Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"cell isolation"”.
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Dietary regulation of myelopoiesis and stromal cell isolation
… chloride did not alter monocyte, granulocyte, T cell, B cell, or NK cell distributions in blood. In spleen, 10% sodium chloride did reduce the presence of NK cells but had minimal effect on other cell populations. In bone marrow, 10% sodium chloride increased the presence of T cells and both …
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Ex vivo perfusion optimization of donor liver grafts for transplantation and cell isolation
… of high quality hepatocytes in the fields of cell transplantation, pharmacotoxicology, tissue engineering, and bioartificial assist devices. The scarcity of viable hepatocytes necessitates the use of suboptimal sources including damaged donor organs that are not transplantable. Many of these …
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Microfluidic cancer cell isolation system using magnetic nanoparticle hybrids and advanced interdigitated electrode technology
… in improving magnetic sensing technologies for cell isolation systems. These technologies, through the popular biomarkers, i.e. DNA, RNA, proteins, antibodies, have already been implemented in clinical diagnosis and/or prognosis.An example of a successful commercial product is Invitrogen’s …
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Engineering and optimizing physiological cell isolation via the secondary anchor targeted cell release system
… and differences in biomarker expression on cells. Quantification of these biomarkers, then, would allow a methodology for designing personalized treatments and regimens that the patients would no longer be resistant to. However, techniques designed to purify or isolate cells to quantify …
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Lab on a chip rare cell isolation platform with dielectrophoretic smart sample focusing, automated whole cell tracking analysis script, and a bioinspired on-chip electroactive polymer micropump
… Contactless DEP (cDEP) is a recently developed cell sorting and isolation technique that uses the DEP force by capacitavely coupling the electrodes across the channel. The cDEP platform sorts cells based on intrinsic biophysical properties, is inexpensive, maintains a sterile environment by …
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Combining optical and hydrodynamic forces for single cell characterization, isolation and delivery
With the existence and emerging importance of cellular heterogeneity there has been a push in the microbiology community for new tools to study at the individual cells. To meet the need to identify, characterize and isolate single cells of interest from bulk samples, precise and directed forces on …
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Use of gene expression to characterize heterogeneous liver cell populations
Non-parenchymal cells (NPC's) are integral to recreate the native hepatic microenvironment and necessary to maintain in vivo liver function. A variety of in vitro culture systems have been developed to address different aspects of liver physiology and architecture in order to recreate the …
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Technologies for the isolation of circulating tumor cells
Metastasis, the spread and growth of tumor cells from the primary site to distant organs, is arguably the most devastating and deadly attribute of cancer, and is ultimately responsible for 90% of cancer-related deaths. Circulating tumor cells (CTCs) are exceedingly rare cells found in the whole …
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Isolation and characterisation of canine osteosarcoma cells to investigate mechanisms of chemoresistance
… the protocol for isolating primary canine OSA cells and to compare chemoresistance and the role of autophagy in these cells with an immortalised canine osteosarcoma cell line (OSCA) and canine osteoblasts isolated from trabecular bone. Methods: OSA tumour biopsies were isolated during limb …
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Imprinted Magnetic Traps for Study on Particle Fluctuation, Ordering and Microfluidic Applications
… of functional materials and marker-based cell isolation. During my pursuit of Ph.D. in Physics in Prof. R. Sooryakumar’s lab, I have worked on magnetic trapping platforms based on ferromagnetic thin-film patterns imprinted on a substrate. These design-based platforms serve as excellent …
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Development Of Single-Cell Transcriptomic & Epigenomic Sequencing Technologies To Assess Cell-Type-Specific Gene Regulatory Programs In Mammalian Brains
Recent efforts to develop single-cell technologies have played a pivotal role in our ability to gain a high-resolution understanding of how tissue heterogeneity impacts human health, development, and disease. These technologies have become so powerful and popular in such a short period of time, and …
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Lung tissue engineering : in vitro synthesis of lung tissue from neonatal and fetal rat lung cells cultured in a three-dimensional collagen matrix
… gestation) and neonatal (2 days old) rat lung cells were grown in a collagen-glycosaminoglycan scaffold. This project employed a collagen-GAG scaffold specifically developed for tissue engineering and investigated the effect of this substratum on the formation of lung histotypic structures in …
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Exploiting Clausius-Mossotti Factor to Isolate Stages of Human Breast Cancer Cells: Theory and Experiment
… of contactless dielectrophoresis (cDEP) for isolation of breast cancer cell stages. The ability to selectively concentrate breast tumor cells from a non-transformed or normal cell population is the key to successfully detecting tumors at an early stage of growth and treating transformed cells …
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Sessile Droplet Microfluidic Platforms for Improvements in Mass Spectrometry Sample Preparation
… explored as an open surface platform for single cell isolation and analysis. Using silica particle suspensions, microspot fabrication was optimized for single particle occupancy and average particles per microspot.
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Secondary anchor targeted cell release system
… probe individual heterogeneities in patient’s cell receptor expression limits advancement in personalized medicine. These individual differences in receptor quantities can give rise to both intrinsic and acquired resistances to therapeutics, which result in reduced treatment efficacy. In …
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In vitro comparison of equine tendon- and bone marrow-derived cells expanded with FGF-2 prior to culturing with tendon matrix and IGF-I
… of equine tendon- and bone marrow-derived cells prior to culture with autogenous acellular tendon matrix and insulin-like growth factor-I (IGF-I). Progenitor cells were isolated from six young adult horses, expanded in monolayers with FGF-2, and cultured with autogenous acellular pulverized …
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Microfluidic technology for cellular analysis and molecular biotechnology
… for biological analysis of a low number of cells. The technological advances in microfluidics have allowed both forming of valves, mixers and pumps and integrating of optic and electronic components into microfluidic devices to construct complete and functional systems. In this dissertation, …
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Utilizing Toxoplasma gondii To Understand Neuron-Specific Neuroinflammation
… decreased expression of cytokine and immune cell receptors, as well as an inability to intracellularly clear the parasite. Evidence in the last five years has shown that neurons are capable of intracellular clearance, often in unusual ways, and are likely able to interact with cytotoxic …
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Regulation of Hematopoietic Stem Cells
… is about the regulation of hematopoietic stem cells (HSCs) that represent rare cells residing in the bone marrow (BM) of adults. They are multipotent cells that have the capacity to differentiate to all mature cells of the blood system and have the capacity to self-renew, i.e. generate new …
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The role of extracellular matrix in modulation of myocyte Ca2+ signalling and contraction in the isolated rat heart
Traditionally the extracellular matrix (ECM) in the heart has been thought to provide mechanical scaffolding for the contracting myocytes. However, it was observed that the amplitude of the intracellular Ca2+ transients that underlie contraction was reduced in isolated myocytes in comparison to …
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