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Showing 1 to 20 of 28 for “"TALEN"”.
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Moderne vreemde talen uit balans: een onderzoek naar behoeften aan moderne vreemde talen in relatie tot het vreemde-talenonderwijs
Contains fulltext : mmubn000001_026710846.pdf (Publisher’s version ) (Open Access)
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Bill Talen and Reverend Billy: A Shared Journey
… of the `60s fought its way into the `70s, Bill Talen began his career first as a poet, hitchhiking the interstate highways from the Midwest to the Coasts eagerly engaging the literary, intellectual, and artistic opportunities offered by those cultural venues. He settled in San Francisco where he …
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När andra ser. Gravmonument, social praktik och aktörskollektiv under 1100- och 1200-talen i Vänerlandskapet
Grave monuments showing rich imagery were made to a remarkable extent in the area around Lake Vänern during the 12th and 13th centuries. This thesis is about these monuments and about the physical remains of burials that were carried out over 800 years ago. The production of such grave monuments, …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease. Subsequent repair of …
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Probing single-molecule dynamics of molecular motors and gene editing proteins
… transcription activator-like effector nuclease (TALEN). Genome editing critically relies on selective recognition of target sites with minimal off-target effects. However, despite recent progress, the underlying search mechanism of genome editing proteins is not fully understood in the context of …
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Bedrijfsregels in verschillende vormen - Een vergelijking op toepasbaarheid tussen SWRL en Relatie algebra bij wetteksten
… een logische taal. Voorbeelden van dergelijke talen zijn relatiealgebra via de methode Ampersand en de Semantic Web Rule Language (SWRL) voor het opnemen van regels in een ontologie voor het semantisch web. In dit onderzoek wordt gekeken naar enerzijds de haalbaarheid van het implementeren van …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… include meganucleases, zinc finger nucleases, TALEN (transcription activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are approaches such as activating mutagenesis and ectopic expression, …
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Gene editing in Aedes aegypti
… repair HE-induced dsDNA breaks. TALE nucleases (TALENs) are a group of artificial enzymes capable of generating site-specific DNA lesions. To examine the ability of TALENs for gene editing in Ae. aegypti, a pair of TALENs targeted to the kmo gene were expressed from a plasmid following embryonic …
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Mellan fromhet och vidskepelse: Materialitet och religiositet i det efterreformatoriska Sverige
… materiella i fromhetslivet under 1600- och 1700-talen. Avhandlingens första del behandlar kyrkorummet och dess föremål, som sidoaltare, bilder och reliker. Den andra delen utforskar sakralt laddade punkter i landskapet, som heliga källor och fristående kors, och hur den lokala topografin kunde …
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Mammalian genome engineering: Fundamental investigation and method development
… have been adapted for use in mammalian systems. TALEN (Transcription Activator-Like Effector Nucleases) and CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems are the most widely employed gene-editing tools presently. The modularity and specificity of these …
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Mother Rind
<p>This collection of poems and hybrid lyric essays explores the links between ancestral history and the natural world. As femmes and woman, much of our history had been lost in violence and war. Much of it is undocumented, untranslated, footnoted. However, the natural world, the land and plants of …
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Discovery of New Regulatory Proteins and Mechanisms in Muscle Biology and Disease
… LMOD3 in NM, we generated Lmod3 knockout mice by TALEN-mediated mutagenesis. Preliminary data shows that loss of Lmod3 results in a degenerative skeletal muscle myopathy. Thus, we propose that loss of Klhl40 directly results in decreased Neb and Lmod3 causing thin filament disruption and …
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Genetic Correction of Duchenne Muscular Dystrophy using Engineered Nucleases
… Transcription activator-like effector nucleases (TALENs) were engineered to mediate highly efficient gene editing after introducing a single TALEN pair targeted to exon 51 of the dystrophin gene. This led to restoration of dystrophin protein expression in cells from DMD patients, including …
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Modeling Cancer Using Li-Fraumeni Syndrome Patient-Derived Induced Pluripotent Stem Cells
… (Y205C) in LFS patient derived iPSCs using TALEN-mediated precise gene editing. Similar approach was used to generate two H1 human embryonic stem cells (hESCs) carrying homozygous <em>TP53 </em>R282W and <em>TP53</em> R248W mutation. These engineered iPSCs/hESCs offers exciting opportunities …
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Investigating Mechanisms of Telomere End-Protection
… targeting in human cells, we constructed TALEN-mediated knockouts of human Rap1 in numerous cell lines. Loss of human Rap1 did not lead to an induction of NHEJ, or show consistent changes in telomere length, indicating that similar to mouse Rap1, human Rap1 does not have an important …
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The role of transcription factor GATA6 in the development of the human pancreas
… The use of site-specific nucleases such as TALENs for such a purpose represents a paradigm shift in disease modelling, where TALENs are capable of directly correcting disease-causing mutations, therefore permanently eliminating the symptoms with precise genome modifications. Alternatively, …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… SV40 used to target the TAL effector nuclease (TALEN) to the nucleus worked poorly in P. sojae. In the first part of this work (Chapter 2), I used confocal microscopy to identify features of nuclear localization in oomycetes that differ from animals, plants and fungi, based on characterization …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… the ER, respectively. Additionally, by using TALEN technology, I edited the sedlin gene in medaka to create a SEDLIN KO stable line (Sedl-/-). Sedl-/- fish have severe skeletal defects, which are similar to those observed in human SEDT patients, and die soon after birth suggesting that sedlin …
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Molecular mechanisms of mammalian cell survival and differentiation
… of generating XPLN knockout mice using the TALEN technology, in order to facilitate future in vivo studies of XPLN. In Chapter 4, I identified and characterized microRNA-146b (miR-146b) as a novel positive regulator of skeletal myogenesis. Inhibition of miR-146b led to reduced myoblast …
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