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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"single chain variable fragment"”.
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Studies on voltage-gated sodium channel β3 subunit structure and cancer-related functions using single-chain variable fragment antibodies and bioinformatics
… tissues. This thesis describes the use of single-chain variable fragment (scFv) antibodies to study the binding site of the β3 subunit on the pain-sensing channel, Nav1.7, and investigate the role of the VGSC β3 subunit in cancer guided by bioinformatic analysis. *In silico* docking was …
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Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …
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Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we studied …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
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Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
… 항-DNA 항체 구조의 생화학적 특성 항-DNA 항체인 3D8 단일 가닥 가변부위 (single chain variable fragment: scFv)는 핵산결합능 및 가수분해활성이 있으며, 3D8 VL 단독부위만으로도 핵산 결합능 및 가수분해능을 발휘한다. 또한 3D8 scFv는 세포막 침투능이 있어서, 세포막을 투과한 후, 세포질에 위치하게 되고, 결국은 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 3D8 scFv의 핵산가수분해 활성은 그대로 유지하면서, 세포막 투과 후 핵으로 이동할 수 있는 3D8 scFv …
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Synergic Effect on Cell Death by Co-treatment with TRAIL and Human Anti-DR5 Antibody
… cells. In previous study we isolated a human single-chain variable fragment (scFv) antibody (HW1) that recognizes a different epitope on DR5 form that of TRAIL and induced autophagic cell death. when treated to various cancer cell lines. To study the interaction between TRAIL-induced apoptosis …
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Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies
… and aldolase (ALDO), recombinant trypanosome variable surface glycoprotein (VSG) and malignant catarrhal fever virus (MCFV) each chicken produced egg yolk antibodies (IgY) against four of the five antigens. Using phage display technology, two single-chain variable fragment (scFv) antibody …
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Investigating the Effects of Glycochenodeoxycholic Acid on Mutant Murine Norovirus P-Domain Structure and Antibody Interactions
… WU23. In the presence of GCDCA, we found variable dynamics across dimer pairs A/B and C/C. This result may support our hypothesis that WU23 is less sensitive to gastrointestinal cofactors such as bile salts. Additionally, we characterized the binding and neutralization capacity of three …
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Novel anitbody-based assays for disease and food contaminant detection
… Irish herds. A previously isolated Am1-specific single chain variable fragment (scFv) was converted to a chimeric Fab format by the addition of human constant regions, using PCR assembly methods. A mutant Fab fragment was also isolated and demonstrated improved assay performance when compared to …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it localizes in cytosol resulting in cell death by cellular rRNA degradation. These result mean that 3D8 scFv would also have …
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Development of monoclonal antibodies targeting T cell coinhibitory proteins TIM-3 and TIM-4
… been developed yet. In this study, monoclonal single chain variable fragment (scFv) antibodies against TIM-3-Fc and TIM-4 were identified using phage display biopanning which isolated antibodies from large immunoglobulin gene repertoires displayed on the surface of bacteriophages. Thirty …
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Plant-expressed diagnostic proteins and their use for the identification and differentiation of infected and vaccinated animals with foot-and-mouth disease virus
… reagent to be expressed: this was the CRAb-FM27 single chain variable fragment (scFv), which binds a 3B epitope on the 3ABC polyprotein and has previously shown to be effective as a competing antibody in a C-ELISA. The 3B antigen and the scFv were successfully expressed and purified from N. …
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Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing
… determining regions (CDRs; L3 from the light chain, H2 and H3 from the heavy chain), and were combinatorially cloned into a synthetic single chain variable fragment (scFv) framework for molecular display. Our novel CDR sequence design utilized a hidden Markov model (HMM) that was trained on …
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Monoclonal and phage-displayed antibodies as lipopolysaccharide serotyping reagents and their use in typing colony variants of Vibrio vulnificus
… by having DNA coding for its light and heavy chains cloned into a phage-display system. The published primers, designed to be used with the pComb3X display system, produced primer dimers and were unsuccessful in generating the single chain variable fragment (scFv) to be cloned into the phage …
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Development of a Dusky kob scFv gene phage display library for the discovery of antibodies to Brome mosaic virus - a proxy for a novel, emerging fish pathogen
… by a diverse repertoire of genes coding for the single chain variable fragment (scFv) of immunoglobulin M can be isolated and screened for affinity to a specific infectious agent or parasite. In this study, a phage display library displaying scFvs derived from combination pairings of Dusky kob …
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Development of nanobodies as tools to investigate G-quadruplex DNA secondary structures
… and much work has relied on G4 recognition by a single-chain variable fragment antibody, BG4. Other G4 probes have been reported but generally their usage has been limited to certain applications and in many cases the probes have limited selectivity for G4s. The aim of this thesis was to develop …
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Engineering scFv Antibodies for Point-of-Care Diagnostics
… measure serum CysC. By engineering CysC-specific single chain variable fragment (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used …
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Reversal of Neurodegeneration By Engineered Monocytes In Alzheimer’S Disease
… a scavenger receptor scaffold and an anti-o-Tau single-chain variable fragment. Our engineered CSR-monocytes not only mediate enhanced removal of extracellular o-Tau, but also uncouple phagocytosis from neurotoxic proinflammatory cytokine production. The majority of the monocyte-associated o-Tau …
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