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Showing 1 to 20 of 28 for “"pull-down assay"”.

  1. Probing cellular protein complexes using single-molecule pull-down

    Jain, AnkurUniversity of Illinois at Urbana-Champaign2014

    … interaction between biomolecules is the pull-down assay. We have extended the conventional pull-down assay to its ultimate limit: the analysis of single biomolecular complexes. We achieve this goal by performing the pull-downs directly on to the surface of microscope slides and …

    uiuc Repository record

  2. Screening auf Interaktionspartner für den hämatologischen Rezeptor PRV-1 : PRV-1 interagiert mit Decorin, C1qB und Defensin alpha 3

    Coch, Christoph

    … cDNA Bank durchgeführten Yeast Two Hybrid Assay hervorgegangene positive cDNA-Klone isoliert. Nach Sequenzierung wurden die Klone durch Abgleich mit der NCBI Datenbank identifiziert. Davon wurden acht cDNAs, die potentiell für eine in vivo Interaktion mit PRV-1 in Frage kommen, zur weiteren …

    freiburg-diss Repository record

  3. Identification of binding protein with kinesin18B

    김, 혜진2011

    … kinesin18B using a yeast two-hybrid system, GST pull-down assay, immunoprecipitation. This protein is Microtubule-associated protein RP/EB family member 1 containing a calponin homology(CH) domain at their N terminus and an EB1-like C-terminal motif at their C terminus : the functional importance …

    ajou Repository record

  4. DNA-Stabilität und Thioredoxin/Thioredoxin Reduktase im Zellkern

    Paunescu, KarinaUniversität Würzburg

    … Zudem wurde in einem Immunpräzipitationsansatz ("Pull-Down-Assay") nucläere Interaktionspartner des Enzyms charakterisiert. Diese Partner sollen nach Gelelektrophorese und MALDI-TOF-Analyse identifiziert werden. Zu den DNA-Prozessierungsenzyme zählt auch Topisomerase I. Durch Antikörpervermittelte …

    wurz-thes Repository record

  5. Characterisation of Androgen Receptor Variants in Breast Cancer and the Development of Thieno[2,3-b] Pyridines as Novel Anti-Cancer Compounds

    Alkheilewi, MohammadUniversity of Essex2019

    … of these compounds upon PCa. Using growth assays, flow-cytometry analysis, florescent imaging and cell tracking assays it was demonstrated that the compounds are potent inhibitors of PCa proliferation and motility. Importantly,the drugs were found to induce multi-nucleation, G2/M arrest and …

    essex Repository record

  6. Single-molecule profiling of tau aggregates in tauopathies: From test tube to patient

    Böken, DorotheaUniversity of Cambridge2025

    … MAPTau, a super-resolution Single-Molecule Pull-Down assay, and Single-Molecule Array (Simoa). Together, these tools enable the ultrasensitive detection and detailed characterisation of tau aggregates in brain and biofluid samples. Beyond quantification, they allow detailed profiling of …

    cambridge Repository record

  7. Biophysical characterization of KO513, a protein expressed in human invasive glioblastoma

    Nemukondeni, Ndivhuwo2020

    … of K0513. Using tryptophan fluorescence assay and limited proteolysis, nucleotides were found to have no significant effect on the tertiary structural conformation of K0513. Pull down assay shows promising interactors of K0513 from fibrosarcoma cell line; future studies will identify the …

    venda Repository record

  8. Targeting Traf6 For Cancer Therapeutical Development

    Morrow, John K2012

    … identified as hits were verified using a GST-pull down assay, comparing inhibition to a RANK decoy peptide. Since many drugs fail due to lack of efficacy and toxicity, predictive models for the evaluation of the LD50 and bioavailability of our TRAF6 hits, and these models can be used towards …

    uthsc Repository record

  9. GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae

    Sheen, Hyuk Ho2018

    … TBSV 5UTR as bait RNA and a streptotag-based RNA pull-down assay, GCD10 and GCD14 were identified as specific 5UTR-binding proteins. Subsequent electrophoretic mobility shift assay (EMSA) shows that GCD10 and GCD14 proteins bind directly to the 5 UTR and both subunits are required for the binding. …

    york Repository record

  10. Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators

    Wang, Stanley Y.University of Illinois at Urbana-Champaign2015

    … and rapid fluorescence anisotropy microplate assay (FAMA). The FAMA was applied to the binding of estrogen and progesterone receptors to their respective DNA response elements. The FAMA is also the first system suitable for screening large compound libraries to identify novel compounds that …

    uiuc Repository record

  11. New Instrumentation and Methods for Analyzing Biological Compounds for Drug Discovery and Discovering Novel Disease Markers

    Sandy, Kendall ElizabethThe University of Arizona.2018

    … the protein. These particles can be used in a pull-down assay for identification of ligand-receptor binding pairs. CHO-K1 cell membranes containing 5-HT1A receptors were tethered to sulfonate modified particles. NAN-190, a fluorescent 5-HT1A antagonist, binding to 5-HT1A receptors was monitored …

    arizona-thes Repository record

  12. Characterisation and Detection of P53 Aggregates

    Wu, YunzhaoUniversity of Cambridge2023

    … p53 aggregation employed microplate reader-based assays, which are limited in sensitivity and cannot provide morphological information about the aggregates. Hence, the biophysical properties of full-length p53 aggregates at physiological concentrations are not well characterised. Also, the …

    cambridge Repository record

  13. Postmeiotische Expression und funktionelle Charakterisierung von Lamin B3 in der Spermatogenese der Maus

    Schütz, WolfgangUniversität Würzburg

    … N-Terminus von Lamin B3 wurde über einen „Pull-Down-Assay“ nach möglichen Interaktionspartnern in Keimzellen gesucht. Mit MSY2, MSY2a und MSY4 wurden drei hoch interessante Kandidaten identifiziert. Sie gehören zu den Y-Box-Proteinen, DNA- und RNA-bindende Proteine, die bei der Speicherung …

    wurz-thes Repository record

  14. Identifizierung und Charakterisierung von Interaktionen der Nichtstrukturproteine NS1 und NS2 des Respiratorischen Synzytialvirus mit Proteinen der Wirtszelle

    Wolff, MichaelLudwig-Maximilians-Universität

    … p68 with both NS-Proteins was also shown in GST pull down assay in vitro. Engineered BRSV encoding a truncated p68 showed accelerated replication in MDBK and Vero cells, whereas growth of NS1/NS2 deletion mutants expressing the truncated p68 was unaffected. This indicates that the presence of …

    lmu-germany Repository record

  15. Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response

    김, 재진2016

    … 16 4. HR and NHEJ analysis 17 5. Neutral comet assay 18 6. Chromatin fractionation 18 7. pADPr polymer synthesis 19 8. pADPr and SUMO overlay assay 20 9. Dot blot 20 10. In vitro ubiquitination assay 20 11. In vivo ubiquitination assay 21 12. Western blot 22 13. Mass spectrometry analysis 22 14. …

    ajou Repository record

  16. Characterisation of LITAF, a protein associated with Charcot-Marie-Tooth disease type 1C

    Ho, Hon KwanUniversity of Cambridge

    … partners were screened using the traditional GST pull-down assay and the in-situ proximity labelling assay, BioID. A number of novel potential binding partners were identified in both assays. Among the list of potential binding partners, BAG3 was captured in both pull-down assays and was chosen …

    cambridge Repository record

  17. GENERATION OF FULL-LENGTH WILD-TYPE GATA4 PROTEIN AND CHARACTERIZATION OF ITS BINDING TO UNC-45 DOMAINS

    Anderson, Morgan2024

    … using protein-protein interaction techniques. Pull down assays suggested that GATA4 bound to the UCS domain of SM UNC-45, and possibly required flanking sequence in the central domain. Understanding this interaction can provide important mechanistic insight into how the SM UNC-45/GATA4 complex …

    sfasu Repository record

  18. CROSSTALK BETWEEN LYSOPHOSPATIDIC ACID (LPA) AND TRANSFORMING GROWTH FACTOR BETA (TGFβ) IN BREAST AND OVARIAN CANCER CELLS

    Wu, Jinhua2017

    … effect was most likely mediated through TGFβ downregulation of expression of LPA1, the major receptor subtype responsible for LPA-regulated cell migration. Knockdown of Smad3 or Smad4 with small hairpin RNA (shRNA) eliminated the inhibitory effects of TGFβ on the LPA1 expression and …

    vcu Repository record

  19. The regulation of the CAMP signalling pathway in the human pathogenic fungus, Paracoccidioides brasiliensis

    Janganan, T.K.Durham University2008

    … receptors (GPCR). Adenylyl cyclase acts downstream of these G proteins. Get subunits are required to regulate the activity of adenylyl cyclase (AC), which controls the level of cellular cAMP (lvey and Hoffman, 2005). Protein Kinase A (PKA), which is activated by cAMP, is required for …

    middlesex

  20. Part I: Steroid Receptor CO-Activator 3 (SRC-3) Expression in Lung Cancer and its Role in Regulating Cancer Cell Survival and Proliferation; Part II: Development of Phosphospecific Peptoid Ligand

    Cai, Di2010

    … phenotype of these cancers. siRNA-mediated down-regulation of SRC-3 in high-expressing tumor cells significantly inhibited tumor cell growth and induced apoptosis. The effect of SRC-3 down-regulation on cell phenotypes correlated with a cell line's endogenous expression level of the gene. …

    utswmed Repository record

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