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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 323 for “"oligonucleotide"”.
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Non-Unique oligonucleotide probe selection heuristics
… consists of selecting both unique and nonunique oligonucleotide probes for oligonucleotide microarrays, which are widely used tools to identify viruses or bacteria in biological samples. The non-unique probes, designed to hybridize to at least one target, are used as alternatives when the design …
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Peptide conjugation to enhance oligonucleotide delivery
… altered to facilitate the delivery of antisense oligonucleotides. To accurately compare the existing CPPs, 64 CPP sequences were synthesized, conjugated to oligonucleotides, and assayed for delivery. These CPPs showed a range of effectiveness, with some CPPs hindering the delivery of …
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Oligonucleotide Design for Whole Genome Tiling Arrays
Oligonucleotides are short, single-stranded fragments of DNA or RNA, designed to readily bind with a unique part in the target sequence. They have many important applications including PCR (polymerase chain reaction) amplification, microarrays, or FISH (fluorescence in situ hybridization) probes. …
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Synthesis and applications of oligonucleotide-bound alginate hydrogels
… detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal and binding …
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Nucleoside and oligonucleotide approaches towards potential HIV chemotherapies
The use of oligonucleotides directed against the mRNA of HIV promises site-specific inhibition of viral replication. In this work, the effect of aralkyl substituents on oligonucleotide duplex stability was studied using model oligonucleotide sequences in an attempt to improve targeting of …
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Synthesis of oligonucleotide analogues for use in DNA nanostructures
… linear complement, using a third short oligonucleotide (ODN) as a helper for the<br/>circularisation of the second ODN. The copper(I)-catalysed cyclisation of<br/>oligonucleotides occurred by reaction between their terminal azide and their opposite<br/>terminal alkyne, to produce a …
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Metallkomplex-funktionalisierte Oligonucleotide und Peptidnucleinsäuren für die DNA-Sequenzerkennung
… die Synthese Metallkomplex-funktionalisierter Oligonucleotide und Peptidnucleinsäuren und deren Anwendung als Sonden für den sequenzspezifischen DNA-Nachweis. Die Ligation chemisch modifizierter Oligonucleotide (bzw. deren Analoga) am Nucleinsäure-Templat ist ein etabliertes Detektionsverfahren …
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Delivery and stability of antisense oligonucleotide conjugates in vitro
The efficacy of antisense oligonucleotide (ODN) therapy is dependent on four major parameters: delivery to cells, intracellular stability and localisation and efficient action at the target site.The aim of this project was to study the delivery of ODNs to macrophages and to assess the stability of …
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Algorithms for improving the design and production of oligonucleotide microarrays
… platforms exist, we focus on high-density oligonucleotide arrays, sometimes called DNA chips. One of the advantages of higher density arrays is that they allow the simultaneous measurement of the expression of several thousand genes at once, possibly covering all genes of a species in a …
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Next-Generation Oligonucleotide Probes For Transcriptome In Vivo Analysis (tiva)
… Analysis (TIVA), utilizes a light-activatable oligonucleotide probe to offer fine spatio-temporal control of mRNA capture. TIVA probes are highly modified RNA hairpins or loops incorporating a poly(U) “capture” sequence complementary to the poly(A) tail of mRNA, as well as a biotin moiety to …
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Oligonucleotide design and codon optimization for PCR-based gene synthesis
… reliable. DNA is synthesized from overlapping oligonucleotides by ligation or PCR; this thesis focuses on PCR-based methods. Many sets of oligonucleotides can be used to synthesize a given sequence; choosing the optimal set is a computational problem. A number of software tools for …
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Achieving Cell-Specific Delivery of Multiple Oligonucleotide Therapeutics with Aptamer Chimeras
… types of cancer cells, and delivers various oligonucleotide therapeutics. </p><p>The nucleolin aptamer targeted cancer cells by binding to membrane–associated nucleolin. Nucleolin, a conserved protein found in all eukaryotes, shuttles from the nucleus, through the cytoplasm to the cell …
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Synthesis of triplex-forming oligonucleotide conjugates of the anticancer drug temodal
… (Temodal) and mitozolomide to triplex-forming oligonucleotides (TFOs) is a potential way of targeting these alkylating agents to specific gene sequences to maximise site-selectivity. In this work, polypyrimidine TFO conjugates of both drugs were synthesised and targeted to duplex DNA in an …
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Oligonucleotide Binding Studies of Transfer Ribonucleic Acid Structure: Crystal and Solution
Made available in DSpace on 2014-12-14T04:27:42Z (GMT). No. of bitstreams: 1 7804053.pdf: 9334542 bytes, checksum: 97fb936a1b4500ec076253a65926f736 (MD5) Previous issue date: 1977
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Creating Scientific Software, with Application to Phylogenetics and Oligonucleotide Probe Design
The demands placed on scientific software are different from those placed on general purpose software, and as a result, creating software for science and for scientists requires a specialized approach. Much of software engineering practices have developed in situations in which a tool is desired to …
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Expanding the Scope of T3 DNA Ligase-Catalyzed Oligonucleotide Polymerizations (T3-LOOPER)
… incorporated into one polymer. Ligase-catalyzed oligonucleotide polymerization (LOOPER) is a method that synthesizes chemically modified nucleic acid libraries containing up to 16 unique chemical modifications. The method entails template directed ligations of chemically modified …
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Genetic Imbalances in Endometriosis Detected by Oligonucleotide-Array Based Comparative Genomic Hybridization
… tissue biopsies, and it was analyzed using oligonucleotide based array comparative genomic hybridization. The results suggest that an addition on chromosome 17p13.3 may play a role in the biological mechanisms involved in endometriosis as it was identified in 75% of the DNA samples obtained …
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