Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"polysomes"”.
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Hydrodynamics of Viruses and Polysomes
Made available in DSpace on 2014-12-09T17:35:53Z (GMT). No. of bitstreams: 1 6808070.pdf: 6678350 bytes, checksum: a1cf479c0ea385c1d117eee3fc6e6d76 (MD5) Previous issue date: 1967
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Host lncRNAs recognize invading bacteria and aid against infection by associating with polysomes
… been found to associate with ribosomes and polysomes; however, to date no research exists that addresses their role in translation regulation upon bacterial infection. AIM: The key question I wanted to answer during my PhD is whether host produced lncRNAs rewire the cell’s translation upon …
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Polysomes but not translation are required for estradiol-mediated stabilization of Xenopus laevis vitellogenin mRNA
… examining the distribution of the vitellogenin polysomes within total polysomes during estrogen induction, when the message is stabilized, and during withdrawal, when the message is destabilized. The vitellogenin mRNA was loaded with a full complement of ribosomes in both cases. This indicates …
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The Isolation of Friend Virus Protein by Zonal Centrifugation
… centrifugation was used to isolate Friend virus polysomes. The concentration of the polysomes and the removal of the sucrose was accomplished by ultrafiltration. Puromycin was used to release the nascent viral protein from the polysomes isolated, and the nascant protein was separated from the …
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L-dopa metabolism and the regulation of brain polysome aggregation
… mg/kg) caused a marked disaggregation of brain polysomes in immature rats {15-20 g body weight). Polysome disaggregation was maximal between 40 and 60 minutes after injection and not significant at 20 and 120 minutes. Older rats {50 and 100 g body weight) showed a similar response, but required …
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Investigation Of Posttranscriptional Regulation After Global Brain Ischemia And Reperfusion Injury
… neurons by pharmacological manipulation polysomes, 2. studied potential HuR posttranscriptional regulation mechanisms of facilitated nuclear export and polysome association, and 3. performed translation state analysis of reperfused neurons from hippocampal subregions CA1 and CA3. </p> …
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Ribonomic Control During Global Brain Ischemia And Reperfusion
… in CA1/CA3 in Hu protein immunoprecipitation and polysomes was HuB (Rel-N1). HuB is present in control CA3, 8 hr reperfused CA3, and 8 hr reperfused CA1, but absent from control CA1. AUF-1, hnRNP K, hnRNP M were also absent from control CA1 following Hu protein immunoprecipitation and Western …
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Surface Functionalizations towards Nucleic Acid Purification: a nanoscale study
… supramolecular complexes known as polyribosomes (polysomes).This project is aimed at developing and studying a miniaturized purification system able to isolate and extract polysome-associated mRNA, namely mRNA under active translation. The resulting microdevice will constitute a faster, simpler …
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Evidence for post-transcriptional regulation of induction of NADP- specific glutamate dehydrogenase by accumulation of its mRNA in uninduced synchronous Chlorella cells
… of a high yield of total undegraded cellular polysomes was developed. The crosslinking reagent, dimethyl suberimidate, was employed to prepare a stable NADP-GDH-crosslinked-Sepharose-4B antigen affinity column for the purification of rabbit anti-NADP-GDH IgG. Binding studies with ¹²⁵I-labelled …
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Regulating transcript fate through RNA elements in the 3' untranslated region
… ELAVL1 moves from chromatoid bodies to polysomes, facilitating translation. CPEB is a family of well studied proteins that takes advantage of the second type of mechanism of post-transcriptional regulation whereby it prevents the binding of translational machinery. It does this primarily …
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Mechanism of Translational Control by the Fragile X Mental Retardation Protein and Creation of the FMRP CTAG Mouse
… by acute biochemical removal of FMRP from polysomes, indicating reversibility of stalling. FMRP was also directly visualized on stalled polysomes by immunoelectron microscopy. Together, these results suggest a model in which FMRP actively regulates translation of target mRNAs by stalling …
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Functional Roles of Nova in RNA Metabolism
… structures, probably mRNPs, and with polysomes, suggesting a role for Nova in the regulation of cytoplasmic phenomena of RNA metabolism, such as mRNA localization and translation. These considerations prompted a gene expression screen aimed to identify differences in the pool of mRNAs …
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Studying sequence effects of mRNA 5' cap juxtapositions on translation initiation rate using randomization strategy of the extreme 5' end of mRNA
… density gradient to isolate mRNAs bound to polysomes. Based on the assumption that efficiently translated mRNAs are associated with polysomes, the effect of E5S on translation initiation was measured by comparing frequencies of nucleotides (and their combinations) at specific positions in …
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INVESTIGATING THE ROLE OF SPECIALIZED RIBOSOME DURING ERYTHROPOIESIS
… subsets of mRNAs. Through proteomics study of polysomes in differentiating erythroblasts, we show that the pool of ribosomes changes during erythropoiesis. Selective ribosome profiling show that certain subsets of mRNAs, including those important in erythropoiesis, are preferentially bound to …
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Commonalities between SMA and ALS: investigation of ribosome heterogeneity and translational defects
… in SMA. SMN co-sediments with ribosomes and polysomes and acts as a RAP, influencing the translation efficiency of specific mRNAs. Computational analyses identified specific features in the sequences of transcripts bound by SMN-primed ribosomes: translational enhancer sequences in the 5’ …
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ΦΩΣΦΟΡΥΛΙΩΣΗ ΠΡΩΤΕΙΝΩΝ ΣΕ ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙΠΟΝΤΙΚΟΥ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΜΙΑΣ ΝΕΑΣ ΚΙΝΑΣΗΣ ΠΡΩΤΕΙΝΩΝ ΠΟΥ ΡΥΘΜΙΖΕΤΑΙ ΑΠΟ ΚΥΚΛΙΚΑ ΝΟΥΚΛΕΟΤΙΔΙΑ
… FREE SYSTEM OF PROTEIN SYNTHESIS OF MOUSE LIVER (POLYSOMES,"PH5" FRACTION) AS WELL AS THE EFFECT OF VARIOUS AGENTS ON PROTEIN PHOSPHORYLATION IS REPORTED. THE ENZYME IS A SERINE- THREONINE KINASE WITH A MOLECULAR MASS OF 90 KDA. IT CONTAINS A 47 KDA AUTOPHOSPHORYLATABLE PEPTIDE AND CONSEQUENTLY IN …
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Translational defects in multiple tissues from the Smn2B/- mouse model of SMA.
… increase in SMN association with ribosomes/polysomes during postnatal development, indicating dynamic SMN function in protein translation during post-natal development. In SMA condition, where SMN protein levels drop, this binding reveals a tissue-specific decrease in the spinal cord and …
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ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΙΔΙΟΤΗΤΕΣ ΤΡΙΩΝ ΚΥΤΤΑΡΟΠΛΑΣΜΑΤΙΚΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ
… SALT, THEY ARE ATTACHED STRONGLY ON THE POLYSOMES.
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ISOLATION AND CHARACTERIZATION OF ARGINASE ISOZYMES FROM BOVINE LIVER AND BRAIN
… RNA preparation was also isolated from liver polysomes using immunological techniques.</p><p>Two liver and four brain arginases were purified, having similar native molecular weights, yet different subunit compositions, and displaying varying affinities for the Mn('2+) ion that is required for …
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Mechanisms of persistent translation arrest following global brain ischemia and reperfusion
… neither stress granules, processing bodies, or polysomes.</p> <p>5. mRNA granules were found to only colocalize with eIF4G, PABP and HuR, suggesting that mRNA granules may itself be related to or a subset of the ELAV/Hu granule.</p> <p>6. HuR co-localized to mRNA granules and translation of …
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