Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"TIRF"”.
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Development of a TIRF Microscope for Single Molecule Binding Studies
… internal reflection fluorescence microscope (TIRFM) is necessary to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to …
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Super-resolution wide-field optical microscopy by use of Evanescent standing waves
… wave total internal reflection fluorescence (SW-TIRF), has been developed with resolution beyond the classical diffraction limit. The SW-TIRF approach modifies the point-spread function to effectively decrease the excitation wavelength by utilizing an evanescent standing wave, carrying high …
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High resolution imaging and lithography using interference of light and surface plasmon waves
… wave total internal reflection fluorescence (SW-TIRF), one of structured illumination techniques, with surface plasmon resonance (SPR). The SW-TIRF approach decreases the excitation wavelength by interfering two coherent light rays on the substrate and producing an evanescent standing wave field …
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Development of single molecule total internal reflection fluorescence microscope
… built a total internal reflection fluorescence (TIRF) microscope with single-molecule sensitivity to study fluorescent molecules. The microscope is modular and multifunctional with capabilities including a wide excitation wavelength range (400-2000 nm) both in objective-type and prism-type TIRF …
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Entwicklung eines fluoreszenz-optischen Evaneszenzfeldmesssystems für die Echtzeitanalyse von DNA-Mikroarrays
… der Total Internal Reflection Fluorescence (TIRF) auf die Mikroarraytechnologie übertragen, angepasst und optimiert worden, um Hybridisierungsereignisse auf DNA-Mikroarrays in Echtzeit analysieren zu können. Dazu wurden verschiedene (mikro)-optische Auflicht- und …
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Development of selective high-affinity fluorescent sensors for imaging neurotransmitters and application in cells and tissue
… of total internal reflective fluorescence (TIRF) microscopy and amperometry. A NIR fluorescent sensor NS659 was developed based on the structure of NS510. Several different synthetic routes were attempted before the sensor was successfully prepared. Upon binding to serotonin, the sensor gave …
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Probing cellular protein complexes using single-molecule pull-down
… using total internal reflection fluorescence (TIRF) microscopy. We name this technology single-molecule pull-down or SiMPull. In one reification biotinylated antibody against the protein of interest is immobilized on a flow chamber. The flow chambers are passivated using a polymer coating such …
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Regulation of Kinesin-3 activity by active zone protein SYD-2
… und Einzelmolekül Analyse mittels TIRF Mikroskopie sind bestens etablierte Methoden um die motilen Eigenschaften von Motoren wie Geschwindigkeit und Prozessivität zu testen. Wir beabsichtigen in dieser Studie ein verbessertes Verständnis der Bedeutung der SYD-2 und UNC-104 …
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Microfluidic chips for combinatorial screening applications
… Total internal reflection fluorescence (TIRF) microscopy is used for read out of the chip. Four virus-like oligomer targets with sequences corresponding to key fragments of the viruses HIV, HPV, Hepatitis A and Hepatitis B were tested against four different molecular beacons, each …
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Development of tools for single molecule peptide fluorosequencing
… total-internal reflection fluorescence (TIRF) microscopy, and Edman degradation, is a novel technology that has been developed to sequence peptides. Unique fluorophores are covalently attached to amino acid residues and through TIRF microscopy are traced through Edman degradation cycles …
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Visualizing Protease Activation, Retrochmp3 Activity, and VPR Recruitment During HIV-1 Assembly
… morphology and infectivity. Using polarization TIRF microscopy, I have directly measured protease activation and Gag cleavage. My results suggest that protease becomes active prior to recruitment of the host ESCRT proteins and scission from the cell. Chapter 3 of this thesis describes my work on …
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Anomalous diffusion of mRNA in the cytoplasm of HeLa cells
… in live cells via total internal reflection (TIRF) microscopy. In order to visualize individual RNA molecules expressing the MYH9 gene, they were labeled via MS2 stem loops bound to coat proteins tagged with the HaloTag-JF646 fluorophore. We then used single-particle tracking to obtain …
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Single-molecule imaging studies of protein dynamics
… Total Internal Reflection Fluorescence: TIRF) microscopy and imaging. The single molecule experiments of this thesis covered three broad topics. First, the adsorption mechanisms of proteins onto hydrophobic and hydrophilic fused silica surfaces were imaged and reversible and irreversible …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… internal reflection fluorescence (pol-TIRF) microscopy. The NPC is a large (~120 MDa in humans) macromolecular assembly that controls the flow of molecules into and out of the nucleus. The NPC is a key regulator of intracellular trafficking, nuclear organization, and gene expression. …
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Determinantes moleculares de la modulación de la apertura y cierre del canal de K+ hERG por dominios citoplásmicos
… de hERG, hemos desarrollado la combinación TIRF-FRET a fin de obtener datos fluorimétricos correspondientes a canales totalmente funcionales y correctamente ensamblados en la membrana plasmática. Hemos podido validar así los datos obtenidos previamente en nuestro laboratorio a partir de las …
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Serine 910 Phosphorylation of Focal Adhesion Kinase Is Critical for Costamere Assembly
… pathways. Using co-immunoprecipitation, TIRF-microscopy and FRAP, ET-1 stimulation of NRVM expressing a nonphosphorylatable, S910A-FAK mutant decreased the interaction of paxillin and vinculin within costameres. This interaction was important in stabilizing α-actinin within the protein …
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Estudio de prefactibilidad para la instalación de una planta productora de sacos de fibra natural de cabuya (Furcraea andina)
… de oportunidad (COK=10,26%), el TIRE es 66% y el TIRF es 96%.
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… by total internal reflection fluorescence (TIRF). Cy3-labeled engineered substrates containing nondegradable GFP in the prescence of ATP[gamma]S form stable pre-engaged substrate-ClpXP complexes where the whole substrate degradation pathway, from unfolding to egress of degraded products, can …
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… with total internal reflection fluorescence (TIRF) microscopy. We found that the mobility of individual enzymes was enhanced three-fold in the presence of the substrate, and the motion remained Brownian. We showed that the relative increase in diffusion is independent of the total enzyme …
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Estudio de prefactibilidad para la instalación de una planta productora de calzado de biocuero para dama con plantilla a base de canela
… respectively, while the TIRE is 34.4% and the TIRF is 150%.
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