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Showing 1 to 20 of 27 for “"provirus"”.
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Dna Iodination, Provirus Isolation, Excluded Volume and Electrostatic Effects on Dna Renaturation Rate
Made available in DSpace on 2014-12-13T20:09:44Z (GMT). No. of bitstreams: 1 7606897.pdf: 5366704 bytes, checksum: 6d42622fe957e10c86de00f621ad581c (MD5) Previous issue date: 1975
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ΜΗΧΑΝΙΣΜΟΙ ΟΓΚΟΓΕΝΕΣΗΣ ΔΙΑ ΤΟΥ ΛΕΥΧΑΙΜΟΓΟΝΟΥ ΙΟΥ ΤΟΥ MOLONEY (MOMULV)
… IN BOTH ITS EARLY AND LATE STAGES IS DUE TO PROVIRUS MEDIATES INSERTIONAL MUTAGENESIS. USING THE INTEGRATED PROVIRUS AS A MARKER WE HAVE IDENTIFIED AND CHARACTERIZED FIVE INDEPENDENT LOCI TOUR OF WHICH (MEVI-1,2,3,4) ARE INVOLVED IN TUMOR INDUCTION WHILE THE FIFTH ONE (TPL-1) IS INVOLVED IN …
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Regulation of Expression of a Retroviral Transforming Gene and its Cellular Homolog
… revertants (3 clones) have lost the complete FSV provirus. Type II revertant (1 clone) has a mutated FSV provirus which encodes a transformation defective protein of MW 130,000. This protein has no kinase activity in vitro, is underphosphorylated, and lacks phosphotyrosine in vivo. In …
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Cell intrinsic innate immunity protein RPRD2 restricts transcription from the HIV-1 LTR in human cell lines
… might also be involved in the restriction of HIV provirus transcription. Transient siRNA knockdown of RPRD2 increases transcription in 293T cells transfected with a GFP tagged HIV-1 LTR driven reported vector. Interestingly when RPRD2 is transiently knocked down in HeLa cells there is no effect on …
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Enrichment and Transcriptome Analysis of CD4+ T Cell Clones Harboring Intact HIV-1 Proviruses
… expanded cells harboring an intact HIV-1 provirus that does not require latency reversal is presented. The enrichment is based on CD45RA, a marker that distinguishes naïve from memory CD4+ T cells, as well as on the variable and constant domain of the T cell receptor (TCR) b chain, that …
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HETEROGENEITY OF LATENCY ESTABLISHMENT IN DIFFERENT HUMAN CD4+ T CELL SUBSETS STIMULATED WITH INTERLEUKIN-15
… ends in a transcriptionally silent integrated provirus. This phenotype was independent of differential expression of the positive transcription elongation factor b (P-TEFb) components Cyclin T1 and phospho/Cyclin-dependent kinase 9 (P/CDK9). When we analyzed the cellular distribution of the …
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Latency, Expression and Splicing During Infection With HIV
… virus (HIV-1). The mechanisms causing integrated provirus to become latent, the diversity of spliced viral transcripts and the cellular response to infection are not fully characterized and hinder the eradication of HIV-1. We applied high-throughput sequencing to investigate the effects of host …
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Strategies for Oncogenesis by Avian Leukosis Viruses
… derived from a single infected cell. Tumor proviruses were often defective and tumors often failed to express normal ALV mRNAs. Thus viral gene expression is not required for maintenance of neoplastic transformation. Most importantly, tumors from different birds had integration sites in …
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Single Cell Analysis of the HIV-1 Latent Reservoir
… a transcriptionally quiescent integrated HIV-1 provirus. As a result, interruption of suppressive therapy almost inevitably results in rapid viral rebound, which originates from these latently infected cells and prevents HIV-1 cure. It is thought that establishing the reservoir requires intact …
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Platinum and palladium complexes as human immunodeficiency virus latency reversal agents
The Persistence of latent HIV-1 provirus and reservoirs presents a formidable challenge in human immunodeficiency virus (HIV/AIDS), numerous efforts have been made to develop vaccines and chemotherapeutic agents. However, an effective vaccine remains elusive, and existing HIV/AIDS medications do …
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An Investigation of the SHIV reservoirs in the lymphoid organs of HIV-vaccinated rhesus monkeys after SHIV challenge
… loads indicating a larger amount of integrated provirus and potentially the presence of reservoirs within the macaques. While the low viral load values could mean there are few actively replicating viruses present in the PBMCs. The median of the averages for the RNA viral loads in the inguinal …
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Delineating Mechanisms of Signal-Induced RNA Polymerase II-Transcription
… transcription activation of the HIV-1 provirus. Decades of research has shown that HIV-1 transcription is primarily activated through pause release and transcription elongation by the HIV-1 transactivator Tat. Here, we use a novel Tat depletion approach to show that Tat function in …
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Enhancement of Hiv Persistence By Adipose Tissue
… (AT-SVF), activated memory CD4 T cells and provirus were readily detectable in all infected humans (n=5) and rhesus macaques (n=8) so far examined, and at frequencies comparable to other major viral tissue reservoirs. Thus, adipocytes have the capacity for promoting HIV replication, and …
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Mus dunni endogenous virus (MDEV)
… We provide evidence that the native MDEV provirus has only one repeat, and that these repeats are multiplied upon activation or passage of the virus through a process we call LTR expansion. This repeat number varies tremendously within an MDEV population, with viruses containing 3.15 to …
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Identification of differentially expressed genes in AHI-1-mediated leukemic transformation in cutaneous t-cell lymphoma
… oncogene Ahi-1 was recently identified through provirus insertional mutagenesis in murine leukemias and lymphomas. Its involvement in human leukemogenesis is demonstrated by gross perturbations in its expression in several leukemic cells lines, particularly in cutaneous T-cell lymphoma (CTCL) …
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Therapeutic Uses of Broadly Neutralizing Anti-HIV-1 Antibodies in Humanized Mice
… cells that harbor replication-competent provirus and are refractory to current therapies—termed the latent reservoir. New therapeutic approaches to clear latent HIV-1-infected cells are necessary to achieve cure. In the first part of this thesis, I show that HIV-1 mutates and diversifies …
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Investigation of the cellular tropism and in vivo distribution of HIV-1
… drug regime was halted. Characterisation of the provirus present in post mortem brain material could lead to a better understanding of this issue. The virus in the brain appears to mainly infect microglia and macrophages however it is not clear to what extent other brain cells might be infected. …
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Trans-Acting Factors Affecting Retroviral Recoding
… When cotransfected with Moloney murine leukemia provirus, the RPL4-mediated increase in readthrough reduces the amount of virus released. We also examined the effects of aminoglycoside drugs and the small molecule PTC124 on readthrough of the MoMLV gag-pol junction. We show that G418, paromomycin …
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Bovine leukemia virus infection in cattle: Viral distribution and immunogenetic associations with disease progression
… chain reaction (PCR) was employed to detect BLV provirus in each well. In five cows with and fourteen cows without persistent lymphocytosis (PL), BLV was found predominantly in CD5$\sp+$ and CD5$\sp-$ B-cells, with greater numbers of infected cells in the CD5$\sp+$ subset. Among non-PL cows the …
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Virus de la leucosis bovina (VLB) y evidencias de su potencial zoonótico
… estudios que reportan la detección del provirus en muestras de origen humano, y se propone como un potencial factor de riesgo para el desarrollo del cáncer de mama. Hasta el momento se desconoce cuál es la vía de transmisión del virus al humano, pero se plantea que podría tratarse de una …
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