Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Transposable element"”.
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Observing and quantifying transposable element activity inside living cells
Evolution is driven by the activity of transposable elements, ‘jumping genes’ that are ubiquitous to all life forms. To study their dynamics in higher resolution, transposable elements were genetically engineered for tunable expression inside living cells, then tracked in real time using …
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Measurements and analyses of transposable element activity inside living cells
Transposable elements (TEs) are DNA elements that move into different places in the DNA. Through their activity, they can restructure genomes and play crucial roles in evolution, development, and genetic disease. However, characterization of their detailed in vivo dynamics has been limited by a …
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Mechanisms governing transposable element expression in the Drosophila germ line
Transposable element activity represents a critical threat to host organisms due to its potential to damage the genome and disrupt gene expression. Transposons are known to be particularly active in the developmental context of the germ line, to fulfil their selfish drive to increase in copy number …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… cell differentiation as well as silencing of transposable elements (TEs). DNA methylation is dynamic during early mammalian development, despite being a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells …
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Revealing the transposable element dynamics driving evolutionary transitions through direct observation in live cells
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-12-01
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Analysis and application of the En transposable element and genetic study of resistance to Bipolaris maydis in maize
… molecular characterization, the En (Enhancer) transposable element has been studied extensively. It also has been used as a tool to tag and isolate genes and to elucidate mechanisms of transposition and gene regulation. The first aspect of this dissertation is on the analysis and application of …
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Identification and annotation of recombinant repeats in mammals indicates they are experimental products for creating novel transposable element families.
… of mammalian genomes are made up of repetitive elements, primarily transposable elements. Transposable elements' activities not only drive genome evolution, they contribute to the creation of novel recombinant repeats. Recombinant repeats have largely remained uncharacterized due to their …
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Whole genomic structural variant calling in soybean: Analysis on 481 different soybean lines
… discovery analysis was conducted with a focus on transposable element activities in the same 481 soybean lines. Structural variants were filtered for transposable element, and then the transposable elements identified were compared between elite soybean lines and Glycine soja lines, and then the …
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New insights into Transposable Elements from high quality genome sequences across diverse animals
… have opened the possibility of identifying new transposable element (TE) families by their polymorphic character in different haplotypes, in contrast to previous methods based on repetitiveness, homology and structural features. In this work, divided in four sections, I show how we can leverage …
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Molecular, Genetic, and Agronomic Evaluation of Wp and Wp-M in Soybean
… to result from the genetic action of a wp-m transposable element. The objectives of this research were to (1) clone a 3'5' flavonoid hydroxylase enzyme from the soybean genome and characterize its cosegregation with flower color alleles, (2) construct a molecular marker linkage map for wp , …
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Small RNAs in Tomato: from defence to development
… in plants, controlling from development to transposable element silencing and stress responses. As part of the silencing machinery, micro (mi)RNAs orchestrate silencing of their targets, either directly or through cascades of secondary small interfering (si)RNAs. To investigate the role of …
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The Evolution of Recombination Landscapes and Mechanisms in Drosophila in Light of Intragenomic Conflict
… manipulation and exploitation by selfish genetic elements. Selfish elements can increase in abundance independently of their host organism and frequently at a cost to host fitness. Several types of selfish elements act during meiosis and therefore it is possible for recombination rates and …
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Evolution of the mysRS element in the genome of rodent species
<p>Retrotransposons are a type of transposable element in which DNA copies are produced via an RNA intermediate and integrate into new chromosomal sites continually contributing to the dynamics of genomes. While sequencing the mys element among the Cricetidae family of rodents, we identified a …
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Insertion sequence IS1141: discovery, characterization, and association with Mycobacterium intracellulare colonial variation
… in O variants, it had been suggested that a transposable element might be responsible for colonial variation. The first objective was to clone the unique DNA fragment present in the 68 kb plasmid but absent from the 62 kb plasmid. The second and third objectives were to determine if the …
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Transposable Elements in Neural Progenitor Cells
… gene expression and is therefore indispensable. Transposable elements comprise about 50% of the genome and co-exist as symbionts regulated by epigenetic mechanisms - a highly defined machinery that controls gene expression and is mandatory for a proper development and maintenance of an organism. …
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Genetic Dissection of DH31 Signaling in Drosophila
… advantage of the KG09001 line because it has a transposable element inserted in the third intron of the Dh31 gene. We employed an excision screen to mobilize the element and generated 500 excision lines. Evaluation of these excision lines led to the identification of a revertant, Dh31rev, and …
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Isolation and Characterization of a Full Length Retrotransposon: CR1
<p>Transposable elements (TE) have been found in all genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes …
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Regulation of Mu transposition via communication between the transposase and Mu-encoded accessory protein
Transposable elements comprise a significant portion of both prokaryotic and eukaryotic genomes. These mobile segments of DNA have helped to shape the course of evolution by generating genetic mutations and contributing to genomic rearrangements. Transposable elements often target transposition to …
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Can plant survive without steroids: Modulation of plant architecture and sex determination through brassinosteroid biosynthesis and signaling
… mutant alleles by remobilizing a nearby Ds transposable element. Contrary to bds1 mutants, the bds2 single mutants were indistinguishable from wild-type plants. However, the bds1-R; bds2-Ds double mutants exhibited enhanced bds1 developmental defects such as plant dwarfness and tassel …
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