Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Tie2"”.
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Tumor Angiogenesis is all Tied up in Tie2-Expressing Macrophages
… resistance and further progression of disease. Tie2-expressing monocytes (TEMs) are a distinct subset of pro-angiogenic monocytes selectively recruited to breast tumors in both mouse models of cancer and breast cancer patients. Because of the hypoxic nature of tumors, we investigated if oxygen …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… hypoxic conditions. The Tie receptors, Tie1 and Tie2, are receptor tyrosine kinases that are integral to angiogenic pathways. A family of Angiopoietins, Ang1-4, have been shown to act as ligands for Tie2, of which Ang1 and Ang2 are best characterized. Activation of the receptor causes …
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Integrin-independent activation of Tie2 using the de novo designed protein H8mb
The Angiopoietin–Tie2 signaling axis is a central regulator of vascular stability, yetefforts to harness it therapeutically have been limited by the poor developability and receptor promiscuity of Angiopoietin-1 (Ang1), which binds both Tie2 and α5β1 integrin. Here we report the de novo design of a …
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Role of The Ang-Tie2 Pathway In The Invasive Recurrence of Gbm Following Anti-Vegf Therapy
… of a significant increase in the number of Tie2 expressing monocytes (TEMs) within the tumor after treatment, particularly around areas of invasion. TEMs are a pro-angiogenic subset of circulating monocytes that express the Tie2 receptor. Our data demonstrates that TEMs have higher …
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Tie-ing the endothelium: development of an mRNA-based nanotherapeutic to restore microvascular barrier function for the treatment of sepsis-induced multiple organ failure
… is a hallmark of sepsis to which the loss of Ang/Tie2 signalling, a key regulator of vascular integrity, contributes significantly, making it an attractive therapeutic target. The impairment in Tie2 pathway activity manifests most prominently in the lungs, where it drives excessive microvascular …
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The Role of Angiopoietin-2 in Signaling Through the Endothelial Receptor Tyrosine Kinase Tie1
… cells and that the extracellular domain of Tie2 is required for Ang2-mediated activation of Tie1. Furthermore, we demonstrated that Tie1 activation leads to signaling through the Akt axis, and the consequent stimulation of anti-apoptotic and pro-proliferative cellular effects. Additionally, …
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EphA4 Influences Blood Brain Barrier Disruption and Endothelial Cell Response following Traumatic Brain Injury in a Mouse Model
… studies revealed increased mRNA expression of Tie2, Ang1, and the tight junction proteins Zona Occludens and Occludin in KO mice compared to WT. As well as, connection with neuronal processes. Based off of these findings, we utilized a soluble Tie2 inhibitor to elucidate the influence of Eph …
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Therapeutic Targeting of Arteriogenesis Following Ischemic Stroke
… EphA4 restricts arteriogenesis through the Tie2 signaling axis, therefore this work sought to evaluate the endothelial cell (EC) specific role of the EphA4/Tie2 axis in acute arteriogenesis. EC-specific EphA4 KO mice displayed increased pial collateral size from 4.5 to 24-hours post-injury, …
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Unraveling the Role of EphA4 in Immune-Mediated Arteriogenesis After Ischemic Stroke
… vessel diameters. Additionally, we explored the Tie2-mediated PI3K signaling pathway in peripheral-derived monocyte/macrophages (PDM) treated with soluble Tie2-Fc and a PI3K p110α inhibitor. The results from this dissertation show that loss of PDIC-specific EphA4 led to increased collateral …
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Silencing Endothelial EphA4 Alters Transcriptional Regulation of Angiogenic Factors to Promote Vessel Recovery Following TBI
… EphA4 knockout mouse model (KO-EphA4flf/Tie2-Cre) to determine the extent to which this receptor may influence vascular regrowth following TBI. Analysis of the colocalization and proximity of endothelial and mural cell markers (i.e. PECAM-1 and PDGFRβ, respectively) in …
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Novel Immune-Regulatory Mechanisms in a Mouse Model of Traumatic Brain Injury
… We also showed that EphA4 interacts with the Tie2/Angiopoietin signaling axis to regulate macrophage phenotype on the M1/2 continuum. Overall, our work demonstrated a novel role for EphA4, mediated by Tie2, as a pro-inflammatory regulator of the peripheral-derived immune cell response to TBI.
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The Role of Age and Model Severity on Cortical Vascular Response Following Traumatic Brain Injury
… an age-at- injury dependence on the Angiopoetin-Tie2 axis, which mediates neuroprotection in a model of juvenile TBI following cortical controlled impact (CCI) that is not seen in adult mice. The protection observed was mediated, in part, by the microvascular response to CCI injury and prompted …
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Studies of Bioactive Natural Products and Mechanism-Based Bioassays
… using an assay for inhibitors of the enzyme Tie2 kinase, afforded a two sulfated cycloartanol triterpenoids. Both the major and minor compounds were identified by spectroscopic methods. Bioassay-guided fractionation of an extract of <i>Petalonyx parryi</i> yielded three known oleanane …
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The Chemokine Receptor CXCR7 Functions in Endocardial-Derived Cells to Regulate Cardiac Valve Remodeling
… cell-specific deletion of Cxcr7 using Tie2-Cre resulted in hypertrophy of the heart in adult mice because of semilunar valve stenosis, confirming important function of CXCR7 in endocardial-derived cells. This study provides valuable insight into the mechanism controlling cardiac cushion …
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Development of a novel approach to expand umbilical cord blood-derived haematopoietic stem/progenitor cells ex vivo for clinical transplantation
… analysis revealed modulation of Notch and Tie2 pathways, suggesting their potential role in improving stem cell engraftment. In addition, down-regulation of cyclin D1 and upregulation of CDK inhibitor p57 known to be important in maintenance of quiescence was observed. This work is a timely …
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Einfluss von Mistelextrakt, Selen, Kavir-Protein und Ascorbinsäure auf die Genexpression angiogener Wachstumsfaktoren und das Sprossverhalten humaner Granulosazellen
… dass GC Angiopoeitin 1, 2 und deren Rezeptor Tie2, den Vascular Endothelian Growth Factor VEGF A sowie dessen Rezeptor KDR exprimieren. Dies eröffnet die Möglichkeit eines autokrinen Loops bei GC. <br>(1) Die Stimulation mit Mistelextrakt führte bei den GC zu einer signifikanten Verminderung …
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Multiplexed angiogenic biomarker quantification on single cells
… protocols to measure cell membrane VEGFR, NRP1, Tie2, and PDGFR concentration on several cell and tissue models including human dermal fibroblasts (HDFs) in vitro, a 2D endothelial/fibroblast co-culture model in vitro, and a patient-derived xenograft (PDX) model of glioblastoma (GBM). I …
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Protein Tyrosine Phosphatase Receptor Type S (PTPRS) Regulates Hematopoietic Stem Cell Self-Renewal
… tyrosine kinases (PTK) such as c-kit, Flt-3 and Tie2. PTKs work in concert with receptor protein tyrosine phosphatases (PTPs) to maintain cellular equilibrium. The functions of PTPs in counterbalancing PTK signaling in HSCs however remain incompletely understood. Our laboratory has demonstrated …
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Eph-mediated restriction of cerebrovascular arteriogenesis
… collateral remodeling via attenuation of the Tie2/Angiopoietin-2 signaling pathway. The work presented in this dissertation demonstrate that EphA4 can be targeted therapeutically to increase pial collateral remodeling to alleviate neurological deficits after ischemic stroke.
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ELUCIDATION OF THE UNIQUE TRANSLATION OF ANGIOGENIC SIGNALING BY AORTIC VALVE CELLS
… tip cell and pericyte behaviors. Angiopoietin1-Tie2 signaling was found to regulate valve cell organization during VEC/VIC spheroid formation and invasion. Long term co-cultures demonstrated pronounced deviation of several angiogenesis and pericyte markers when measured with qRT-PCR. In the next …
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