Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"TALEN"”.
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Moderne vreemde talen uit balans: een onderzoek naar behoeften aan moderne vreemde talen in relatie tot het vreemde-talenonderwijs
Contains fulltext : mmubn000001_026710846.pdf (Publisher’s version ) (Open Access)
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease. Subsequent repair of …
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Probing single-molecule dynamics of molecular motors and gene editing proteins
… transcription activator-like effector nuclease (TALEN). Genome editing critically relies on selective recognition of target sites with minimal off-target effects. However, despite recent progress, the underlying search mechanism of genome editing proteins is not fully understood in the context of …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… include meganucleases, zinc finger nucleases, TALEN (transcription activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are approaches such as activating mutagenesis and ectopic expression, …
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Gene editing in Aedes aegypti
… repair HE-induced dsDNA breaks. TALE nucleases (TALENs) are a group of artificial enzymes capable of generating site-specific DNA lesions. To examine the ability of TALENs for gene editing in Ae. aegypti, a pair of TALENs targeted to the kmo gene were expressed from a plasmid following embryonic …
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Mammalian genome engineering: Fundamental investigation and method development
… have been adapted for use in mammalian systems. TALEN (Transcription Activator-Like Effector Nucleases) and CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems are the most widely employed gene-editing tools presently. The modularity and specificity of these …
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Mother Rind
<p>This collection of poems and hybrid lyric essays explores the links between ancestral history and the natural world. As femmes and woman, much of our history had been lost in violence and war. Much of it is undocumented, untranslated, footnoted. However, the natural world, the land and plants of …
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Discovery of New Regulatory Proteins and Mechanisms in Muscle Biology and Disease
… LMOD3 in NM, we generated Lmod3 knockout mice by TALEN-mediated mutagenesis. Preliminary data shows that loss of Lmod3 results in a degenerative skeletal muscle myopathy. Thus, we propose that loss of Klhl40 directly results in decreased Neb and Lmod3 causing thin filament disruption and …
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Genetic Correction of Duchenne Muscular Dystrophy using Engineered Nucleases
… Transcription activator-like effector nucleases (TALENs) were engineered to mediate highly efficient gene editing after introducing a single TALEN pair targeted to exon 51 of the dystrophin gene. This led to restoration of dystrophin protein expression in cells from DMD patients, including …
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Modeling Cancer Using Li-Fraumeni Syndrome Patient-Derived Induced Pluripotent Stem Cells
… (Y205C) in LFS patient derived iPSCs using TALEN-mediated precise gene editing. Similar approach was used to generate two H1 human embryonic stem cells (hESCs) carrying homozygous <em>TP53 </em>R282W and <em>TP53</em> R248W mutation. These engineered iPSCs/hESCs offers exciting opportunities …
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Investigating Mechanisms of Telomere End-Protection
… targeting in human cells, we constructed TALEN-mediated knockouts of human Rap1 in numerous cell lines. Loss of human Rap1 did not lead to an induction of NHEJ, or show consistent changes in telomere length, indicating that similar to mouse Rap1, human Rap1 does not have an important …
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The role of transcription factor GATA6 in the development of the human pancreas
… The use of site-specific nucleases such as TALENs for such a purpose represents a paradigm shift in disease modelling, where TALENs are capable of directly correcting disease-causing mutations, therefore permanently eliminating the symptoms with precise genome modifications. Alternatively, …
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Nuclear Localization of Proteins and Genome Editing in the Oomycete Phytophthora sojae
… SV40 used to target the TAL effector nuclease (TALEN) to the nucleus worked poorly in P. sojae. In the first part of this work (Chapter 2), I used confocal microscopy to identify features of nuclear localization in oomycetes that differ from animals, plants and fungi, based on characterization …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… the ER, respectively. Additionally, by using TALEN technology, I edited the sedlin gene in medaka to create a SEDLIN KO stable line (Sedl-/-). Sedl-/- fish have severe skeletal defects, which are similar to those observed in human SEDT patients, and die soon after birth suggesting that sedlin …
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Molecular mechanisms of mammalian cell survival and differentiation
… of generating XPLN knockout mice using the TALEN technology, in order to facilitate future in vivo studies of XPLN. In Chapter 4, I identified and characterized microRNA-146b (miR-146b) as a novel positive regulator of skeletal myogenesis. Inhibition of miR-146b led to reduced myoblast …
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Single molecule investigation of transcription activator-like effector search dynamics
… when fused to nuclease domains, thereby yielding TALEN systems. Despite pervasive use of TALENs for editing crops, small animals, eukaryotic stem cells, and human T-cells, remarkably little is known about the molecular mechanisms used to locate and bind their DNA target sites. This work describes …
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Creation and characterization of mice with a mutation disrupting binding of a transcriptional repressor of insulin-like growth factor 2
… transcription activator-like effector nucleases (TALEN) with co-injection of oligo donors. Two founders were generated containing mutations that disrupted the ZBED6 binding site. One founder mouse contained the targeted G>A point mutation that mimicked the mutation in pigs, and the other founder …
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Genomic Analysis of Human Spinal Deformity and Characterization of a Zebrafish Disease Model
… responsible for the skolios phenotype, we used TALENs to create additional mutant alleles. We isolated three new TALEN-induced mutations that caused frameshift mutations in kif6. All zebrafish homozygous or compound heterozygous for kif6 frameshift mutations developed body axis curvature that …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… zinc finger nucleases (ZFN) or TALE-nucleases (TALEN), respectively. By targeting these pairs of engineered nucleases adjacent to a site of interest, a DNA double-strand break (DSB) can be produced, leading to degradation of the mtDNA molecule. Using novel architecture to express and localise …
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