Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"human fibroblasts"”.
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DIRECT REPROGRAMMING OF HUMAN FIBROBLASTS INTO CARDIOMYOCYTES
Direct reprogramming of human fibroblasts into induced cardiomyocytes (iCMs) offers a potential therapeutic approach for cardiovascular disease yet hampered by low efficiency of human iCM generation. Here, we established an optimal culture condition by defining a chemically defined media (CDM). In …
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Evasion of interferon-gamma responses by Toxoplasma gondii in murine and human fibroblasts
… the IRGs to inhibit PV accumulation. However, humans lack interferon-inducible IRGs, and ROP5 and ROP18 do not affect Toxoplasma growth inhibition in human cells, suggesting these factors specifically evolved to battle the IRG system. Both ROP5 and the IRGs exhibit diversifying selection, and …
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Characterizing the Role of CDK2AP1 in Primary Human Fibroblasts and Human Embryonic Stem Cells
… the role of CDK2AP1 in somatic cells (primary human dermal fibroblasts (HDFs)) and human embryonic stem cells (hESCs) and specifically assess its impact on proliferation, self-renewal and differentiation. In the first part of this study, using a short-hairpin RNA (shRNA) approach, we …
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The Effects of Cell Culture Oxygen Levels on the Replicative Senescence Processes of Primary Human Fibroblasts
Serial passaging of primary human fibroblasts leads to the formation of non-dividing senescent cells by a process termed replicative senescence. This tissue culture-based methodology is currently used as a model system to determine the underlying mechanisms of in vivo cellular aging and tumor …
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Direct generation of region-specific induced neural Precursors from adult human fibroblasts using non-viral and viral methods
We previously demonstrated that adult human dermal fibroblasts (aHDFs) can be directly converted to neural precursor cells, and subsequently mature neurons and glia, by the introduction of the defined neural transcriptional factors SOX2 and PAX6. However, the understanding of the underlying gene …
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Exploring the global gene expression programs and regulation in the response of quiescent human fibroblasts to distinct proliferative stimuli
Serum treatment of quiescent human dermal fibroblasts induces proliferation coupled with a complex physiological response that is indicative of their normal role in wound-healing. However, it is not known to what extent such complex transcriptional events are specific to a given cell type and …
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The protective effects of the antioxidant combination of ferulic acid with vitamins C and E against UV-induced photodamage in human skin cells
… reduce the effect of UVA-induced photodamage in human epidermal (HaCaTs) and dermal skin cells (primary human fibroblasts (HFbs) and murine 3T3 cells). Pretreatment with the FA and vitamins C and E (vEC) combination solution reduced UVAinduced ROS in human fibroblasts while pre-treatment with vEC …
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TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
… that autophagosome formation is impaired in human fibroblasts from patients affected of mucolipidosis IV (MLIV; a severe lysosomal storage disorder caused by mutations in TRPML1). By using specific compound inhibitors during starvation, we found that TRPML1-mediated induction of autophagosome …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… by transfection with plasmids containing the human preproinsulin gene (hppI-1) and the GLUT2 gene for the glucose transporter isoform 2. Insulin secretion was responsive to changes in the glucose concentration up to about 50μM. Further studies to up-rate this glucose sensitivity into the mM …
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Differences in the regulation of Thrombospondin-1 expression between epithelial cells and fibroblasts
… proteins. It has been demonstrated that in human epithelial cells repression of the angiogenesis inhibitor Thrombospondin-1 (Tsp-1) requires stimulation of Myc in combination with hyper-physiologic levels of oncogenic Ras. This work demonstrates that in human mammary epithelial cells, …
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Purification and Characterization of Simian Virus 40 Large T Antigen
… was purified from a line of SV40 transformed human fibroblasts (SV80 cells) and its biochemical activities studied.
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PLASMA MEMBRANE ASSOCIATED GLYCOHYDROLASES: KINETICS PARAMETER CHARACTERIZATION AND BIOLOGICAL FUNCTION
… hydrolases and sphingolipid synthases (1, 2). In human fibroblasts it has been observed a crosstalk between the expression and the activity of membrane associated glycohydrolases (3), furthermore, qualitative and quantitative changes in glycosphingolipids, together with changes in the expression …
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Oncolytic adenovirus vectors for nitroreductase suicide gene therapy of prostate cancer
… showed some lytic activity to non-transformed human fibroblasts. Our collaborators at the Institut Català d'Oncologia designed a panel of oncolytic adenovirus vectors with the E1A CR2 24 deletion and the E1A promoter replaced by an insulated E2F-1 promoter. The latest two in this series of …
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Small Molecules Modulate Chromatin Accessibility to Promote NEUROG2-Mediated Fibroblast-to-Neuron Reprogramming
… the transdifferentiation of lineage-restricted fibroblasts into functional neurons; however, a mechanistic model of the immediate-early events that catalyze this conversion has not been well defined. We utilized a high-efficiency reprogramming system of NEUROG2, forskolin (F), and dorsomorphin …
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MOLECULAR BIOLOGY OF HEPATITIS B VIRUS DNA-CELL INTERACTION
… as a DNA-binding site. Cloned E2BP expressed in human fibroblasts by transient transfection displayed similar EII binding and activating characteristics as native E2BP in hepatocytes. This is the first report of the interaction of a hnRNP-binding protein with a viral enhancer element. The concept …
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Gene expression-based screening of inhibitors of signal transduction
… signature for PDGF activation of ERK pathway in human fibroblasts and used this signature to screen a chemical compound library for an inhibitor of PDGFR/ERK pathway. As a result of the screen, I identified aurintricarboxylic acid, ATA, as a new inhibitor of PDGF receptor. The success of this …
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Effects of oncogenic Ras and p38 mitogen-activated protein kinase on the adhesion of normal human cells
… mutations in RAS oncogenes commonly arise in human cancers. However, in experimental settings, oncogenic RAS has most often been studied at supraphysiological levels of expression. Importantly, work by others showed that the response of murine cells to expression of oncogenic ras from the …
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Citrulline metabolism in cultured fibroblasts : citrullinemia analysis and nitric oxide production
… the presence of the nitric oxide pathway in fibroblasts was investigated. Inducible nitric oxide synthase activity was assayed by measuring the production of ¹⁴C-citrulline from ¹⁴C-arginine after cytokine stimulation. By using the citrullinemic cell line (ASS deficient) any citrulline that …
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Integration and function of new neurons generated from fibroblasts and adult neural stem cells in the pathological brain
… in the sense that they were generated from fibroblasts. Papers One and Two focused on the new neurons generated from neural stem cells in the SVZ and SGZ after pathological insults. We investigated how the newly generated neurons migrate and integrate in the brain. These two phenomena, …
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