysregulation of splicing of downstream genes and thereby impairs cellular processes that most prominently affect MNs. Speculations as to the cause of this specific vulnerability of …
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