Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"UPLC-MS"”.
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Biomarker analysis for APRT deficiency diagnosis using UPLC-MS/MS
… enzyme is absent, adenine is oxidized and forms 2,8-dihydroxyadenine (DHA). DHA is insoluble in urine and can precipitate and form kidney stones, which can cause acute and chronic kidney failure. The disease can be treated with the drugs allopurinol and febuxostat, which prevent the conversion …
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Mass Spectrometry Based Diagnostics for APRT Deficiency: A Validation of a UPLC-MS/MS Urinary Assay for Absolute Quantification of 2,8-dihydroxyadenine and Adenine
… í Adenosín Mónófosfat (AMP). Fjarvera APRT ensímsins leiðir til niðurbrots adeníns yfir í 2,8-díhýdroxíadenín (DHA) af ensíminu Xanþín oxídasa. DHA er torleyst við sýrustig þvags sem veldur því að það fellur út í umfrymi nýrnanna og safnast þar fyrir, sem getur valdið nýrnaskaða, myndun …
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Examining the Roles of Membrane Lipids and Secreted Bioactive Lipids on Immune System Cell Function: Studies on Mast Cells and Platelets
… liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods to address the challenge of quantifying mast cell-secreted bioactive lipids in complex cell cultures as well as understanding the roles that membrane phospholipids play in platelet signaling. With the exception of red blood …
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Third Trimester Plasma Metabolites Associated with Gestational Diabetes: An Ancillary Analysis of the Healthy Beginnings Trial
… in the study. Plasma samples were analyzed using UPLC-MS with metabolomic assays for primary metabolomics, aminomics, and lipidomics. Plasma metabolite comparisons were made between participants who developed GDM (n=27) and individuals without GDM (n=23) through UPLC-MS analysis with metabolomic …
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RILIEVO DI FATTORI XENOBIOTICI, CON PARTICOLARE RIFERIMENTO A NICOTINA E COTININA, IN CAMPIONI ISTOLOGICI APPARTENENTI A FETI E NEONATI DECEDUTI PER CAUSE NATURALI.
… for toxicological substances and then by ICP-MS analysis for 61 different metals. At last, all the 151 samples have been subjected to UPLC/MS-MS technique for the relief and dosage of nicotine and cotinine.
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The determination of veterinary antibiotics in live animals and animal products
… This thesis was undertaken to develop a UPLC-MS/MS method for the simultaneous analysis of the aminoglycoside and macrolide antibiotics. This is the first time that a combination method for the two classes of compound was developed. A Waters Acquity/ Premier XE system with a dual ESI/APCI …
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Stability of heroin metabolites and oxycodone in rat hair and liver during decomposition
… and buried in controlled burial microcosms. Concentrations of oxycodone and selected metabolites or the metabolites of heroin; 6-monoacetylmorphine and morphine, incorporated within rat hair and liver were measured before and during the decomposition process. Oxycodone was analysed in …
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Advanced systems for the rapid detection of anthelmintic drugs in food
… for purpose when its performance was compared to UPLC-MS/MS analyses of milk from cows treated with benzimidazole products. In bovine liver the LOD (32 μg kg-1) and the CCβ (50 μg kg-1) were determined and the analyte recovery was in the range 77-132%. All non-compliant samples were identified …
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Metabolic Stability And Pharmacokinetics In Lead Optimization Of Potential Anti-Psychostimulant Pharmacotherapies
… it also leads to several socioeconomic problems like healthcare expenditures, lost earnings and increase in drug-related crime. The discovery and development of potential pharmacotherapies to treat cocaine dependence has been a high priority for more than two decades but still there is US-FDA …
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Improving CNS Delivery of Genistein for the Treatment of Sanfilippo Syndrome
… MPS III. Towards our goals, two major specific aims were proposed: (1) To improve accumulation of genistein in brain and (2) To evaluate the efficacy of genistein formulation. Methods: Genistein nanosuspensions were prepared by wet milling technique and characterized in order to select the most …
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Multiplatform metabolome profiling to identify specific signatures and biomarkers in blood samples: untargeted approach
… and liquid chromatography-mass spectrometry (LC-MS) was evaluated in different studies related to the field of health and disease. Thus, this doctoral thesis's main objective is to provide an objective diagnosis of disorders such as Parkinson’s, Alzheimer’s, Amyotrophic lateral sclerosis and …
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Análise toxicológica e avaliação de atividades farmacológicas de extrato hidroetanólico de cladódios de Harrisia adscendens (Gürke) Britton & Rose
… química do extrato foi analisada por LC-ESI-MS. A toxicidade aguda (2000 mg/kg) e subaguda (doses repetidas de 250, 500 e 1000 mg/kg por 28 dias) e genotoxicidade in vivo (testes do cometa e micronúcleo) foram avaliadas. Atividade antinociceptiva foi avaliada por meio do teste de contorções …
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Synthesis of analogues of nordihydroguaiaretic acid and their oxidative metabolism
… peroxidase. Results are analyzed via HPLC and UPLC-MS. It is found that 1 does not autoxidize at pH 7.4 as NDGA does. Two products are produced during incubation of 1 in rat liver microsomes with UPLC-ESI(-)-MS results giving m/z of 879.2 and 574.18. This is consistent with 1 plus 2 GSH and 1 …
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Exploitation of underused Streptomyces through a combined metabolomics-genomics workflow to enhance natural product diversity.
… of known specialised metabolites, using UPLC-MS to analyse crude extracts from growth on agar. Data was analysed using MZmine and putative metabolites were identified using freely-available MS/MS databases - primarily GNPS. This showed clear variation in production of nine identified …
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Vitamin D as a measure of health throughout the lifecycle
… Liquid Chromatography- Tandem Mass Spectrometry (UPLC-MS/MS) assay. A second aim was to examine the associations between 25OHD and a number of non-skeletal health outcomes across the lifecycle, and, thirdly and finally, to determine the dietary and supplement sources of vitamin D in two of these …
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Managing Apple Maturity and Storage to Increase the Quality of Virginia Hard Ciders
… of some individual polyphenols determined by UPLC-MS were observed in ciders made from fruit harvested at different stages. For example, cider made from optimally mature Dabinett had over 250% the concentration of procyanidin B5 that was found in cider made from fruit harvested earlier or …
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A Catharanthus roseus mutant with trace levels of secologanin and monoterpenoid indole alkaloids does not express BIS1/BIS2 transcription factors and fails to activate iridoid biosynthesis.
… roseus has not been studied. Previously, 3600 EMS (ethyl methane sulfonate) mutagenized C. roseus plants had been screened with a simple TLC (thin layer chromatography) to identify mutants with altered MIA profiles yielded one plant with trace MIA production. This trace MIA status was confirmed …
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Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… readily produced from yeast and bacterial systems using recombinant DNA technologies but require C-terminal amidation for maximum biological activity. Peptidylglycine α-amidating monooxygenase (PAM) is the only enzyme that catalyses the C-terminal amidation of peptides in vivo through its two …
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Desarrollo y evaluación toxicológica de compuestos aliáceos para su aplicación en el ámbito agroalimentario
… para la detección de OSCs en piensos, utilizando UPLC-MS/MS. Además, se realizaron estudios de biotransformación del PTSO en microsomas, revelando la formación de metabolitos no tóxicos. En el estudio toxicocinético en ratas se reveló que el PTSO no fue absorbido hacia el plasma ni tejidos …
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