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Showing 1 to 20 of 438 for “"Sample preparation"”.
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Investigation of monolithic materials for protein sample preparation
… and the understanding of biological processes. Sample preparation is a bottleneck in systems for chemical analysis and it is a required step in proteomics in order to remove interferences and preconcentrate the proteins. In addition, protein reduction and alkylation before digestion is a …
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Sample preparation methodologies for MALDI-MS imaging and related topics.
… explored in this thesis with an emphasis on the sample preparation procedure and method development for small molecule analysis for a range of samples. The two main themes that have been focussed on are the pharmaceutical and metabolomic applications of this state of the art technique.MALDI MSI …
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
Biological assays for various biological samples are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific …
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Sessile Droplet Microfluidic Platforms for Improvements in Mass Spectrometry Sample Preparation
… devices while maintaining their advantages (low sample consumption, high throughput, and easy automation and portability). Previous work by Bachus et al. has shown that SDMF arrays can be made rapidly and reproducibly by applying a hydrophobic coating to a glass surface, then milling surface …
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Versatile, automated sample preparation and detection of contaminants and biological materials
… To be deployable outside a laboratory, sample preparation needed to be automated. Automation was achieved by combining the LabReader with the already developed LabTube, a disposable platform for automated DNA extraction inside a standard centrifuge. Performing DNA amplification/readout …
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Novel customisable phases for micro solid-phase extraction and automated biological sample preparation
… specificity. However, major challenges lie with sample preparation involving lengthy workflows, limited automation, and challenges related to highly complex biological samples where several techniques are applied. This thesis aims to improve sample preparation techniques to provide an accurate, …
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Optimization on Single-Pot, Solid-Phase-Enhanced Sample Preparation Method for Proteomics Workflow
… proteomics relies on effective sample preparation to achieve accurate protein identification and quantification. Among current methods, Single-Pot, Solid-Phase-Enhanced Sample Preparation (SP3) is widely used because of its simple workflow, compatibility with different buffer …
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Comparing Extraction Methods in Sample Preparation for the Quantification of Cannabinoids in Industrial Hemp
… does not, however, specify standard methods for sample preparation or quantification of cannabinoids (including THC) in Cannabis. Extraction efficiency of phytochemicals is well-known to depend on the solvent and extraction method used. In this project, we evaluated the effect of sample …
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
<p>Sample preparation is vital in nucleic acid analysis. Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false …
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Optimization of sample preparation in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry of macromolecules
… was achieved by investigating the influence of sample preparation variables such as the choice of solvent and choice of matrix-analyte ratio. It was demonstrated that the use of ethyl acetate as a solvent and 2,5-Dihydroxybenzoic acid as a matrix yielded the highest signal intensity for the pure …
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Supercritical fluid extraction as a sample preparation technique for the isolation of polar pharmaceutical compounds
… from leaves. Each SFE method reduced the time, sample handling, costs, and liquid solvents needed as compared to the conventional liquid extraction technique. While achieving comparable recoveries, reproducibilities were much improved for the two SFE methods with RSD's of 3.8 and 5.2%, …
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Sample preparation of membrane proteins suitable for solid-state MAS NMR and development of assignment strategies
… determination of non-crystalline solid protein preparations has been made possible through rapid progress of solid-state MAS NMR methodology for biological systems. Castellani et. al. solved and refined the first structure of a microcrystalline protein using only solid-state MAS NMR …
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DEVELOPMENT AND APPLICATION OF MINIATURIZED SAMPLE PREPARATION TECHNIQUES COMBINED WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY IN ENVIRONMENTAL ANALYSIS
… the development and application of miniaturized sample preparation techniques for environmental analysis. For sorbent-based microextraction, the applicability and feasibility of graphene or modified graphene as sorbent was investigated and evaluated. The possibility of graphene serving as sorbent …
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Enhancing the science return of Mars missions via sample preparation, robotic surface exploration and in orbit fuel production
… probes will most likely be equipped with complex Sample Preparation And Transfer (SPAT) facilities. Future rovers will need to be able to perform longer traverses and delicate sample acquisition operations. Mars return missions would benefit from a new propulsion system, with better fuel and …
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Sample Preparation/Concentration for Trace Analysis in GC/MS (A study of solid phase microextraction and headspace sampling)
… Veltol Plus® and short chain fatty acids in food samples were analyzed by GC/MS. Since SPME is not directly applicable to solid samples, SPME associated with MAE techniques was studied for solids, primarily food samples. The efficiency of classical solvent extraction and MAE was compared. The …
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Sample preparation methods and molecular based detection for the rapid isolation and identification of Listeria monocytogenes in food samples
Listeria monocytogenes is a Gram-positive bacterium responsible for listeriosis, a food-borne disease, which may result in severe illness and possible death. The importance of L. monocytogenes as a food-borne pathogen has been recognized since the 1980's when a correlation between the cunsumption …
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Optimisation of sample preparation for DNA extraction from formalin fixed paraffin embedded tissues of unresolved sudden unexpected death cases
… in the effectiveness of the technique for aged samples. The DNA extraction method developed in this study yielded a median of 320 ng (287 ng - 698 ng) of DNA with 55 % of DNA fragments being at least 400 bp in size. These results are especially informative for downstream molecular analyses, …
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