Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 164 for “"double-stranded DNA"”.
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… the aetiology remains largely unanswered. Anti-dsDNA antibodies have been found intrathecally and bordering lesions in multiple sclerosis patients and in view of their known pathogenity in lupus nephritis the aim of this project was to further investigate their role in multiple sclerosis. Using …
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Exogenously-introduced Homing Endonucleases Catalyze Double-stranded DNA Breaks in Aedes aegypti
… are selfish genetic elements which introduce double-stranded DNA (dsDNA) breaks in a sequence-specific manner. In this study, we aimed to validate a somatic assay to detect recombinant homing endonuclease (rHE)-induced dsDNA breaks in both cultured cells and adult female Ae. aegypti. While the …
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Methods and applications of using DNA-cleaving DNAzymes to cleave double-stranded DNA
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Mechanistic studies of bleomycin-mediated double-stranded DNA cleavage and structural studies of DNA containing normal and 4'-oxidized abasic sites
… to examine the role of partial intercalation in double-stranded (ds) DNA cleavage mediated by a single bleomycin (BLM), a bulky group ([-cyclodextrin) was chemically attached to the polyamine tail of BLM A5 to prevent the intercalation of the bithiazole tail. The ability of this analog (CD-BLM) …
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Assessing fetal topoisomerase IIα as a mechanism of in utero benzene toxicity in generating double-stranded DNA damage in CD-1 mice
… (BQ) specifically inhibits the critical DNA repair enzyme topoisomerase IIα (topo IIα) and is hypothesized to initiate leukemias through the generation of double stranded DNA (dsDNA) breaks in hematopoietic cells. My research examines the role of topo IIα as a target of benzene toxicity …
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W-reactivation (Inducible "SOS" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12
… is remarkably deficient in inducible "SOS" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and …
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Computational studies of semiflexible polymer translocation in nanopore systems
The translocation of double stranded DNA (dsDNA) through nanopore systems is a field with rich physics and many promising technological applications. Double stranded DNA is not fully flexible, and the translocation dynamics of semiflexible molecules are not very well understood. In this thesis, in …
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Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences
… chromosome that break in response to perturbed DNA replication). We previously showed that ITSs in yeast stimulated point mutations in DNA sequences adjacent to the ITS as well as several types of chromosomal rearrangements. The major class of these rearrangements was the terminal inversion, …
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An Approach to Analyzing and Predicting Force-extension Curves of Nucleic Acids
… curves of nucleic acids such as long double-stranded deoxyribonucleic acids (DNA) and ribonucleic acids (RNA). The dissertation comprises two parts. In the first part, we propose an approach to help analyze polymer force-extension curves that exhibit a distinct plateau region. When …
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Mitigating security issues in the evolving DNA synthesis industry
DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, construction, and sale of synthetic DNA. The tools and methods of synthesis …
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Isolation and characterization of bovine parvovirus DNA
… through a sucrose-CsCl step-gradient. The virion DNA was released from the capsids by alkali treatment. Upon chromatography on hydroxyapatite, two peaks of radioactivity were consistently observed. Peak I eluted at a sodium phosphate concentration of 0.17 M, intermediate between single- and …
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E1B and E4 adenoviral oncoproteins modulate cellular responses to DNA damage
… infection inundates the host cell with linear, double-stranded DNA molecules and an abundance of single-stranded DNA. The cellular responses of cell death and cell cycle arrest to this apparent profusion of damaged DNA are antagonized by overlapping activities encoded in the adenoviral E1B and …
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The TREX1 3' Exonuclease and Autoimmune Disease: Structural and Biochemical Analysis of Disease Mutants Involved in Autoimmune Dysfunction
The homodimeric TREX1 protein is a member of the DnaQ family of exonucleases and catalyzes the major 3′ exonuclease activity detected in mammalian cell extracts. Mutations within the Trex1 gene are the underlying cause of multiple autoimmune diseases including Aicardi-Goutières syndrome (AGS), …
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RAD51-MEDIATED ABASIC SITES PROTECTION PREVENTS REPLICATION FORK COLLAPSE.
Genomic DNA is continuously subjected to damage caused by endogenous and environmental agents. This damage, produces as a result DNA lesions, among which abasic (AP) sites are the most prevalent in genomic DNA, occurring at an estimated rate of 10,000 to 20,000 per cell per day. Under normal …
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Nanoparticle probes for the study of biomolecular interactions
… nanoparticles for the detection of single and double stranded DNA sequences. The oligonucleotides are modified to incorporate locked nucleic acid (LNA) ribose sugars, affording highly advantageous affinity and selectivity properties. Hybridisation of the probes with the target sequence was …
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CBK Bacteriophage Genome Annotation and Organization
… of the CBK genome. Bacteriophage CbK is a double-stranded DNA phage with an extremely large genome size of 203,242 base pairs and a 66.13% GC content. Of the 303 predicted coding sequences, 60 sequences had significant similarity with the other bacterial and phage proteins, with 20 of them …
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Understanding the mechanism of antiretroviral nucleoside analogs as inhibitors of Epstein-Barr virus lytic DNA replication
Epstein-Barr virus (EBV) is a human B-cell tropic double-stranded DNA y-herpesvirus. To date, there are no antiviral agents proven to be clinically effective in the treatment of EBV infection or EBV-associated diseases. In the experiments contained in this thesis, we define the ability of …
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Methods and Applications of Computational Design in Multiple States
… enzyme to remove allosteric dependence on double-stranded DNA for activity. In both cases I started by applying simple computational methods to identify mutations that are likely to shift the equilibrium from an undesired state to a desired state. I then incorporated bioinformatics data to …
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