Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 275 for “"expression level"”.
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The regulation of expression level in NeuroD/BETA2 by PKA
… regulated by phosphorylation at post-translation level. For searching effect of phosphorylation of NeuroD/BETA2, We found kinase and phosphoryation site of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 …
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Point-of-care microfluidic assays for measuring expression level of antigen on blood cells
Elevated expression of a membrane protein CD64 on neutrophils is linked with the onset of sepsis, a life-threatening syndrome that contributes to millions of deaths annually worldwide. The survival rate of septic patients falls rapidly every hour the appropriate medication is delayed. Therefore, …
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Rational Engineering of Expression Level of Multi-Gene Systems encoding Natural Product Biosynthesis in Streptomyces
… novel compounds. However, cloning and controlled expression of these large BGCs in a heterologous host require tedious optimization on a case-by-case basis. This dissertation presents a synthetic biology platform to rapidly reconstitute BGCs by refactoring and physically piecing DNA fragments …
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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and Human corneal endothelial cells
… diseases in human. Aim: To characterize relative expression levels of SLC4 members in mouse and human corneal endothelium, using qRT-PCR, to identify further important members besides SLC4A11 and SLC4A4, and to explore how close to the base line levels the gene expression levels remain after cells …
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Attachment Ability and Melanoma Inhibitory Activity mRNA Expression Level Changes in Murine B16-F10 Melanoma Cells Post Nanosecond Electric Pulses
… and reverse transcribed into cDNA. The MIA mRNA expression levels were measured using the cDNA temple via quantitative real-time PCR, with β–actin as the internal control. The experiment was repeated three times (n=3). The results show that a single pulse did not affect the cell attachment …
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Correlating antisense RNA performance with thermodynamic calculations
… gene down-regulation at the post-transcriptional level. In this study, the major purpose is to find a correlation between the expression level and minimum free energy to enable the design of specific asRNA fragments. The thermodynamics of asRNA and mRNA hybridization were computed based on the …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… ERK pathway, NF-κB pathway, and GRK-2. RKIP expression level is reported to be critical in the differentiation process and the pathogenesis of many different cancers and inflammatory diseases. However, the understanding of how RKIP expression level is regulated is poor. In this thesis, I …
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Degradational and Transcriptional Investigations of the RAF Kinase Inhibitory Protein
… ERK pathway, NF-κB pathway, and GRK-2. RKIP expression level is reported to be critical in the differentiation process and the pathogenesis of many different cancers and inflammatory diseases. However, the understanding of how RKIP expression level is regulated is poor. In this thesis, I …
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One- and Two -Photon States for Quantum Information
The expression of the transgene gus was studied in all 40 callus lines, 12 regenerated T0 plants and the T1 and T2 progenies of 5 lines. The isogenic SC lines had an average expression level based on the activity of beta-glucuronidase of 158 +/- 9 units/mg protein (mean +/- SEM; n=3; variance …
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Regulation of cancer progression by hepatitis B virus X protein (HBx) and HBxAP/RSF1
… 6 7. Statistics 6 C. RESULTS 7 1. The protein levels of HBx are decreased by catalase and MnSOD 7 2. Catalase downregulates HBx protein levels at the translational level 7 3. Cysteine residues of HBx are essential to maintain protein stability . 9 4. Cysteine null mutant of HBx is insufficient …
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THE STUDY OF CD24 AS A PREDICTIVE INDICATOR IN CISPLATIN TREATMENT RESPONSE OF HEAD AND NECK CANCER
… to platinum-based therapy. In this regard, CD24 expression level appears to be a significant molecular phenotype of cisplatin resistance in laryngeal carcinoma. This study demonstrates that CD24 expression level in HNSCC has a linear relationship with cisplatin resistance, and it affects the …
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The Development of a Thermodynamic Model for Antisense RNA Design and an Electro-transformation Protocol to Introduce Auxotrophic Genes for Enhancing Eicosapentaenoic Acid Yield from Pythium irregulare
… new asRNA constructs capable of fine-tuning gene expression knockdown. The asRNA technology is now identified as an effective and specific method for regulating microbial gene expression at the posttranscriptional level. This is done by targeting mRNA molecules. Although the study of regulation by …
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Mechanism on Elongated Mitochondria Formation during Iorn Chelation-Induced Senescent Arrest
… old human diploid fibroblast. Next, we examined expression levels of Drp1 and Mfn proteins, which are known as molecules directly regulating mitochondrial fission and fusion process, respectively. The total cellular expression levels of Drp1 was not significantly changed or decreased, showing …
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THE ROLE OF PIAS PROTEINS IN THE SUMOYLATION OF 5-HT1A RECEPTORS
… and various PIAS constructs showed different expression levels. PIASxα significantly increased the SUMOylated 5-HT1A Rs compared to other PIAS proteins. In the second study, I tested the hypothesis that the treatment of rats with 8-OH-DPAT and/or EB increased expression of PIASxα resulting in …
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
… gene. Northern blot analysis revealed that the expression level of the DdRPA1 was constant throughout differentiation and the size of mRNA is the same at all stages, corresponding to a 81 kDa protein. Thus, it seems that the size change between the 62 kDa and 81 kDa is probably due to …
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Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
… tissues to characterize patterns of gene expression across single cells, transcriptome-wide and in a cell-type-specific manner (Part 1). Additionally, we characterized single-cell RNA sequencing methods as a resource for experimental design and data analysis (Part 2). Part 1: …
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Characterizing resistance of Erysiphe necator to fungicides belonging to the quinone outside inhibitors and demethylation inhibitors
… isolate with QoI resistance but an undetectable level of the major QoI mutation was shown to be heteroplasmic -- resistant strains could be selected from this isolate. DMI resistance mechanisms in E. necator included the Y136F mutation in CYP51 and cyp51 over-expression. The first mechanism was …
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In situ studies of sugar metabolism in Ricinus communis L. and Saccharum officinarum L.
… Northern blot analysis indicated that the RcSPP1 expression level of the germinating endosperm was very similar from day 2 to day 6. The expression of nsLTPc1 is cotyledon-specific. It is also confirmed by in situ hybridization. The results of nsLTPc1 in situ hybridization indicate that the …
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Plant-based Multicomponent Vaccines against Enteric Diseases
… assay (ELISA). The CTB-NSP4 protein expression level was found to be approximately 0.01% of total soluble plant protein (TSP) and the CFA/I-CTA2 protein expression level was about 0.002% of TSP. Assembly of the CFA/I-CTA2 subunit into the CTBNSP4 pentamer was detected by immunoblot …
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Mechanism of tissue transglutaminase upregulation and its role in ovarian cancer metastasis
… is secreted in the OC milieu and regulates the expression and function of TG2 primarily through the canonical Smad signaling pathway. Increased TG2 expression level correlates with a mesenchymal phenotype of OC cells, suggesting that TGF-β1 induced TG2 promotes epithelial-to-mesenchymal …
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