Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 224 for “"shRNA"”.
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Design, Construction, and Screening of an shRNA Library Targeting Human Circular RNAs
… we developed a novel pooled library of ~ 15,000 shRNAs targeting ~ 5,000 circRNAs. We performed a loss-of-function screen with the circRNA shRNA library in a colorectal cancer cell line to systematically identify circRNAs that were required for cell proliferation and survival. During the …
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In vivo shRNA screen reveals PRMT5 as a key regulator in glioblastoma
… and demonstrate its feasibility for in vivo shRNA screening. We describe the results of parallel in vivo and in vitro shRNA screens to uncover epigenetic regulators with oncogenic functions and chemosensitizing potential in GBM. From these screens, the protein arginine methyltransferase PRMT5 …
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Model studies aimed toward constructing PI3K pathways in leukemia T-cells from shRNA knockdowns
… capture networks is unexplored. Motivated by a shRNA knockdown experiment on leukemia cell lines treated with a PI3K inhibitor, we sought to devise and test a method to reconstruct the relevant network, which could be used to gain a deeper understanding of the networks involved in PI3K signaling …
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In vivo pool-based shRNA screens to identify modulators of disease progression in hematopoietic malignancies
shRNA screens have been very effective in identifying novel cancer genes in mammalian cells, but they have primarily been limited to in vitro applications in tumor cell lines. Whereas in vivo retroviral mutagenesis screens typically identify gain-of-function alterations in positive selection …
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A Synthetic Lethal shRNA Screen and Genetic Proof of Concept Identifies RAC1 as a Novel Target to Disrupt Plexiform Neurofibroma Formation
… surgery or chemotherapies. I performed an shRNA library screen of patient-derived Schwann cell lines to identify novel therapeutic targets to disrupt PN formation and progression. An shRNA library screen of human kinases and Rho-GTPases was performed in NF1-/- and paired NF1 competent …
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Identification of mechanisms of gemcitabine resistance using a whole genome shRNA screen in a novel in vitro coculture model of pancreatic cancer.
… was used as the foundation of a genome-wide shRNA depletion screen to identify sensitizers of gemcitabine resistance in PDAC in vitro culture. The primary screen and subsequent validation screen identified 19 high confidence genes with potential translational value, of which knockdown …
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PROTECTION OF ISCHEMIA REPERFUSION INJURY IN MURINE LIVER BY GENE SILENCING OF COMPLEMENT 3
… 3 (C3) genes using a small hairpin RNA (shRNA) can potentially prevent liver IRI. Gene silencing of C3 in vivo was achieved via the administration of shRNA prior to IRI. Liver IRI was evaluated using histopathology, serum alanine aminotransferase (ALT), and aspartate aminotransferase …
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Growth Differentiation Factor 5 is a Paracrine Regulator of Sarcopenic Obesity
… with an empty vector control plasmid or a shRNA for Gdf5 knockdown. Western blot was used to measure Gdf5 protein expression. Myotube diameter were measured for each group. Markers for myogenic regulation (Cyclin D1, MyoD, MyoG), protein degradation (Atrogin, MuRF1) and inflammatory markers …
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IN VIVO AND IN VITRO EVALUATION OF THE COMBINATION OF RNA INTERFERING AND GENE THERAPY FOR TREATING MITOFUSIN2-RELATED DISEASES
… MFN2 mRNA are inhibited by a short hairpin RNA (shRNA), while the wild-type protein is restored by overexpressing a cDNA encoding functional MFN2 modified to be resistant to RNAi. We tested this strategy in vitro, in CMT2A patient-specific human induced pluripotent stem cell (iPSC)-differentiated …
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Optimization of molecular tools for high-throughput genetic screening
… tools for high-throughput screening, both for shRNA and sgRNA based reagents. Using large shRNA efficacy datasets, we first designed an algorithm predicting the potency of shRNAs based on sequence determinants. Combined with a novel shRNA backbone that further improves the processing of …
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Traitement contre le virus de l'anémie infectieuse du saumon en utilisant le système d'ARN interférant
… des traitements ciblant les virus. Une série de shRNA ciblant divers gènes de l'ISAV a été développée et le potentiel d'utiliser ces shRNA pour inhiber la réplication du virus de l'anémie infectieuse du saumon a été examiné. Les résultats ont démontrés qu'une méthode utilisant le …
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Desarrollo de sistema lentiviral para la codificación de un short hairpin RNA que permite el silenciamiento dual de las oncoproteínas E6 y E7 de HPV16 en células de pulmón humano
… de siRNA denominado short-hairpin RNA (shRNA), es un sistema eficiente de supresión de un transcrito blanco específico, que puede actuar durante varias generaciones celulares. Los vectores lentivirales son un sistema seguro y eficiente de empaquetamiento, capaces de infectar células …
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Investigation du rôle de pikfyve dans la progression tumorale des carcinomes rénaux
… à l'aide d'un système lentiviral inductible shRNA. Un test multiplex luminex a été réalisé afin d'analyser la concentration des facteurs de croissance, de cytokines et des métalloprotéinases matricielles (MMPs) présentent dans le milieu conditionné récolté des cellules exprimant le shRNA. De …
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Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
… vitro and in vivo utilizing a small hairpin RNA (shRNA) Tet-on system. Specifically, we have investigated the effects of clathrin-dependent endocytosis and caveolin-dependent endocytosis on the efficacy of PEI and PEI-derivative gene delivery in HeLa and MDA-MB-231 cell cultures and xenograft …
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Regulatory Balance Between the Peptide Trasporter, Pept1, and Amino Acid Transporter Gene Expression in the Enterocyte
… pursued. Potential cPepT1 overexpressing, cPepT1 shRNA or control shRNA expressing G0 chickens were generated by embryo injection of pseudolentiviral particles followed by ex ovo egg culture. Overall, 9 potential G0 cPepT1 overexpressing chickens, 15 potential G0 cPepT1 shRNA expressing chickens, …
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Engineering periodic short hairpin RNA delivery systems for enhanced therapeutic efficacy
… form of siRNA, or periodic short hairpin RNA (p-shRNA), as well as design a biodegradable polycationic carrier for efficient in vivo delivery of p-shRNA.
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A NOVEL METHOD TO INTERFERE WITH GENE EXPRESSION IN MICE
… vehicle bearing an U6-promoter dependent shRNA expression cassette. Lentiviruses are a sub-class of retroviruses that have the capability to infect non-dividing post-mitotic cells. Recently, in addition to their use to transform primary cells and established cell lines, lentiviruses have …
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A therapeutic approach to chronic myeloid leukaemia using short hairpin RNA molecules
… β3α2 and the β2α2 fusion points. These designed shRNA molecules were synthesized as <br/>oligonucleotides and were incorporated into a reverse PCR primer. Cassettes containing shRNA molecules and a respective promoter were produced by means of PCR and the products cloned into pB12mcs-‐eGFP …
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