Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 254 for “"proteomic analysis"”.
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Proteomic Analysis of Mammary Epithelial Cell Development
<p>In this set of studies, a proteomic approach was used to investigate the protein profile of the mammary epithelial cell (MEC) through different stages of mammary development. The HC11 cell line was used to investigate protein changes between undifferentiated and differentiated MEC, which …
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Comparative proteomic analysis of Australian epidemic Bordetella pertussis
… of the host’s immunisation background. Proteomic methods for secretome and surfaceome analysis was established. The B. pertussis secretome was characterised in Stainer-Scholte (SS) and Thalen-IJessel (THIJS) media. Ten virulence proteins were detected uniquely in THIJS while an …
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Proteomic analysis of genetically stratified grade-I meningioma
… stratified meningioma by characterising the proteomic landscape. Materials and methods: Frozen meningioma tissue samples were stratified according to mutational background: AKT1E17K/TRAF7, KLF4K409Q/TRAF7 and NF2-/-. For global proteomics, proteins were fractionated by SDS-PAGE followed by …
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Proteomic Analysis of Three Dimensional Organotypic Liver Models
… communication processes. Bottom-up shotgun proteomic analysis of the hepatic cells can lend insight into such processes. This technique employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) for relative quantification of protein abundances by measuring intensities of their …
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PROTEOMIC ANALYSIS OF TWO DIFFERENT STATES OF NAEGLERIA FOWLERI
… The purpose of this study is to create a proteomic signature map using two-dimensional gel electrophoresis (2-D gel) and recommend a subset of proteins that may be directly linked to the pathogenic state of N. fowleri. Replicates of 2-D Gels were created for both strains of N. fowleri and …
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Proteomic Analysis Delineates the Signaling Networks of Plasmodium falciparum
… approach for a system-wide quantitative analysis of protein expression and site-specific phosphorylation events of the Plasmodium asexual developmental cycle in the red blood cells. This study identified 2,767 proteins, 1,337 phosphoproteins, and 6,293 phosphorylation sites. Approximately …
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A Proteomic Analysis of Differentiation in the Mammary Epithelium
<p>While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is …
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
Pathogenic mycobacteria, like [Mycobacterium tuberculosis] M.tb and Mycobacterium avium (M.av), reside intracellularly in phagosomes where they are able to survive, because they block the process of phagosome maturation. ... An approach of characterizing M.av-containing phagosomes in terms of cell …
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Proteomic analysis of cellular models of neurodegeneration and mitochondrial dysfunction
… of a sub-cytotoxic concentration for further analysis. Using the previously optimised protocol for 2DE, mitochondrial preparations from differentiated N2a neuroblastoma cells treated with 1 mM MPTP for 24 hours were fractionated and compared to controls. Up to 32 proteins showed changes in …
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Proteomic analysis of diurnal variation in human skeletal muscle performance
… chain profile. Therefore, more sophisticated analysis is required. Proteomic techniques including 2-dimensional gel electrophoresis, high- performance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. …
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Proteomic Analysis of the Blood of <i>Alligator mississippiensis</i>
… alligator blood using mass spectrometry-based proteomics methods to understand their innate immune systems. The first goal was to sequence peptides and proteins from the blood serum and leukocytes using tandem mass spectrometry and <i>de novo</i> sequencing. The second goal was to determine the …
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Comparative Proteomic Analysis of Neem (Azadirachta indica) Seed and Leaves
… Family Meliaceae. This research focused on the Proteomic characterization of proteins from Neem leaves and Seeds and evaluated their anticancer potential in breast cancer cells. Despite consistent advancements in therapies, breast cancer remains a concerning health issue and a deadly disease …
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Transcriptomic and proteomic analysis of lycopene-overproducing Escherichia coli strains
… the small molecule lycopene. Global genomic and proteomic expression changes associated with increased lycopene production of mutant E. coli constructs were discovered using whole-genome DNA microarrays and a novel LC-MS technique, respectively. While most genes and proteins showed few expression …
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Proteomic Analysis of the Flavonoid Biosynthetic Machinery in Arabidopsis Thaliana
… spectrometry (MS) protocol developed for the analysis of complex peptide mixtures. An Agilent LC system coupled with an LTQ-MS instrument (Thermo Electron, San Jose, CA) was used for this purpose. Data analysis was performed with the Bioworks software package. Different optimization strategies …
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Proteomic analysis of biomarkers associated with immunotherapy in murine tumour models
Emergence of proteomics and high-throughput technologies has allowed the identification of protein expression patterns of disease that potentially hold clinical importance in predictive medicine. The analysis of complex data generated by these technologies incorporates the use of computer …
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Action potentials as indicators of metabolic perturbations for temporal proteomic analysis
… would enable us to perform non-invasive temporal proteomics. This model is capable of predicting and correlating the changes in cellular signaling, metabolic networks and action potential responses to metabolic perturbation. The neuronal-metabolic model was experimentally validated by performing …
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Proteomic Analysis of WNT Signaling and Metabolism of Fibrolamellar Hepatocellular Carcinoma
… may play a role based on the transcriptomic analysis, and synergistic mutagenesis screens. I will next utilize proteomics, imaging, and drug screening data which shows that Canonical Wnt signaling through β-catenin does not play a significant role in FLC. In the following chapter I will show …
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Structural and Dynamic Proximal Proteomic Analysis of TRPV2 Ion Channel Activation
Calcium signaling is a robust, tightly regulated pathway which mediates every vital cellular function through the propagation of localized calcium flux. Transient receptor potential vanilloid type 2 (TRPV2) ion channels are calcium-permeable, tetrameric ion channels with implications in several …
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Proteomic analysis of Pseudomonas putida F1 during growth on toluene-phenol mixtures
… mixtures on the growth of P. putida F1, a proteomics approach was utilized. An optimal proteome analysis procedure was developed and the proteomes of Pseudomonas putida F1 grown on either a single substrate (toluene or phenol) or a toluene-phenol mixture were analyzed by two-dimensional …
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