Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 24 for “"long terminal repeat"”.
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Regulation of the ETn/MusD family of active mouse long terminal repeat retrotransposons
Long terminal repeat (LTR) retrotransposons account for approximately 10% of mouse and 8% of human genomes and may play a role in modifying gene expression. Many species harbor retrotransposon families encompassing both autonomous and non-autonomous members. Specifically, the mouse Early Transposon …
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Studies on the Activity of the Long Terminal Repeat of Rous Sarcoma Virus in Animal Cells and in Yeast
… from the Rous sarcoma virus (RSV) Long terminal repeat (LTR) in untransformed rat 3Y1 fibroblasts is dependent on the presence of serum. Within an hour of addition of serum to a serum-deprived culture there is a 5 fold stimulation in the level of transcripts initiated at the LTR. …
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Directed evolution of an HIV-1 LTR specific recombinase for anti-retroviral therapy- a proof of concept study
… recognize and recombine a sequence from an HIV-1 Long Terminal Repeat (LTR), characterize the recombination proficiency of the evolved recombinase in mammalian cells and explore the potential of the recombinase for a novel antiretroviral strategy.
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Isolation and Characterization of a Full Length Retrotransposon: CR1
… in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent evolutionary time (St. John, 2004). A fully functional CR1 element itself is approximately 4.5kb long (Kajikawa, 1997), where almost all inserts are …
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Molekulare Charakterisierung des Retrotransposons Ylt1 der Hefe Yarrowia lipolytica
… besteht aus den flankierenden "Long Terminal Repeat" (LTR), zeta von jeweils 714 Nukleotiden und interner Region, eta von 8025 Nukleotiden. Deswegen baut die gesamte Sequenz von 9453 Nukleotiden. Der etwa Bereich enthält einen durchgängigen offenen Leserahm (ORF) (826 bis …
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Recombinant Expression, Purification, and Characterization of an HIV-1 Tat-Human Cyclin T1 Chimera
… trans-activation response (TAR) stem-loop of the long-terminal repeat (LTR) portion of nascent viral RNA. The production of viable transcripts is enhanced dramatically by the interaction of HIV-1 Tat with the host protein human Cyclin T1. Interaction with hCycT1 remodels Tat protein contributing a …
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Regulation of Pituitary Genes by the Transcription Factor, Pit-1, in the Domestic Turkey (A Turkey is NOT a Feathered Rat)
… under the control of the Avian Sarcoma Virus Long Terminal Repeat (ASVLTR) promoter. Cotransfection analyses in mouse L cells indicate that tPit-1* activates the tPrl, tGH, tPit-1 and rPrl promoters 4.6-, 3.8-, 1.7-, and 29.0-fold, respectively. Similar results were observed when …
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Non-LTR Retrotransposons in Mosquitoes: Diversity, Evolution, and Analysis of Potentially Active Elements
This research focuses on non-Long Terminal Repeat (non-LTR) retrotransposons in the African malaria mosquito, Anopheles gambiae and other mosquito species. An unprecedented diversity of non-LTRs was discovered by genome analysis of the An. gambiae genome assembly. One hundred and four families were …
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Retrotransposon mediated genomic fluidity in the human and chimpanzee lineages
LINE-1s (Long interspersed elements or L1s) and Alus are highly successful non-long terminal repeat retrotransposons with copy numbers of ~520,000 and >1 million within the human genome, respectively. They are associated with human genetic variation and genomic rearrangement. Although they are …
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The Role of Insulin-like Growth Factor-I and IGF-binding Proteins in Mammary Gland Development
… was targeted by the mouse mammary tumor virus - long terminal repeat (MMTV-LTR) to the mammary glands of two lines (15 and 29) of transgenic mice. Mammary synthesis of recombinant IGF-I increased the frequency of appearance of mammary alveolar buds (71% vs. 21%) in transgenic compared with …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… the control of the mouse mammary tumor virus-long terminal repeat (pMMTV-IGF-I), early simian virus (pSV40-IGF-I), and herpes simplex thymidine kinase (pTK-IGF-I) promoters. Stably transfected clones were generated by cotransfection of clonal MAC-T cells with the IGF-I expression vectors and a …
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Novel proapoptotic p63 isoforms are driven by an endogenous retrovirus in the male germ line of humans and great apes, likely increasing genome stability
… stimmt interessanterweise mit einem LTR (,long terminal repeat') des endogenen Retrovirus ERV9 überein. Die Insertion des ERV9-LTR vor den p63-Locus ist nur in Menschen und Menschenaffen (Hominidae) zu finden. Deshalb nehmen wir an, dass die retrovirale Integration sich sehr spät in der …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… sequence of a retrotransposon such as the long terminal repeat (LTR) sequence of Tst1. Parental monoploids, SH and various Solanaceae were evaluated by RD. Fluorescently-labeled retrotransposon-based primers were used in the ALFexpress automated fragment analyzer system. Eleven probes from …
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Characterization and Engineering of Transposons for Genome Editing
… to an attachment site. Next, we engineer a non-long terminal repeat (LTR) retrotransposon, R2 from the silkworm Bombyx mori (R2bm), as a site-specific DNA insertion tool in human cells. We show that R2bm can be used to perform DNA insertion into human 28S ribosomal DNA (rDNA) repeats and …
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Mosquito Transposable Elements and piwi Genes
… and their regulation. The first subject is on a Long Terminal Repeat (LTR) retrotransposon in the African malaria mosquito, Anopheles gambiae, namely Belly. The second subject focuses on the characterization of piwi genes in the dengue and yellow fever mosquito, Aedes aegypti. For the first …
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Viral and Host Factors Associated with HIV-1 Vertical Transmission and Pathogenesis
… I have molecularly characterized the HIV-1 long terminal repeat (LTR) from 6 mother-infant HIV-1 infected pairs, and shown that mutations generated during vertical transmission correlate with HIV-1 gene expression. Furthermore, I have also shown that there was a low degree of viral …
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Genetic analysis of reproductive and nut traits in almond [Prunus dulcis (Mill.) D.A. Webb]
… multi-allelic locus, S, which is about 70,000 bp long. A nearly complete sequence for the entire S locus sequence has been available only for the S₇ haplotype. In this research, next-generation sequencing technology was implemented to sequence the entire S locus simultaneously from 15 haplotypes. …
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The Influence of HIV-1 Subtype C LTR Genotype on Latency Potential
… regulatory elements of the HIV-1 promoter, the long terminal repeat (LTR), has been shown to influence latency establishment in in vitro models. We investigated the influence of inter-participant subtype C LTR genotypic variation on the establishment of latency in a dual reporter HIV-1 plasmid …
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Role of endogenous retrovirus in control of feline leukemia virus infection and implications for cross species transmission
… replication. Additionally, we documented enFeLV long terminal repeats (LTR) in domestic cats are negatively correlated to FeLV infection outcomes in vitro. Natural FeLV infections in both Florida panther and domestic cat tissues offered us the opportunity to examine end stage disease dynamics, …
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The contribution of repeat associated small RNAs to genetic variation, hybrid vigor and inbreeding depression in maize
… RNAs (siRNAs) that silence abundantly repeated sequences such as TEs. In contrast to miRNAs and as observed previously in both Arabidopsis and rice, hybridization passes on parental differences in siRNA populations, and for those 24-nt siRNAs that do differ between parents, there is a …
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