Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"deubiquitinase"”.
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Discovery and characterization of a novel Deubiquitinase ZUP1
Deubiquitinases (DUBs) regulate the post-translational protein modification<br/>ubiquitylation, which is involved in various cellular processes including protein<br/>degradation, an array of signalling pathways and the DNA damage response.<br/>The recent discovery of a fifth family of cysteine …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
<p>Ubiquitin carboxyl-terminal hydrolase 37, or UCH37, is a deubiquitinating enzyme associated with the 26S proteasome, the primary protein degradation machinery in eukaryotic cells. UCH37 is responsible for the disassembly of polymeric ubiquitin chains, or polyubiquitin, which have been ligated …
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Expressão e análise de atividade enzimática da deubiquitinase USP2a recombinante
… perante diferentes substratos. A enzima deubiquitinase USP2a está envolvida com diversos processos celulares, e defeitos em sua expressão estão presentes em diversas patologias, como o hepatocarcinoma e câncer de próstata, sendo vista como um possível alvo terapêutico. Entretanto, até o …
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Quantitative Deubiquitinase (DUB) Interactomics to Dissect the Biology of Cancer Associated DUBs
… is carried out by the class of enzymes called deubiquitinases (DUBs). These DUBs have vital roles in the regulation of protein ubiquitination and have a profound influence on the fate of proteins and their activity. Dysregulation of DUBs has been associated with the development of many human …
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Inhibition of Deubiquitinase Activity and Ubiquitination of Jak2 Blocks Cytokine Signaling and Induces Tumor Cell Apoptosis
… DUB inhibitor. It specifically inhibits the deubiquitinase activity of USP9x, USP5, USP14 and UCH37. WP1130 mediated inhibition of tumor-associated DUBs resulted in down-regulation of anti-apoptotic and up-regulation of pro-apoptotic proteins, such as MCL-1 and p53 respectively. Our results …
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Deubiquitinating Enzymes Promote Cancer Progression and Metastasis Via Regulating Protein Stability
<p>Deubiquitinating enzymes (DUBs, also called deubiquitinases) are enzymes that remove monoubiquitin or polyubiquitin chains from target proteins. DUBs have critical roles in cell homeostasis and signal transduction, as they regulate protein degradation, subcellular localization, and …
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Multifunctional Coronavirus Papain-Like Proteases as Targets for Antiviral Therapeutics and Vaccines
… is a multifunctional enzyme with protease, deubiquitinase, interferon antagonism and deISGylating activities. Despite low sequence identity, this multifunctionality of PLPs seems to be conserved among many coronaviruses. I demonstrated protease and deubiquitinase activity of PLP2s from …
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Identifying Novel Interactions Between Human Ubiquitin-Specific Protease 7 (USP7) and Histone Methylating/Demethylating Proteins
The deubiquitinase, Ubiquitin-Specific Protease 7 (USP7) is an indispensable protein responsible for regulating appropriate turnover of proteins involved in numerous signaling pathways and biological processes including immune response, DNA damage response, DNA replication, epigenetics and cell …
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Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… experiments, in which we observe VCPIP1, a deubiquitinase, bound to two distinct sites on VCP. Using cryo-electron microscopy, we determined structures of VCP-VCPIP1 complexes in the absence of added nucleotide or in the presence of an ATP analog and observe VCPIP1's catalytic site below …
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OTULIN's Novel Regulatory Mechanisms in Genotoxic and Inflammatory NF-kB Signaling
… transduction upon genotoxic stress. OTU deubiquitinase with linear linkage specificity (OTULIN) is known to cleave linear ubiquitin chains exclusively. The purpose of this dissertation is to investigate whether OTULIN can counteract LUBAC-mediated NF-</p>
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LNCFOXP3 CONTROLS CELL IDENTITY AND FUNCTION OF HUMAN REGULATORY T CELLS
… by mass spectrometry. Analysis revealed USP7, a deubiquitinase known to prevent FOXP3 from proteasome-mediated degradation. By exploiting both primary Treg cells and ectopic expression of lncFOXP3 and FOXP3 in a 293T model system, we found that lncFOXP3 contributes specifically to FOXP3 protein …
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The emerging roles of BAP1 and cancer stem cells in Malignant Mesothelioma
… protein 1) BAP1 gene. BAP1 is a deubiquitinase and a tumour suppressor, involved in cell cycle, apoptosis, epigenetic modifications and DNA damage response. Herein, it has been demonstrated that BAP1 mutated and silenced MMe cells are more resistant to a DNA damage drug, …
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Mitochondrial regulation of CD8+ T cell cytotoxicity
… shown to display altered CTL killing. USP30 is a deubiquitinase localised on the outer mitochondrial membrane and on the peroxisomal membrane, where it counteracts mitophagy and pexophagy by cleaving ubiquitin chains from target proteins. Here I characterise T cell motility, signalling, energy …
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Global and chromosome-specific consequences of aneuploidy in Saccharomyces cerevisiae
… cell biology. Additionally, we identify a deubiquitinase, Ubp3, whose deletion broadly impairs aneuploid yeast cell fitness via both karyotype-dependent and karyotype-independent mechanisms. Deletion of UBP3 severely exacerbates proteotoxicity in aneuploid yeast and impairs proteasome …
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Investigation of Huntingtin’s Role in DNA Repair and Transcription
… the DNA repair activities of PNKP, and deubiquitinase activity of Atxn3. This finding is supported by accumulation of DNA damage in transcriptionally active regions of the genome, compared to silenced regions, in HD cell and animal models. Furthermore, mHTT induced cell toxicity is …
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Identification and Optimization of Small Molecule Binders for ALK2, USP5, and DCAF1
… exhibited by the reference compound. For USP5, a deubiquitinase overexpressed in most cancers, we conducted structure-activity relationship (SAR) studies to optimize UBTR008295a. Our optimization studies led us to the discovery of compound 22, which exhibited single-digit micromolar affinity for …
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Ubiquitin Mediated Regulation of NF-kappa B Signaling
… evidence that CYLD functions as a K63 deubiquitinase to attenuate NF-κB signaling.
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Phosphatase Regulation of Mechanical Stress and Aging in C. elegans
… feedback loop and represses via inhibition of a deubiquitinase, MATH-33, affecting proteasomal degradation. Thus, I describe an uncharacterized stress response pathway in C. elegans and identify transcriptional and post-translational components comprising a feedback loop on Jun kinase and …
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Locus-specific proteomics identifies novel repressors of Epstein-Barr virus reactivation
… identified massive upregulation of the human deubiquitinase USP17. USP17 upregulation was driven by EBV lytic proteins and overexpression of USP17 was associated with deubiquitination of H2AK119Ub. The complexity of the USP17 gene locus, comprising at least 32 copies, made its depletion very …
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Proviral Roles of Ebola virus VP35 Ubiquitination
… of unanchored ubiquitin chains, using the deubiquitinase isopeptidase T, impedes VP35’s non-covalent binding to ubiquitin and stunts EBOV’s polymerase activity. Finally, we also found that TRIM25, TRIM6’s relative, facilitates ubiquitin ligation onto VP35 and enhances VP35’s non-covalent …
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