Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"binding constants"”.
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Biochemical and genetic analysis of nucleic acid-protein interactions
… coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific binding …
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Development of an Enzyme-Linked ImmunoSorbent Assay (ELISA) with Enhanced Sensitivity in a Nanofluidic System
… to evaluate the kinetics and equilibrium binding constants of biomolecules in nanofluidic channels. Binding events in the nanochannel were detected using electrical and fluorescence methods. We concluded that antibody-antigen binding constants in nanochannels were similar to experiments …
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Investigation of the effect of hydroxycitric acid on urinary calcium oxalate risk factors for kidney stone formation in artificial urine: theoretical modelling and in vitro crystallisation experiments
… involving the determination of thermodynamic binding constants for Ca, Mg and Zn-HCA complexes, theoretical modelling of HCA-complex formation in artificial urine and in vitro crystallisation experiments. A potentiometric analysis was conducted to determine thermodynamic binding constants. …
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The effect of inositol-hexakisphosphate (phytate) on urinary risk factors for calcium oxalate urolithiasis in South African population groups with different kidney stone risk profiles : theoretical modelling, in vitro crystallisation experiments and in vivo human studies
… were conducted to determine thermodynamic binding constants of soluble calcium-phytate complexes. Binding constants of seven complexes were identified. These were included in the data base of the Joint Experts Speciation System computer program to model the effect of phytate on the urinary …
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SENSORS BASED ON IMMOBILIZED FLUOROGENIC REAGENTS
… The response time is 1-2 minutes. Conditional binding constants for the immobilized morin-metal complexes are on the order of 10('3) to 10('4) for the pH range 3 to 5. The sensor extracts about 1% of the Al(III) or Be(II) in the sample when operating in the range of linear response and serves …
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Spectroscopic Studies of Chlorophyll a Complexes with Trinitro-Substituted Flourene Derivatives
… between the donor and acceptor. Ground state binding constants (Ka) of each system were also determined from the y-intercept of these plots. Fluorescence data were analyzed with a Stern-Volmer plot. The quenching constants (Ksv) were obtained from the slope of these plots. These results show …
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Lanthanide complexes as fluorescent indicators for neutral sugars and cancer biomarkers
… complex binds neutral sugars with apparent binding constants comparable to those of arylboronic acids. Interference from commonly occurring anions is minimal. The europium complex detects sialic acid-containing gangliosides at pH 7.0 over an asialoganglioside. This selectivity is attributed, …
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Characteristics and Performance of Novel Squarylium Dyes as Fluorescent Probes for the Analysis of Proteins and Viruses by CE-LIF
… labels to aid biomolecule detection. Noncovalent binding of dyes through hydrophobic, electrostatic, and other interactions with biological molecules can result in improved sensitivity by assays using capillary electrophoresis with laser induced fluorescence detection (CE-LIF). Results from such …
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Binding and transport of calcium by synthetic analogues of ionomycin
… the 13-diketone and the carboxyl groups. The binding constants of the analogues with calcium and magnesium in 80% Me0H-H20were determined to be in the order of 102 M-1 and 103 M-1 respectively using the pKa method. The binding constants of the analogues with magnesium were also determined by …
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A Break In Communication: The Synthesis, Characterization, DNA Binding and Photocleavage of a Novel Ruthenium Polypyridyl Complex Containing an Electronically Isolated Pyrene Group
… experiments including isothermal and competitive binding titrations in addition to the hydrodynamic technique of viscometry were used to show that [Ru(bpy)2bpy-py]2+ binds to DNA with a binding constant of 8 x 105 and 1x106 M-1 (binding constants calculated using isothermal and competitive binding …
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Towards interlocked structures based on H-bonded barbiturate complexes
… privileged position of Hamilton’s barbiturate binding system in supramolecular chemistry, this motif has never been used in generating interlocked structures, such as rotaxanes or catenanes. This thesis demonstrates the feasibility of such structures. A series of Hamilton-like receptors has …
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Studies on the interaction of surfactants and neutral cyclodextrins by capillary electrophoresis. Application to chiral analysis
… of the stoichiometry as well as of the binding constants of SDS-CDs complexes. The results of these investigations were compared to those obtained with a different technique, electronic paramagnetic resonance (EPR). The obtained results suggested that methylated CDs, in particular …
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The synthesis and investigation of the electronic properties of crown ether, [2]-catenane, and [2]-rotaxane architectures
… rotaxane, and catenane genres. The binding constants of thiophene and phenylene-ethynylene based crown ethers are determined via fluorescence quenching titrations, in an attempt to correlate structure with binding affinities. The insight obtained from the binding constant …
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On the origins of enzyme inhibitor selectivity and promiscuity: a case study of protein kinase binding to staurosporine
… find the relationship between the shapes of ATP binding sites and their affinities for inhibitors. My shape-based dendrogram shows clustering of the kinases based on similarity in shape. I investigate the pocket in terms of conservation of surrounding amino acids and atoms in order to identify …
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Interactions of cadmium selenide (CdSe) quantum dots with coagulant proteins extracted from Moringa oleifera seeds
… mechanisms. Using Stern-Volmer analysis, the binding constants (Kb), quenching constants (Kq) and number of binding sites (n) were determined. The thermodynamic potentials Δ𝐻𝜃(-321.3 kJmol-1); Δ𝑆𝜃( 156.0 JK-1mol-1) and Δ𝐺𝜃(-46.6 kJmol-1) were calculated and gave an indication that the …
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Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… plasmon resonance (SPR) was used to study the binding of exonuclease deficient E. coli polymerase I, Klenow fragment (KF), to DNA modified with AF or AAF at two locations: as a templating base or in the last formed base pair. KF binding to the modified DNA bases was also monitored to DNA that …
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MOLECULAR RECOGNITION PROPERTIES OF MOLECULAR CONTAINERS IN AQUEOUS SOLUTIONS
… able to bind CB[8] with femtomolar dissociation constants. It also shows that these ultratight binding complexes can be found in competition measurements with slightly weaker ternary complexes, thus reducing the number of measurements needed and the error of those measurements. Chapter 3 shows …
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Comparative analyses of aryl hydrocarbon receptor structure and function in marine mammals
… for expressed genes. The molecular sequences, binding constants, and transcriptional activities for North Atlantic right whale and humpback whale AHRs cDNAs were determined using in vitro and cell culture methods. Whale AHRs are capable of specifically binding dioxin and initiating …
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Computational Studies on Nucleic Acid-Ion Interactions in Droplets and Bulk Solution
… solution, free energy calculations examine the binding of individual sodium and potassium ions to guanine quadruplexes; the thesis reports the values of the Gibbs free energy and the binding constants of each cation. Additionally, the free energy calculations examine the effect of the guanine …
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Characterization and Synthesis of Cyclodextrin Inclusion Complexes and their Applications as Fluorescent Probes for Sensing Biomacromolecules
… This combination of functionality and guest binding ability makes CDs important scaffolds for the design of functional supramolecular systems. This thesis explored the interaction of heptakis-[6-deoxy-6-(2-aminoethylsulfanyl)]-β-cyclodextrin (1) with many hydrophobic guest molecules. The …
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