Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"inoculation method"”.
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Evaluation of an inoculation method and quantitative trait loci for fusarium head blight resistance in wheat
… in IL97-1828. In the second study, two different inoculation methods for the evaluation of FHB resistance were compared. In one method, the spray and bag method, a bag was placed over wheat heads for 24 h that had been inoculated using a conidia suspension. The second method, a grain spawn method, …
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Characterization, development of a field inoculation method, and fungicide sensitivity screening of the Pythium blight pathogen of snap bean (Phaseolus vulgaris L.)
New Jersey, Georgia, and the Eastern Shore of Virginia (ESV) are important snap bean (Phaseolus vulgaris L.) growing regions, but profitability is threatened by Pythium blight. Causal agents of Pythium blight on snap bean were identified using morphological characterization and sequence analysis of …
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A greenhouse screening method for resistance to gray leaf spot in maize
… mid-Atlantic states. A new greenhouse assay method with filter paper discs of C. zeaemaydis mycelia has been developed to evaluate corn germplasm for resistance to GLS. Mycelial inoculum obtained from cultures of mycelia in liquid malt media was pipetted at 100 ul samples onto each filter …
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Expression and inheritance of resistance to Fusarium solani in soybean
… of SDS on soybean, develop a greenhouse inoculation method for F. solani, then study the relationship between the greenhouse and field reactions, and study the inheritance of resistance to F. solani.
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Evaluations of soybean genotypes for drought tolerance and charcoal rot resistance
… of SoyNAM parents were evaluated by a cut-stem inoculation method in controlled conditions in a growth chamber. Soybean genotypes were significant (P < 0.001) for the percentage of dead plants. The parents were then tested with cutstem inoculation incorporated with and without a drought …
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An investigation of Fusarium verticillioides infection in maize using physiological and molecular approaches
… with F. verticillioides using a soak-seed inoculation method and grown for two weeks under controlled conditions. Analysis of the morphological characteristics showed that compared to the control (mock-infected) maize, infected maize seedlings displayed signs of stunting with leaves shorter …
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Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction
… The purpose of this research was to develop a method to assess infection of soybean roots by H. glycines with real-time quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a …
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The role of vector-derived microbiota and co-localization in the dissemination of vector transmitted pathogens
… fly vector. This study investigated the role of inoculation method and the vector, sand fly gut-derived microbiota in modulating the host immune response and facilitating parasite dissemination. Utilizing a novel skin prick model that closely mimics natural sand fly transmission, we explored the …
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Plant-produced, trans-encapsidated, replicative viral nanoparticles as a vaccine platform.
… these nanoparticle vaccines in planta via agroinoculation. To further explore the possibility of increasing the yield of plant-produced nanoparticle vaccines, an optimized spray-on inoculation method was developed using a low-toxicity surfactant to aid agro-inoculation. Unexpectedly, this also …
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Evaluation of Downy Mildew (Peronospora farinosa f.sp. chenopodii) Resistance among Quinoa Genotypes and Investigation of P. farinosa Growth using Scanning Electron Microscopy
… some of which exhibit mildew resistance. Methods for evaluating mildew severity are important for finding resistant genotypes that are useful in breeding programs. The main objectives of this study were to evaluate and investigate downy mildew resistance in quinoa through several different …
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Qualidade fisiológica de sementes de algodoeiro e comportamento in vitro de Sclerotinia sclerotiorum (Lib.) De Bary, submetido a diferentes temperaturas
… pathogens associated, 400 seeds were used, with inoculation method by direct contact of the seeds with mycelium and sclerotia. The sanitary quality of seeds was evaluated by the Blotter Test. The physiological quality was determined by the standard germination test and the evaluation of …
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Characterizing maize resistance to Clavibacter nebraskensis
… use of resistant hybrids is the primary control method for this disease. In this study, colonization and movement patterns of Cn during infection were examined using green fluorescent protein (GFP)-labeled bacterial strains. We successfully introduced a plasmid to Cn via electroporation, which …
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Tissue culture and transformation of introducing genes useful for pest management in rice
… to Liberty herbicide and Rhizoctonia solani. Methods were developed to screen transgenic plants for resistance to hygromycin B and Liberty herbicide using dip and cut in toxicant solutions. Five of 99 plants in the field test and 51 of 55 plants in greenhouse test were highly resistant to …
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Epidemiology of Ustilago bullata Berk. on Bromus tectorum L. and Implication for Biological Control
… cultivated fields as a function of seeding date, inoculation method, inoculum density, supplemental watering, and litter was also investigated. Teliospores germinated faster on potato dextrose agar than on water agar. Teliospores germinated slowly at temperatures far from the optimum of 15 and 20 …
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Investigations Into the Genetic Basis of Resistance to Brown Stem Rot in Soybean
… it has been successfully deployed as a method of control. Understanding the genetics of resistance to BSR and identifying new sources of resistance are key components of successful BSR control strategies. Objectives of these studies were to: screen 85 accessions from the germplasm …
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Effects of co-inoculation of Delftia acidovorans RAY209 on the growth of Bradyrhizobium diazoefficiens USDA110 inoculated Glycine max
… legume-rhizobia interaction, the co-inoculation of soybean with Bradyrhizobium and other plant PGPR has gain increasing popularity attributed to the observed synergistic effects that result in improved plant growth promotion and stress tolerance. The objective of the current …
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Evaluation of southern African maize germplasm for phytoalexin accumulation following inoculation by Fusarium verticillioides
… maize lines in response to F. verticillioides inoculation. Maize lines were inoculated with F. verticillioides using a seed soak inoculation method and grown in vitro for up to two weeks. The harvested tissue was analysed for fungal growth using quantitative PCR, putative phytoalexin …
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QTL mapping of Phytophthora root rot resistance and aphid resistance in soybean
… 7, in 2009 and 2010 using a modified rice grain inoculation method. The heritability of the PRR resistance ranged from 83% to 94%. A major locus contributing 50% to 76% of the phenotypic variation was detected within a 3 cM interval in the Rps1 region. Based on the specific responses to the …
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Dissection of black rot resistance towards marker-assisted breeding in grapevine Pedigree reconstruction and phenotyping optimization coupled with high-density linkage mapping revealed a major QTL associated with bunch resistance.
… exploited for breeding. Concurrently, a new inoculation method based on spore production from fresh infected leaves was developed. Ex vivo (detached leaves) inoculation did not reveal significant differences among the assessed genotypes and highlight-ed the tendency of spores to accumulate …