Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"fluorescent microscope"”.
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Scalable Chemical Tool for Analyzing Brain: Electron Microscope-Like Images with Fluorescent Microscope
… be achieved with FM (i.e., conventional light microscope), allowing anyone to identify and localize biomolecules in the detailed ultrastructural context of neural circuits. In this study, we report a novel membrane probe and modified expansion microscopy (ExM) protocol aimed at achieving …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… Nfa1 protein in N. fowleri was observed by immunofluorescent assay using monoclonal antibodies. METHODS: Six-week-old female BALB/c mice were immunized with a purified recombinant His-tag fusion Nfa1 protein. Cell lines producing McAbs against the recombinant His-tag fusion Nfa1 protein were …
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Development and Evaluation of a Potentially Wearable Device for Circulating Cell Monitoring
… a specialized optical system similar to a fluorescent microscope, which optimizes the signal- to-noise ratio by maximizing cellular fluorescence and minimizing background interference. Experimental determination of the limit of detection (LOD) for the SiPM and device establishes their …
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Immune Blocking of Fluorescent Antibody Staining of Streptococcus Group A Compared with Anti-streptolysin O
<p>The fluorescent antibody blocking antibody (FABA) test has been discribed and compared with the conventional antistreptolysin 0 antibody (ASOA) test in the detection of Streptococcus pyogenes group A antibodies in sera samples.</p> <p>A slide inhibition test was used to detect the presence of …
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Effects of long-term hormone treatment on cognitive behavior and the structure of the medial prefrontal cortex during aging in female rats
… sections using Axiovision (Zeiss) on a fluorescent microscope and fiber densities were quantified. Behavioral results found that animals receiving estrogen in combination with MPA acquired the t-maze faster than no replacement animals, but there were no differences in performance on the …
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METFORMIN, CLIC1 AND TRANSCRANIAL STIMULATION: THE BASIS OF A NEW TARGETED THERAPY AGAINST GLIOBLASTOMA RELAPSE
… GSCs were transfected to encode a green-fluorescent chloride sensor that can be monitored in living cells under fluorescent microscope. We found that chloride flux increases upon EMF stimulation in wild-type GSCs while the increase is way lower in cells Clic1-/-, suggesting that CLIC1 …
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Involvement of endothelial cells and macrophages in mycobacterial infections
… its uptake by endothelial cells. Data from the fluorescent microscope showed the association of BCG-GFP signal with endothelial cells detected on the FACS caliber. Analysis by confocal microscopy confirmed internalisation of endothelial cells by mycobacteria. Endothelial cells were further …
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Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma
… system, and further detected using the multifluorescent markers from 16 OC patients. Patients were found to have heterogeneous CTCs, with 113/157 (72%) cells positive for CK/EpCAM (epithelial marker), 58/157 (37%) positive for vimentin (mesenchymal marker), and 17/157 (11%) positive for both …
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Formulation of non-ionic surfactant vesicles for therapeutic delivery of siRNA in cancer treatment
… had high siRNA encapsulation efficiency (~90%). Fluorescent microscope and flow cytometry studies on A549 cells, using fluorescent labelled negative control siRNA loaded in all the NISV formulations tested, indicated high cellular uptake by the cells.;These uptake results were confirmed with …
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Development of an Injectable Ablative Therapy for Resource-Limited Settings: Applications in Tumor Ablation
… the infusion, sectioning it, and imaging with a fluorescent microscope. Since the infusion pressure increases with rate and volume, this finding motivates the use of low rates and volumes when possible to improve localization. For low-volume infusions in which the pressure remained below the …