Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 340 for “"cell-free"”.
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Cell-Free Formation of RNA Granules
… RNA localization is a mechanism by which a cell can spatially and temporally regulate the translation of RNAs. This mechanism is essential for many developmental processes such as germ cell formation in Drosophila embryos, as well as establishment of cell polarity and synaptic plasticity in …
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Know Thy Cell-Free DNA: Early Detection of Microsatellite Instability Using Ultra-Low-Pass Cell-Free DNA Sequences
… than other genomic locations, and since cancer cells undergo many more cell divisions, microsatellites are useful for distinguishing tumor DNA from normal (non-cancerous) DNA. Microsatellite instability (MSI) arises as a result of mismatch repair deficiency (MMRD), wherein a patient loses …
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Cell-Free Dna Sequencing In Multiple Myeloma
<p>Multiple myeloma (MM) is an incurable plasma cell dyscrasia. Recent advances in MM therapy, including CAR-T therapy, have increased survival and shown the value of assessing treatment response with great sensitivity, both in acute and long-term settings. Cell-free DNA, DNA fragments which are …
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Tools for RNA and cell-free synthetic biology
… RNA synthetic biology and synthetic minimal cells are areas where design is limited by the availability of tools to observe, program, and manipulate the systems in question. In this work I present expanded toolsets to achieve these goals. The ability to monitor and perturb RNAs in living …
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Cell-free formation of protease-resistant prion protein
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1996.
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Genetically encoded division machinery for cell free synthetic biology
The de novo construction of cellular life requires, in part, the assembly of components that confer the ability to replicate. Herein we describe efforts to reconstitute parts of the Escherichia coli cell division machinery inside of water-in-oil emulsion compartments and synthetic phospholipid …
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The Democratization and Development of Cell-Free Protein Synthesis
<p>Cell-free protein synthesis (CFPS) using crude lysates has developed into a robust platform technology over the last 60 years to express numerous types of recombinant proteins. The open-nature, elimination of reliance on cell viability, and focus of all energy towards production of the protein …
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Cell-free synthetic biology for affordable, on-demand diagnostics
… diseases, and inform public health decisions. Cell-free synthetic biology has shown promise as a portable, affordable technology to detect biomolecules like nucleic acids. In this thesis, I present several advancements to cell-free synthetic biology diagnostics that enable new application …
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Cell-free expression systems for the construction of artificial cells
Cell-free expression systems are widely used to synthesize proteins for subsequent further characterization, to manufacture potentially useful commercial end products, and to construct cellular mimics in the laboratory. The first part of the thesis explores the feasibility of preparing two of the …
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Terminal Oxidation in Cell-Free Extracts of Plant Pathogenic Fungi
Made available in DSpace on 2014-12-05T20:30:13Z (GMT). No. of bitstreams: 1 6200589.pdf: 3809775 bytes, checksum: a3d652e7725054bb711c77740371eb2e (MD5) Previous issue date: 1961
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Cell-free freeze-dried synthetic biology for wearable biotechnology applications
Synthetic biology aims to develop modular genetic networks for computation, sensing, and control of biological systems, holding great promise for next-generation biosensing platforms. Similarly, advances in material sciences have allowed for the design of substrates and textiles engineered to …
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Expanding the accuracy, resolution, and breadth of cell-free DNA investigation
When cells die, they don’t simply vanish without a trace. Instead, they leave behind fingerprints of their genetic and epigenetic identities in the form of cell-free DNA (cfDNA), or the scant amount of highly fragmented DNA circulating in human plasma. As the detritus of apoptotic and necrotic cell …
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Requirements for Cell-Free Cyanide Oxidation by Pseudomonas Fluorescens NCIMB 11764
… shown to occur indirectly by the consumption of free cyanide arising from the cyanohydrins via chemical dissociation. Thus, free cyanide remains the substrate, for which the enzyme displays a remarkably high affinity (Kmapp,4 mM). A model for cyanide utilization is therefore envisioned in which …
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ToxIN-mediated resistance to and cell-free production of Salmonella bacteriophages
… of FelixO1 using an Escherichia coli-based cell-free expression system, demonstrating for the first time that a Salmonella phage can be produced in this manner. Taken together, this work illustrates that ToxIN may be of concern for food applications of phages while also establishing an …
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Cell-free platform for the functional characterization of intracellular ion transporters
… functional readout methods. Historically, cell-based protein production methods have dominated the field of membrane protein studies; but now, cell-free protein synthesis (CFPS) approaches are beginning to both complement and rival traditional expression methods. Regarding readout methods, …
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Cell free synthesis of ferritin using the Modified Reticulocyte Lysate System.
Thesis: M.S., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1978
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Biological, Clinical, and Functional Implications of Cell-Free DNA Topology in Cancer
Cells can release their DNA into the extracellular space and circulation by active mechanisms or as a product of cell death. In oncology, this cell-free DNA (cfDNA) has emerged as a promising source of cancer biomarkers because it contains actionable information about a patient’s disease and can be …
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Studies on the Cell-Free Protein-Synthesizing System of Aging Rat Liver
Made available in DSpace on 2014-12-11T22:22:26Z (GMT). No. of bitstreams: 1 7514149.pdf: 2979333 bytes, checksum: 2d78eef0462aec991a637c3128a2f1c8 (MD5) Previous issue date: 1975
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