Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"mRNA transport"”.
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Mechanisms of dynein-based mRNA transport processes in human cells
… both space and time through the positioning of mRNA molecules in the cytoplasm. This process is commonly mediated by molecular motors, which transport mRNAs along the cytoskeleton to specific cellular regions. Despite the widespread occurrence of mRNA transport, the precise mechanisms by which …
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Identifizierung und partielle Charakterisierung eines neuen humanen RNA-Bindeproteins, RBP138, und seiner Isoformen
… ein neues RNA-Bindungsmotiv darstellt. Die mRNAs beider Proteine waren in sämtlichen untersuchten Geweben nur sehr niedrig exprimiert. Der Nachweis von endogenem RBP138 gelang in der Hepatomazellinie Huh7 nur nach vorausgegangener Immunpräzipitation. In Lokalisationsstudien wurde für beide …
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Funktionelle Analyse von Dendrin : zellbiologische Untersuchungen und Inaktivierung des Dendrin-Gens der Maus
… Amygdala, Striatum) exprimiert wird. Die mRNA, die für Dendrin kodiert, wird in die Dendriten der Neuronen transportiert. Wie bei den wenigen anderen bekannten dendritischen mRNAs nimmt man auch für Dendrin mRNA an, daß der mRNA-Transport in die Dendriten erfolgt, um auf spezifische …
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Single molecule pull down of double strand RNA binding proteins
… including RNA interference, anti-viral immunity, mRNA transport and alternative splicing. However, little is known about the molecular mechanisms underlying the interaction between dsRBPs and dsRNA. Here we examined four human dsRBPs, ADAD2, TRBP, Staufen 1 and ADAR1 `which have various numbers of …
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Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
… specifically with RNA in vitro, associates with mRNA in vivo, and directly interacts with the TAP mRNA export receptor. FRG1 also exists in a cytoplasmic pool that is dependent on an intact actin cytoskeleton for its localization and we demonstrate FRG1 itself is an actin binding protein. These …
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Modulation of Stress Granules formation: Role of mGlu5 receptor and FMRP and implications for pathophysiology of Fragile X Syndrome
… FMRP is an RNA-binding protein involved in mRNA transport and translation. Despite numerous studies, the available treatments are only symptomatic. There is no cure to replace FMRP expression, yet. FMRP can interact with RNA-binding proteins such as FXR1P, FXR2P, NUFIP and 82-FIP, and with …
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Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
… predominantly with components of the NPC, the mRNA transport and processing machinery, and the spindle pole body (SPB; the yeast microtubule organizing center). Structural and microscopic analyses show that the Mlp proteins may form the nuclear basket in yeast, as well as interconnect NPCs into …
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Elucidating the role of SRSF1, a prototypical splicing factor, in liver physiology
… multiple roles in RNA biology. This ranges from mRNA transport, translation regulation, non-sense mediated decay and even processing of miRNA. Therefore, it is not surprising that SRSF1 is vital for the survival of the cell. While deficiency in most tissues results in organ failure, the liver has …
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Die Isolierung und Charakterisierung der cDNA und des Gens Metr-1 der Maus, einem Vertreter der Bruno-like Genfamilie und Analysen zur Expression
… Regulation des Nervensystems, aber auch für den mRNA-Transport, die mRNA-Lokalisation und die mRNA-Translation sind sie von großer Bedeutung. Charakteristisch für die Struktur dieser Proteine sind drei RNA-Recognition-Motifs (RRM), die eine Kernstruktur einer funktionellen RNA-Bindungs-Domäne …
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Regulation of mGluR7 trafficking by SUMOylatio in neurons
… regulatory roles in synapse formation, axonal mRNA transport, channel activity, and receptorendocytosis. The metabotropic glutamate receptor type 7 (mGluR7), a presynaptic G protein-coupled receptor modulates excitatoryneurotransmission and synaptic plasticity by inhibiting neurotransmitter …
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Towards understanding the mechanisms behind mRNA localisation in growing axons
During embryonic nervous system assembly, mRNA localisation is precisely regulated in growing axons, affording subcellular autonomy by allowing controlled protein expression in space and time. Different sets of mRNAs exhibit different localisation patterns across the axon. Little is known, however, …
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Mechanism of mRNA localisation and posttranscriptional modification in Drosophila melanogaster embryonic neurons
… on post-transcriptional events. Two prevalent mRNA-based regulatory mechanisms in neurons are asymmetric mRNA localisation and the generation of different 3’UTR isoforms by alternative polyadenylation (APA). While experiments in mammalian systems indicate that subcellular mRNA localisation …
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A link between c. Elegans morphogenesis and mrna export
… nxf-1/TAP encodes a protein that is required for mRNA export from the nucleus to the cytoplasm. To better understand the role of NXF-1 in C. elegans embryonic morphogenesis, we analysed the expression of several apical junction markers in nxf-1(t2160) homozygous embryos and we showed misregulation …
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Study and engineering of nucleic acid-binding repeat proteins
… a PUF-based system for intracellular directional transport of mRNA was developed in mammalian cells. The biosynthetic device consists of a “motor”, which provides a directional movement, and the PUF protein that dimerizes with the “motor” in a ligand-dependent manner. The system allowed …