Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"glutaminase"”.
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Functional analyses of ζ-crystallin binding to the 3'-UTR of glutaminase (GA) mRNA
… is associated with increased expression of renal glutaminase (GA) that results from selective stabilization of the GA mRNA. LLC-PK1- FBPase+ cells, a pH-responsive porcine kidney cell line, express four distinct GA mRNAs, that are ~2.5, 3.5, 4.5, and 5.0 kb in length. RNase H mapping indicated …
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Characterization of the instability elements present within the 3' UTR of the glutaminase mRNA in WKPT kidney cells
During metabolic acidosis, the level of glutaminase (GA) is increased 8-fold within the renal proximal convoluted tubule. This adaptation contributes to the ability of the kidney to generate NH4+ and HCO3- ions from glutamine to facilitate the excretion of acids and partially restore acid-base …
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Deamidation of soy protein by protein-glutaminase: Process evaluation and effect of deamidation on protein functional properties and flavor-protein interactions
… of soy protein isolate (SPI) by protein-glutaminase (PG) was successfully carried out using response surface methodology (RSM) to obtain a deamidated SPI with high degree of deamidation (DD) and an acceptably low degree of hydrolysis (DH). The deamidated SPI had enhanced solubility in …
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EXPLORING THE ALLOSTERIC INHIBITION MECHANISM OF HUMAN KIDNEY TYPE GLUTAMINASE TO ARREST CANCER CELL PROLIFERATION AND INVESTIGATING HAKAI MEDIATED UBIQUITINATION TOWARDS CONTROLLING CANCER CELL MIGRATION
The development and progression of cancer can be associated with certain hallmarks. Two among them are the altered cell metabolism (Warburg effect), and cancer cell invasion/progression mediated by post-translational modulation of cell signaling. In this thesis we have attempted to explore these …
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Targeting Metabolic Adaptations to Anti-Angiogenic Therapy In Ovarian Cancer
… on glutamine metabolism, <em>via </em>the enzyme glutaminase (GLS), is a known mechanism of adaptive resistance.</p> <p><strong>Purpose: </strong>To assess the efficacy of a glutaminase inhibitor as a means of exploiting the metabolic vulnerability of glutamine dependence that develops as a result …
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Myc influences glutamine metabolism to induce autophagy in tumorigenesis
… the dependency of Myc-induced autophagy on glutaminase and major regulators of autophagy, such as TOR, Atg1, Atg5 and ammonia, a by-product of glutamine catabolism, by dissecting these mechanisms both in normal epithelial clones and hyperproliferating RasV12 -expressing cells. Our results …
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Studies Of The Molecular Mechanisms Underlying Cancer Stem Cells And The Transformed Metabolic Phenotype
… protein/crosslinking enzyme tissue transglutaminase (tTG), two proteins suspected to have key roles in tumor initiation and the development of the malignant state, in glioma stem cells (GSCs). Additionally, I examined the contributions of two isoforms of glutaminase (GLS), given the …
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Enzymatic Characterization Of The Ammonia Tunnel In Helicobacter Pylori Asp-Trnaasn/glu-Trnagln Amidotransferase
… the three activities of <i>H. pylori</i> AdT (glutaminase, kinase and transamidase) and used these reactions as probes to examine the inter-domain communication and ammonia delivery mechanisms between this enzyme's two isolated active sites. We adapted and optimized an assay to kinetically …
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Glutamine Antagonism and Its Utlity [sic] as a Therapeutic Modality in Cancer
… them to the effects of CB-839, a specific glutaminase inhibitor. DON is one of the oldest and well-known glutamine antagonists and can effectively limit tumor growth in a preclinical setting. Unfortunately, DON was removed from early phase clinical trials due to unacceptable toxicity in the …
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STRUCTURE-BASED COMPUTATIONAL MODELING OF PROTEIN-LIGAND INTERACTIONS - APPLIED TO PROTEINS INVOLVED IN HUMAN DISEASES
… an important cancer target, human kidney-type glutaminase, which identified novel binders that manifest inhibition potential.
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis: Iron-Sulfur Biochemistry and Enzymology
… assays: glutamine PRPP amidotransferase, glutaminase and PRPP hydrolase; these results demonstrated that the cluster does not have a redox function during catalysis of amide transfer by the enzyme.
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Targeting Mitochondrial Proline Dehydrogenase (PRODH) with a Suicide Inhibitor as a Novel Anticancer Strategy
… of 51 human breast cancer cell lines, PRODH and glutaminase (GLS1) expression levels were found to be inversely correlated (1) implicating two independent and alternative mitochondrial pathways supplying anaplerotic glutamate for cancer cell energy production and macromolecular synthesis. …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
… transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design and synthesis of amino acid analogs, based off of an L-aspartate or L-proline scaffold, to be evaluated as inhibitors of the amino acid transporters ASCT (subtypes 1 & 2) and the …
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SYNTHESIS OF N<sub>B</sub>-ARYL-ASPARTAMIDES, N<sub>a</sub>-ARYLAMIDE-ASPARTATES, AND HYDROXY-L-PROLINE DERIVATIVES AS INHIBITORS OF AMINO ACID TRANSPORT FOR EVALUATING THE GLUTAMINE / GLUTAMATE CYCLE
… transporters and amino acid converting enzymes, glutaminase and glutamine synthetase. This work details the design and synthesis of amino acid analogs, based off of an L-aspartate or L-proline scaffold, to be evaluated as inhibitors of the amino acid transporters ASCT (subtypes 1 & 2) and the …
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Channeling in purine biosynthesis : efforts to detect interactions between PurF and PurD and characterization of the FGAR-AT complex
… enzymes (ATs), channeling of NH3 between glutaminase and AT active sites has been implicated in the formylglycinamide ribonucleotide amidotransferase (FGAR-AT).
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Inter-Organellar Nucleic Acid Communication by a Mitochondrial tRNA Regulates Nuclear Transcription
… dependent transcriptional repression of glutaminase diverted glutamine towards the synthesis and maintenance of nucleotide pools, and enhanced cancer cell growth. These findings uncover nucleic-acid mediated communication between two organelles and the existence of a machinery for nuclear …
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Antioxidant Programs Are Essential for Enzalutamide-Resistant Prostate Cancer
… metabolism with either a small-molecule glutaminase inhibitor or genetic knockout blocked this antioxidant pathway, further enhanced ROS levels, and blocked the growth of enzalutamide-resistant PCa. The critical role of the compensatory antioxidant pathways in maintaining …
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The Role of Glutamine and Mevalonate Pathway In Affecting The Stemness and Viability of Glioblastoma Stem Cells
… treatment of the cells with Compound 968, a glutaminase inhibitor, depleted the cells from the CD133 marker, slowed proliferation and limited growth on soft agar. Moreover, GBM stem cells showed an increase in the expression of genes related to the mevalonate pathway. We also found that …
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Investigating the interdependence of human BH3-only protein Noxa and glutamine in activated CD8+ T cells.
… Noxa protein induction, but inhibition of glutaminase does not. We show that glutamine promotes the translation of the Noxa protein through the mTOR pathway by facilitating leucine influx. Conversely, the entry of glutamine carbons into the TCA cycle following co-stimulation requires Noxa. …
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