Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"phosphotransferase"”.
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Mutations of the glucose-pts in sublancin-resistant B. subtilis 168 ΔSPΒ
… the deletion of and mutations in the glucose phosphotransferase system confer resistance to sublancin. In this work we add to this knowledge by generating new sublancin-resistant mutants in B. subtilis 168 ΔSPβ. The mutations found support previous findings but also demonstrate the need to …
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IDENTIFICATION OF HCMV UL97 IN GBM CELL LINES AND A POSSIBLE ROLE FOR GANCICLOVIR
… to identify and examine the presence of HCMV phosphotransferase protein UL97. Reverse transcriptase polymerase chain reaction identified UL97 within two of the three cell lines, T98 and LN229. Western blotting confirmed that UL97 protein was being expressed and was present in both T98, and …
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A Study of the Effects of Polyethylene Glycol on Interactions Between Glycolytic Enzymes and F-Actin, and Among Glycolytic Enzymes
… (PGM, EC 5.4.2.1), ATP:3-phospho-D-glycerate 1-phosphotransferase (PGK, EC 2.7.2.3), 2-phospho-D-glycerate hydro-lyase (enolase, EC 4.2.1.11), D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate lyase (aldolase, 4.1.2.13), D-glyceraldehyde-3-phosphate:NAD$\sp{+}$ oxidoreductase (GAPDH, EC …
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The Role of Atypical PKC in the Plasticity of the Circadian Clock in the Rat Suprachiasmatic Nucleus
… C (aPKC) in GLU signaling. We found that the phosphotransferase activity of aPKC is increased significantly by GLU treatment to SCN brain slices at CT 14 in the early night. Disruption of aPKC phosphotransferase activity completely blocks GLU-induced phase delays in the early night, but not …
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The "atypical" protein kinase, SsoPK5, an archaeal member of the piD261/Bud32 subfamily
… period of time rendered SsoPK5 incapable of phosphotransferase activity. When stored at room temperature, SsoPK5 was capable of transferring the γ-phosphate from ATP to casein, reduced carboxyamidomethylated and maleylated (RCM) lysozyme,and p53. SsoPK5 phosphotransferase activity required a …
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Chemotactic Sensory Transduction in Bacillus Subtilis: A New Enzyme and a New Pathway
… substrates of the phosphoenolpyruvate-dependent-phosphotransferase system (PTS) in E. coli is accomplished using a chemoreceptor-independent signaling circuit in which phosphoryl flux through the PTS is monitored and used directly to modulate the activity of the chemotactic kinase. In contrast, …
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Elucidating Purinergic and Pro-inflammatory Signaling Mechanisms in Breast Cancer
… presented here shows that the expression and phosphotransferase activity of a pro-angiogenic nucleoside disphosphate kinase (NDPK) are markedly elevated in exosomes secreted by metastatic breast cancer cells. Moreover, these exosomes elicit pro-angiogenic and vascular remodeling events in …
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Regulation of HPr phosphorylation in Mycoplasma pneumoniae
… (HPrK/P) aus dem Phosphoenolpyruvat:Zucker-Phosphotransferase-System. In dieser Arbeit wurde das in vivo Aktivitätsprofil der HPrK/P unter verschiedenen Wachstumsbedingungen mit nativen Western-Blots untersucht. Die HPrK/P ist nur in Gegenwart von Glycerol als C-Quelle aktiv und …
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Enzyme Mediated Gentamicin Resistance Among Gram-Negative Clinical Isolates
… (13), and (c) a phosphorylating enzyme (phosphotransferase) (13).
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Biochemistry and regulation of CheA in Bacillus subtilis chemotaxis
… through a separate phosphoenolpyruvate-dependent phosphotransferase system (PTS), involved in sugar transport. In B. subtilis, we have determined that taxis toward PTS carbohydrates is mediated both by MCPs and the PTS. Finally, a restriction map of the distal end of the 26kb fla/che operon has …
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Microbial glucosinolate metabolism: Characterization of Enterococcus faecalis XL1 and functional analysis of a GH4 enzyme
… analyses identified multiple β-glucoside phosphotransferase system (PTS) transporters and glycoside hydrolases (GHs) responsive to sinigrin, with a GH4 enzyme Ef5510, showing the strongest induction. Functional studies revealed that Ef5510 is an NAD+/Mn2+-dependent hydrolase, and the …
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The dynamics of genetic networks
… the response of a cell to external stimuli. The phosphotransferase system of Escherichia coli regulates the metabolism of several sugars. We used a biochemical model of the system to investigate the range of possible metabolic responses it could generate.
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Identification and characterization of cell-adapted mutations in West Nile Virus and replicons
… (WNR) genomes were modified to contain neomycin phosphotransferase (antibiotic-resistance) gene and cells that persistently harbored this WNR were selected for using G418. There were many changes to the genomes harvested from WNR-bearing cell cultures compared to the parental genome, perhaps the …
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Die Rolle der Phosphatidylserin Decarboxylase für die mitochondriale Phospholipid-Biosynthese in Arabidopsis thaliana
… wird in Arabidopsis PE auch mittels Aminoalkohol-phosphotransferase synthetisiert. Der Verlust der gesamten PSD-Aktivität, wie es in der erzeugten psd Dreifachmutante der Fall ist, wirkt sich ausschließlich auf die Lipidzusammensetzung in der Mitochondrienmembran aus. Demzufolge wird …
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Untersuchungen zum Mechanismus endogener MHCKlasse- II-restringierter Antigenpräsentation
… Modellantigens Neomycin-Phosphotransferase (NeoR) und eines Modellantigen-spezifischen, MHC-Klasse-II-restringierten CD4+ T-Zell Klons wurde der endogene Präsentationsweg in nicht-professionellen und professionellen antigenpräsentierenden Zellen untersucht. Eine …
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Mutagenic Studies of RdoA, a Eukaryotic-Like SER/THR Protein Kinase in Salmonella Enterica Serovar Typhimurium
… to, choline kinase and aminoglycoside (3’) phosphotransferase IIIa (APH[3’]IIIa). These kinases all belong to a protein kinase superfamily and share highly conserved residues/motifs in their catalytic domain. In RdoA seven of these conserved amino acids were proposed to have functional roles …
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Analysis of a bacterial serine/threonine kinase
… to choline kinase and aminoglycoside 3’-phosphotransferase [APH(3’)]-IIIa which are both atypical kinases. RdoA and YihE have demonstrated the capacity for autophosphorylation in vitro and the ability to phosphorylate myelin basic protein, however, the native kinase target protein has not …
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Gentechnische Verfahren zur Erzeugung und Selektion von hochproduzierenden CHO-Zellen
… dass die Verwendung von mutierten Neomycin Phosphotransferase-Varianten als Selektionsmarker in CHO-DG44-Zellen für die Anreicherung von Hochproduzenten geeignet ist. Eine weitere Möglichkeit, die Expressionsrate eines stabil integrierten Produktgens zu erhöhen, ist der Einsatz von cis- und …
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Assembly and Display of Surface Proteins In Actinomyces Oris
… addition of glycopolymers, and I characterized a phosphotransferase enzyme with a novel role in protein glycosylation. These studies contribute to the understanding of the role of <em>A. oris</em> as a primary colonizer in the oral biofilm. Additionally, using <em>A. oris</em> as a model for …
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Structure and function of enzyme I of the PTS
The phosphoenolpyruvate: sugar phosphotransferase system (PTS) is responsible for the uptake and concomitant phosphorylation of many sugars in several species of bacteria. The first step of the PTS involves the phosphorylation of histidine containing phosphocarrier protein (HPr) by enzyme I (E.C. …
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