Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"UPLC-MS/MS"”.
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Biomarker analysis for APRT deficiency diagnosis using UPLC-MS/MS
… enzyme is absent, adenine is oxidized and forms 2,8-dihydroxyadenine (DHA). DHA is insoluble in urine and can precipitate and form kidney stones, which can cause acute and chronic kidney failure. The disease can be treated with the drugs allopurinol and febuxostat, which prevent the conversion …
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Mass Spectrometry Based Diagnostics for APRT Deficiency: A Validation of a UPLC-MS/MS Urinary Assay for Absolute Quantification of 2,8-dihydroxyadenine and Adenine
… í Adenosín Mónófosfat (AMP). Fjarvera APRT ensímsins leiðir til niðurbrots adeníns yfir í 2,8-díhýdroxíadenín (DHA) af ensíminu Xanþín oxídasa. DHA er torleyst við sýrustig þvags sem veldur því að það fellur út í umfrymi nýrnanna og safnast þar fyrir, sem getur valdið nýrnaskaða, myndun …
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Examining the Roles of Membrane Lipids and Secreted Bioactive Lipids on Immune System Cell Function: Studies on Mast Cells and Platelets
… liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods to address the challenge of quantifying mast cell-secreted bioactive lipids in complex cell cultures as well as understanding the roles that membrane phospholipids play in platelet signaling. With the exception of red blood …
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RILIEVO DI FATTORI XENOBIOTICI, CON PARTICOLARE RIFERIMENTO A NICOTINA E COTININA, IN CAMPIONI ISTOLOGICI APPARTENENTI A FETI E NEONATI DECEDUTI PER CAUSE NATURALI.
… for toxicological substances and then by ICP-MS analysis for 61 different metals. At last, all the 151 samples have been subjected to UPLC/MS-MS technique for the relief and dosage of nicotine and cotinine.
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The determination of veterinary antibiotics in live animals and animal products
… This thesis was undertaken to develop a UPLC-MS/MS method for the simultaneous analysis of the aminoglycoside and macrolide antibiotics. This is the first time that a combination method for the two classes of compound was developed. A Waters Acquity/ Premier XE system with a dual ESI/APCI …
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Stability of heroin metabolites and oxycodone in rat hair and liver during decomposition
… and buried in controlled burial microcosms. Concentrations of oxycodone and selected metabolites or the metabolites of heroin; 6-monoacetylmorphine and morphine, incorporated within rat hair and liver were measured before and during the decomposition process. Oxycodone was analysed in …
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Advanced systems for the rapid detection of anthelmintic drugs in food
… for purpose when its performance was compared to UPLC-MS/MS analyses of milk from cows treated with benzimidazole products. In bovine liver the LOD (32 μg kg-1) and the CCβ (50 μg kg-1) were determined and the analyte recovery was in the range 77-132%. All non-compliant samples were identified …
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Improving CNS Delivery of Genistein for the Treatment of Sanfilippo Syndrome
… MPS III. Towards our goals, two major specific aims were proposed: (1) To improve accumulation of genistein in brain and (2) To evaluate the efficacy of genistein formulation. Methods: Genistein nanosuspensions were prepared by wet milling technique and characterized in order to select the most …
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Vitamin D as a measure of health throughout the lifecycle
… Liquid Chromatography- Tandem Mass Spectrometry (UPLC-MS/MS) assay. A second aim was to examine the associations between 25OHD and a number of non-skeletal health outcomes across the lifecycle, and, thirdly and finally, to determine the dietary and supplement sources of vitamin D in two of these …
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Desarrollo y evaluación toxicológica de compuestos aliáceos para su aplicación en el ámbito agroalimentario
… para la detección de OSCs en piensos, utilizando UPLC-MS/MS. Además, se realizaron estudios de biotransformación del PTSO en microsomas, revelando la formación de metabolitos no tóxicos. En el estudio toxicocinético en ratas se reveló que el PTSO no fue absorbido hacia el plasma ni tejidos …
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Metabolism And Pharmacokinetics In The Development Of New Therapeutics For Cocaine And Opioid Abuse
… of CM304 in rats using a validated UPLC-MS/MS method. Mitragyna speciosa Korth is a tropical plant indigenous to Southeast Asia. The leaves of the plant have been used as a traditional medicine to treat cough, fever, diarrhea, pain, hypertension and morphine addicts. Mitragynine and …
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The Role of Cytochrome P450 2E1 in Hepatic Ischemia Reperfusion Injury and Reactive Oxygen Species Formation
<p>The mechanisms of hepatic ischemia/reperfusion (I/R) injury, which occurs during liver transplantation or surgery, are poorly understood. The central pathophysiological phenomenon of this injury is the formation of reactive oxygen species (ROS). Recent studies suggest that P450 enzymes may …
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
… hydroxylated metabolites of midazolam by UPLC-MS/MS. We also aim to analyze the protein levels of CYP3A in the rat brain mitochondria and microsomes by Western blot analysis.</p> <p>Microsome and mitochondria were prepared using different centrifugation methods, followed by purification of …
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Single dose pharmacokinetics of pimobendan in healthy horses
… liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and pharmacokinetic parameters determined by noncompartmental analysis. No significant differences were noted between formulations or sample site (P < 0.05). Concentrations in compounded formulations were 88%(S) and 90%(C) of label. For …
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Improving Non-nutritive Sweetener Study Design Methodology
… using ultra performance liquid chromatography (UPLC-MS/MS), which analyzes each sample for the presence of NNS and related metabolites. This method observed a range from very strong presence of NNS to not detectable, indicating that this biomarker could identify specific NNS consumption (n=9). …
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Aqueous humor concentration and prostaglandin E2 suppression efficacy of topically applied ophthalmic ketorolac 0.5% and diclofenac 0.1% solutions in dogs with cataract
… liquid chromatography-mass spectrometry (UPLC-MS/MS) and enzyme-linked immunoassay (ELISA) analysis, respectively. Results: Median aqueous humor drug concentrations were significantly higher in dogs treated with ketorolac 0.5% (1311.6 ng/mL) compared to those treated with diclofenac 0.1% …
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Gut Health Benefits of Natural and Alkali-Processed Cocoa (Theobroma cacao) with and without Inulin
… in the mitigation of IBD. This study aims to 1) investigate effects of alkali processing on gut health related bioactivity and phytochemical composition of cocoa and 2) evaluate potential additive benefits of combining cocoa and inulin. Polyphenolic and flavanol compounds in natural …
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Pharmacocinétique comparative des curcuminoïdes avec ou sans oméga-3 : une étude en chassé-croisé randomisée
… with a triple quadrupole mass spectrometer (UPLC-MS/MS). Participants mean age was 27.6 ± 6.0 years old and they had an average body mass index of 25.1 ± 3.8 kg/m2. Twenty-four hours after a single dose intake, the total plasma curcuminoids area under the curve (AUC) reached 166.8 ± 17.8 …
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Metabolic Stability And Pharmacokinetics In Lead Optimization Of Potential Anti-Psychostimulant Pharmacotherapies
… it also leads to several socioeconomic problems like healthcare expenditures, lost earnings and increase in drug-related crime. The discovery and development of potential pharmacotherapies to treat cocaine dependence has been a high priority for more than two decades but still there is US-FDA …
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