Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 50 for “"Small Gtpases"”.
-
A New Approach To Identifying Effectors and Regulators of Small GTPases
… endomembrane system. It is widely accepted that small GTPases of the Ras superfamily are major regulators of membrane trafficking and signalling, ensuring specificity and efficiency through their spatiotemporal regulation.Identifying the proteins interacting with small GTPases is vital for …
-
DHHC PROTEIN ACYLTRANSFERASES CATALYZES THE LYSINE PALMITOYLATION OF MEMBERS OF THE RAS SUPERFAMILY OF SMALL GTPASES
… labeling the lysine residues of multiple GTPases. This acylation event is confirmed as lysine fatty acylation through the use of the SIRT2 in-vitro defatty-acylation assay and mutagenesis of the modification sites and P-32 NAD + assay. Knockdown of the zDHHC7 gene was able to perturb the …
-
UNDERSTANDING THE RAL GTPASES: THE REGULATION BY PROTEIN LYSINE FATTY ACYLATION, SIRT2, AND INTERACTING PROTEINS
The RAS superfamily of small GTPases are molecular switches that control a wide range of cellular processes. Detailed understanding of these small GTPases is important for elucidating cell signaling mechanisms and identifying new disease treatment strategies. My thesis is centered on understanding …
-
Ras Intrinsic and Extrinsic Pathways in Human Cancer
… composed of H, N, and KRas, function as small GTPases that act as "molecular switches" relaying signals from the cell membrane to the rest of the cell in a highly regulated manner. However, Ras signaling is aberrantly activated in a majority of human cancers either through an activating …
-
Developmental and Cellular Functions of Delta-Catenin
… compartments and modulates cadherin stability, small GTPase activities and gene transcription. In mammals, delta-catenin exhibits neural specific expression, with its knock-out in mice correspondingly producing cognitive defects and synaptic dysfunctions.</p> <p>My work instead employed the …
-
A Structural Basis for Host Cytoskeletal Disruption and Virulence by Yersinia Protein Kinase A
… pathways, often targeting the Rho family of small GTPases which regulate actin cytoskeletal dynamics. A critical virulence determinant in Yersinia species is the Yersinia protein kinase A, or YpkA, a multi-domain protein that disrupts the eukaryotic actin cytoskeleton. YpkA contains a Ser/Thr …
-
Structural and biochemical insight into the interactions of Cdc42 with TOCA1 and N-WASP
Cdc42 is a member of the Rho family of small GTPases, which, together with its homologues RhoA and Rac1, controls a multitude of cellular functions via the actin cytoskeleton. Cdc42 exerts its effects on the cytoskeleton via effector proteins of the Wiskott-Aldrich Syndrome (WASP) family and the …
-
Structural Characterisation of the Mammalian SRP Receptor
… In all three kingdoms of life the conserved GTPases in SRP and SR (in eukaryotes SRP54 and SRα, respectively) enable the formation of the docking complex in a GTP dependent manner. SRβ is the third and least understood GTPase participating in cotranslational targeting in eukaryotes. Therefore …
-
The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
… We show that Vps54 may be interacting with small GTPases Rab7 and Rab11 at different life stages to further regulate motor neuron development and function. Taken together, these data suggest that Vps54 plays a major role in the development and functional regulation of motor neurons, while …
-
Towards Inhibition of Oncogenic RhoA Signaling using Stapled Peptides
… or difficulty in developing traditional small molecule inhibitors to many of the identified oncogenic targets, including the Ras superfamily of small GTPases. Peptide therapeutics are emerging as promising new modalities to more efficiently inhibit these "undruggable" targets. RhoA is a …
-
Determining the differential roles of the dock family of GEFs in drosophila development
… Dock-ELMO complexes activate the Rho family of small GTPases to mediate a diverse array of biological processes, including cell motility, apoptotic cell clearance, and axon guidance. Overlapping expression patterns and functional redundancy among the eleven vertebrate Dock family members, which …
-
Structural and Biochemical Characterisation of the HR1 domains of the Protein Kinase C-related kinase family
The Rho family of small GTPases act as molecular switches in the cell and are involved in signalling pathways implicated in cytoskeletal organisation. To mediate their effects, they interact with downstream effectors. The protein kinase C-related kinase (PRK) family of serine/threonine kinases …
-
Control of Neuronal Circuit Assembly by Gtpase Regulators
… the regulation of cytoskeletal dynamics are the small GTPases of the Rho family. Small RhoGTPases are regulated by GTPase exchange factors (GEF) and GTPase activating proteins (GAP), which can switch GTPases into "on or off" states, respectively. It is thought that GEFs and GAPs function as …
-
The disordered N-terminus of RLIP76: a hub for protein and membrane interactions
RLIP76 is an effector for the Ral family of small GTPases. Ral proteins are themselves downstream of Ras, and Ras mutations drive around 20% of all cancers. RLIP76 has been implicated in a number of cell processes ranging from receptor mediated endocytosis, to mitochondrial fission and metabolite …
-
Rab5 Activation by the Vps9 Domain
… of the early stages the endocytic pathway, the small GTPases of the Rab5 family serve an essential function in integrating intracellular protein traffic and these cell signaling events. Rab5 proteins exert their influence on protein trafficking only when bound to GTP. A large family of proteins …
-
RalGDS-Dependent Cardiomyocyte Autophagy Is Necessary for Load-Induced Ventricular Hypertrophy
… unknown. Here, I set out to explore the role of small G protein-dependent membrane trafficking in stress-induced cardiomyocyte remodeling and autophagy. To explore underlying mechanisms, I tested in cultured neonatal cardiomyocytes two isoforms of Ral that are downstream of RalGDS (RalA, RalB) …
-
Rab5 Activation by the Vps9 Domain
… of the early stages the endocytic pathway, the small GTPases of the Rab5 family serve an essential function in integrating intracellular protein traffic and these cell signaling events. Rab5 proteins exert their influence on protein trafficking only when bound to GTP. A large family of proteins …
-
P120-Catenin Regulates Rest and Corest, and Modulates Mouse Embryonic Stem Cell Differentiation
… and functional interactions with cadherins, small-GTPases and transcriptional regulators. We report here that p120-catenin binds to and negatively regulates REST and CoREST, that others have indicated form a repressive complex having diverse key roles in developmental and pathologic gene …
-
Rasa3 controls turnover of endothelial cell adhesion and vascular lumen integrity by a Rap1-dependent mechanism
Rasa3 est une protéine activatrice des small GTPases et fait partie de la famille GAP1 qui cible Ras et Rap1. Bien que l’inactivation catalytique ou la délétion de Rasa3 en souris résulte en de nombreuses hémorragies et à la létalité embryonnaire, la fonction biologique sous-jacente à ces défauts …
-
Regulation of Blood Vessel Development via Rho Family GTPase Signaling
The Rho family of small GTPases has been shown to be required in endothelial cells (ECs) during blood vessel formation. However, the underlying cellular events controlled by different GTPases remain unclear. Here, we assess the cellular mechanisms by which Rho family GTPase proteins and their …
Page 1 of 3