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Showing 1 to 20 of 79 for “"splenocytes"”.
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Immunotoxic and Oxidative Effects of Endosulfan and Permethrin on Murine SPlenocytes, in vitro
… of pesticides and their mixtures on murine splenocytes were evaluated. C57BL/6 male mouse splenocytes, in vitro, were exposed to permethrin and endosulfan, individually and in-combination (25-200 µM). The immunotoxic potential of these pesticides was monitored using a flow cytometric …
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ΑΝΟΣΟΑΠΟΚΡΙΣΕΙΣ ΦΥΣΙΟΛΟΓΙΚΩΝ ΚΑΙ ΑΡΘΡΙΤΙΚΩΝ ΑΡΟΥΡΑΙΩΝ ΣΕ ΔΙΑΦΟΡΑ ΑΝΤΙΓΟΝΑ ΚΑΙ ΜΙΤΟΓΟΝΑ
… THE MITOGENIC RESPONSES OF NORMAL OR AID RAT SPLENOCYTES TO LPS IS SIMILAR. IN CONTRAST THE MITOGENIC RESPONSE OF AID RAT SPLENOCYTES TO CON-A, 18-24 DAYS AFTER FREUD'S COMPLETE ADJUVANT (FCA) INJECTION (FOR AID INDUCTION) IS REDUCED COMPARED TO THE RESPONSE OF NORMAL RAT SPLENOCYTES. …
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Abnormal regulation of T lymphocytes from aged rats contributes to immunosenescence
… T lymphocytes, but unlike mouse lymphocytes, rat splenocytes and T lymphocytes proliferate in response to calcium ionophores in the absence of detectable IL 2 or IL 4. Proliferation in response to ionomycin and A23187 increases as early as 6 months of age and continues to rise until 24 months of …
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THE DEVELOPMENT OF SELECTIVE PHOTOSENSITIZERS TO TARGET PATHOGENIC T CELLS AND PREVENT GRAFT-VERSUS-HOST DISEASE
… depletion of alloimmune responses, donor C57BL/6 splenocytes were cocultured for 5 days with irradiated BALB/c splenocytes and then photodepleted (PD) with the best-in-class photosensitizer, 2-Se-Cl. PD-treated splenocytes were infused into lethally irradiated BALB/c (same-party) or C3H/HeJ …
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Reciprocal Relationships Between the Neuroendocrine System and Lymphoid Cells
… it is demonstrated that activation of porcine splenocytes with concanavalin A results in a two-fold increase in glucocorticoid and (beta)-adrenergic receptors with no change in the binding affinity of the receptor. Similarly, cortisol increases the number of (beta)-adrenoceptors on …
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Lymphocyte Activation, T-Cell Cytotoxicity and Their Relationship to Dietary Fat Enhanced Murine Mammary Tumorigenesis
… than mice fed 5% SBO. The response of isolated splenocytes to the T-cell mitogens, concanavalin A and phytohemagglutinin was 20%-25% lower in mice fed 20% SBO compared to mice fed 5% SBO after twenty weeks of feeding. There was no effect of SBO concentration on the splenocyte response to the …
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Estrogen Regulates Interferon-gamma (IFN-g) and IFN-g-Inducible iNOS Gene Expression: Implications to Immunity and Autoimmunity
… splenic lymphocytes. Impressively, we found that splenocytes from estrogen-treated mice when activated with Con-A also resulted in increased release of nitric oxide compared to placebo-treated mice. Furthermore, Con-A-activated splenocytes from estrogen-treated mice also had upregulated iNOS mRNA, …
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Generation of Baculovirus-Brucella Abortus Heat Shock Protein Recombinants; Mice Immune Responses Against the Recombinants, and B. Abortus Superoxide Dismutase and L7/L12 Recombinant Proteins
… blot), cell mediated (CMI, IFN-g- release by splenocytes, and CD3+CD4+, CD3+CD8+ T cell/ total splenocytes ratios) and protective immune responses of BALB/c mice (challenge with virulent <i>B. abortus</i> 2308) against these recombinants, against <i>B. abortus</i> superoxide dismutase (SOD) …
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Production of Monoclonal Antibodies for Toxoplasma gondii Lactate Dehydrogenase
… were immunized with synthetic peptides of TgLDH, splenocytes were harvested. To get the monoclonal antibodies (McAbs), splenocytes were fused with myeloma cells using PEG by a cell fusion technique. Hybridoma cells were selected by ELISA and subcloned through a limiting dilution method. McAbs were …
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Regulation of ERK1/2 and SAPK/JNK Phosphorylation by Histamine
… on ERK1/2 and SAPK/JNK phosphorylation in splenocytes.|C57BL/6 splenocytes were treated with different concentrations of histamine (10<sup>-4</sup> M to 10<sup>-11</sup> M). Histamine at higher concentration (10<sup>-4</sup> M) increased ERK2 phosphorylation. There was, however no …
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Structure, Function, and Diversity of Monoclonal Anti-Fluorescyl Antibodies
… (nine clones) were produced by fusion of BALB/c splenocytes and Sp 2/O-Ag 14 myeloma cells. Analyses of these clones were performed in order to compare structure/function relationships and to determine their prevalance in heterogeneous BALB/c immune sera.
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Studies on Lymphokine Activity in the Amphibian Rana Temporaria
… increasing tritiated thymidine uptake by splenocytes, Mitogenic factor or MF 6. Activity decreasing tritiated thymidine uptake by splenocytes, Inhibitor of DNA synthesis or IDS This is the first survey of lymphokine production by an ectothermic vertebrate, and shows that the properties, …
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CD200: A Novel Therapeutic Target for Chronic Lymphocytic Leukemia
… for its ability to support engraftment of CLL splenocytes in NOD.SCID mice. Infusion of sCD200hi CLL plasma, but not sCD200lo normal plasma, enhanced engraftment of CLL-splenocytes in vivo, an effect which was abrogated by depletion of sCD200 from CLL plasma. The prolonged engraftment of CLL …
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Immunotherapeutic alteration of tumor-induced suppression of interleukin 2 and 3 production by Propionibacterium acnes vaccination
… enhanced IL 2 production by N, N+V and T+V splenocytes, but failed to restore IL 2 production in TBH splenocyte cultures to normal levels. Thus, it appeared prostaglandins were not directly responsible for the majority of suppression seen in TBH. In the non-tumor-burdened host, prostaglandin …
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The effects of vitamin A deficiency on the immune system in a murine model of systemic lupus erythematosus
… receptor on the surface of the MRL/l mouse splenocytes, which resulted in an increased proliferation of normal T and B cells. To summarize, vitamin A deficiency decreases the overall progression of SLE in MRL/l mice. The mechanism includes an increased activity of suppressor T cells and an …
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ROLE OF THE MUCOSAE-ASSOCIATED EPITHELIAL CHEMOKINE (MEC/CCL28) IN THE MODULATION OF THE IMMUNE RESPONSE AGAINST VIRAL INFECTIONS
… surface receptors were evaluated on circulating splenocytes by flow cytometry. Th1- and Th2-type cytokine production was evaluated in the supernatants from cultivated splenocytes and mucosal T-cells after ex vivo re-stimulation with recombinant HIV-1IIIB gp120 or hemagglutinin (HA) from Influenza …
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Stimulation Through Tlr4 Increases Fviii Inhibitor Formation In A Mouse Model of Hemophilia A
… antigen presenting cells (APCs). Culture of splenocytes isolated from mice revealed that the combined stimulation of LPS and FVIII also synergistically increased early secretion of the inflammatory cytokines IL-6, TNF-α, and IL-10, which was not maintained throughout the course of the …
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The Isolation and Characterization of Growth Regulatory Factors Produced by a Herpes Simplex Virus Type 2 Transformed Mouse Tumor Cell Line, H238
… and a fourth containing inhibitory activity for splenocytes. The three peaks of mitogenic activity have been identified based on physiochemical characteristics: the first supported the anchorage-independent growth of EGF treated NRK-c-49 cells and resembles transforming growth factor-^ (TGF-/3); …
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Intracellular growth of Brucella abortus and B. melitensis in murine macrophage-like cell lines and partial characterization of a biologically active extract from B. abortus strain RB51
… a T helper type 1 (T<sub>H1</sub>) response, by splenocytes from mice vaccinated with strain RB51. An extract from strain RB51, designated S2, was found to stimulate the proliferation of splenic lymphocytes from strain RB51 vaccinated mice as well as the production of interleukin (IL)-2 and …
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Gene Repression and Cell Cycle Regulation by PU.1 in Acute Myeloid Leukemia
… NOD/SCID/γc-/- mice transplanted with Sfpi1BN/BN splenocytes become sick with disease resembling AML. Induction of PU.1 expression results in repression of the cell cycle regulator, E2F1, suggesting PU.1 represses E2F1 in order to enable cell cycle exit and differentiation. Understanding the …
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