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Showing 1 to 20 of 56 for “"phosphite"”.
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Mechanistic Studies and Synthetic Applications of Phosphite Dehydrogenase
Cytochrome c oxidase, an enzyme that catalyzes the reduction of oxygen to water, has a binuclear center as well as a unique post-translational modification in the active site. Specifically, there is a crosslink between the nitrogen (Nepsilon2) of His240 and the carbon (Cepsilon 2) of Tyr244 …
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Compounds of Transition Metals With a Phosphite Ester
Made available in DSpace on 2014-12-05T19:37:33Z (GMT). No. of bitstreams: 1 6004009.pdf: 2920874 bytes, checksum: 33c294569d360fc18517cf0572aab9f1 (MD5) Previous issue date: 1960
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Kinetic Analysis and pH Dependence of the Phosphite Dehydrogenase Reaction
The pH-rate profiles for active site mutants Lys76Ala, Glu266G1n, and Arg237Lys are also bell-shaped and the higher pKa of each is 8.4, which is also consistent with that observed for the pH dependence of sulfite inhibition for the wild type enzyme. Interestingly, the acidic limb of the Glu266Gln …
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Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of Fr-900098
Another area in which biocatalysts are increasingly being used is in the production of small secondary metabolites, particularly antibiotics. Phosphonates are a small but growing class of compounds with many useful therapeutic properties. In particular, the phosphonates fosmidomycin and FR-900098 …
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Analyses of the in vitro effects of phosphite on Phytophthora cinnamomi isolates
… features, one of which is chemical control by phosphite. Despite its continuous use in South Africa for decades, there is yet to be a country-wide survey that assesses the current status of phosphite resistance in P. cinnamomi isolates. The European Union, which remains the greatest export …
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Understanding, Optimization, and Application of Phosphite Dehydrogenase: Advancing NAD(P)H Regeneration
NAD(P)H regeneration is an industrially important process that supports biocatalytic reactions that utilize these cofactors. PTDH shows promise for NAD(P)H regeneration and was therefore optimized for industrial application. The cofactor specificity of PTDH was relaxed by rational design using the …
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Mechanistic studies to determine the catalytic roles of active site residues in phosphite dehydrogenase
Phosphite dehydrogenase (PTDH) catalyzes the oxidation of phosphite to phosphate with the concurrent reduction of NAD+ to NADH. The mechanism of the reaction resembles a phosphoryl transfer reaction. A nucleophilic displacement reaction occurs on the phosphoryl group, with water or hydroxide …
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Genetic and Biochemical Characterization of a Phoa-Dependent Pathway for Phosphite Oxidation in Escherichia Coli
… biochemical characterization of a pathway for phosphite oxidation in Escherichia coli is described in this thesis work. Genetic comparison of different E. coli strains revealed that a phosphite oxidation pathway is dependent on the presence of bacterial alkaline phosphatase (BAP). Transposon …
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The effects of different concentrations of phosphite on ectomycorrhiza formation by Pisolithus tinctorius in Castanea dentata
Phosphites, salts of phosphorous acid, are used to combat the devastating root-rot diseases of American chestnut (Castanea dentata) and other woody plants caused by the Oomycete Phytophthora cinnamomi. However, phosphite treatments may negatively affect the plant’s ability to form ectomycorrhizas, …
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Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment
… diseases is the application of the chemical phosphite. Our understanding of the mechanism(s) underlying phosphite inhibition of Phytophthora diseases in plants is limited. Phosphite is known to have effects on both host plants and Phytophthora pathogens. In the present study, RNA-Seq was used …
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Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment
… diseases is the application of the chemical phosphite. Our understanding of the mechanism(s) underlying phosphite inhibition of Phytophthora diseases in plants is limited. Phosphite is known to have effects on both host plants and Phytophthora pathogens. In the present study, RNA-Seq was used …
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Sodium phosphate inhibition of the growth of selected foodborne spoilage yeasts
Sodium phosphite was evaluated for inhibition of growth of spoilage yeasts in laboratory media and in two commercial carbonated beverages. In addition, the effects of pH and atmosphere in combination with sodium phosphite were also examined in laboratory media. Inhibition studies in laboratory …
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Characterization of Reduced Phosphorus Metabolism in Alcaligenes Faecalis WM2072 and Xanthobacter Flavus WM2814
… these isolates that grew well using either hypophosphite or phosphite were identified by 16s rDNA analysis as a strain of Alcaligenes faecalis and a strain of Xanthobacter flavus. The genes required for oxidation of hypophosphite and phosphite by these organisms were identified using transposon …
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Characterization of fungicide resistance in grape powdery and downy mildew using field trials, bioassays, genomic, and transcriptomic approaches: quinoxyfen, phosphite, and mandipropamid
… grape pathogen, is commonly controlled by phosphite fungicides. A field trial and laboratory bioassays were conducted to determine whether P. viticola isolates from vineyards with suspected control failures showed reduced sensitivity against phosphite fungicides. Prophyt applied at 14-day …
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Biochemical and Genetic Characterization of Reduced Phosphorus Metabolism in Pseudomonas Stutzeri WM88
… Pseudomonas stutzeri WM88 is able to oxidize hypophosphite (P valence +1) and phosphite (P valence +3) for their use as the sole source of P. This process requires two operons encoded by htxABCDEFGHIJKLMN and ptxABCDE, which are separated by ca. 15 kbp. Sequence analysis of the htx operon suggests …
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SYNTHESIS, CHARACTERIZATION, AND THERMAL INVESTIGATION OF METAL PHOSPHITES AND POTENTIAL IMPLICATIONS FOR ASTROBIOLOGY
… early Earth. One possible explanation is that phosphite was a major source of reduced, reactive phosphorus on the early Earth and facilitated phosphorylation of biomolecules. Plausible sources of phosphite include meteoritic corrosion products and iron redox geochemistry in Archean oceans. The …
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Study of Copper Nanowires With Five-Twinned Structure Grown by Chemical Vapor Deposition and Their Applications
… is proposed to be the passivation by the phosphite, acting as a capping agent dissociated from the precursor, on {100} side planes of the CuNWs. CuNWs by CVD could also be selectively grown on a patterned Au/Si substrate. Single CuNW was selectively grown on the nanoscale-patterned …
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Rapid fabrication of polymers and composites via frontal polymerization
… We then discovered that the addition of alkyl phosphite inhibitors improved the pot life of the FROMP reaction by up to 140 fold. In addition to enabling an extended processing window for the liquid monomer solution, the alkyl phosphite inhibition facilitated FROMP of the gelled solution. Gels …
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Palladium catalysed oligomerization of 1-alkynes
… were replaced with bldentate amines or phosphite ligands. A one to one mixture of PPh and (CH ) P gave the highest yield of oligomers with 6-methylene-nona-4-yne as the major product. No aromatics were formed with any of the reaction systems studied. The general mechanism proposed, by …
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A Diverse Array of Synthetic Strategies for Phosphorus Group Transfer Chemistry: From Phosphinidenes to Phosphates
… mechanochemical approach to producing phosphite (HPO₃²⁻) via hydride-mediated reduction of condensed phosphates. Using potassium hydride, a range of inorganic phosphate sources—including pyrophosphate, triphosphate, trimetaphosphate, fluorophosphate, and polyphosphate—were converted to …
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