Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"SNARE complex"”.
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Analysis of Synaptotagmin-SNARE Complex Interactions by One-Dimensional NMR Spectroscopy
… numerous proteins at the neuronal synapse. The SNARE proteins form a complex that mediates fusion between the synaptic vesicle and plasma membrane. The protein synaptotagmin-1 is the major sensor for the calcium concentration. Synaptotagmin-1 and the SNARE complex are therefore believed to …
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Biophysical Characterization of SNARE Complex Disassembly Catalyzed by NSF and alphaSNAP
… sind Vertreter der konservierten Familie der SNARE-Proteine von essentieller Bedeutung. Man vermutet, dass gerichtete Komplexbildung zwischen SNAREs auf gegenueberliegenden Membranen dazu fuehrt, dass die zu fusionierenden Mebranen in raeumliche Naehe gezogen werden und schliesslich …
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Competition Between Synaptotagmin 1 and Complexin for SNARE Complex Binding, Controls Fast Synaptic Vesicle Exocytosis
… vesicles that were primed for release by SNARE complex assembly. Besides synaptotagmin 1, fast Ca2+- triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their functions are coupled. To clarify previous …
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Structural Studies of Complexin/SNARE Interactions
… was to delineate interactions between complexin and SNARE components that lead to the formation of a primed minimal fusion machinery. We have generated and used new constructs of short forms of synaptobrevin and complexin, as well as constructs of SNAP-25 and syntaxin that have …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… plasma membrane. Formation of a highly stable complex, known as the SNARE (soluble NSF-attachment protein receptors) complex, brings the two membranes close in space. SNARE complex formation is required but probably not sufficient for fusion to occur. An increase in the local Ca2+ concentration …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… plasma membrane. Formation of a highly stable complex, known as the SNARE (soluble NSF-attachment protein receptors) complex, brings the two membranes close in space. SNARE complex formation is required but probably not sufficient for fusion to occur. An increase in the local Ca2+ concentration …
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… factor attachment protein receptor (SNARE) complex formation and movement into membrane rafts in preparation for the acrosome reaction. Two t-SNAREs and a v-SNARE form a trans-SNARE complex, which is required for exocytosis. The core complex along with other regulatory proteins exerts …
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Dissection of the Roles of Synaptotagmins in Calcium Dependent Neurotransmitter Release
… domain is also involved in interactions with the SNARE complex, which is the central component of neuronal membrane fusion machinery. Clarifying the molecular details of these interactions is crucial for understanding how Syt1 cooperates with SNARE complex to trigger calcium-dependent membrane …
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Mechanical Properties of Individual Molecules : An Interface between the Structure and the Function of the Molecules
… to the study of mechanical properties of the SNARE-complex. The SNARE-complex consists of three proteins: syntaxin, synaptobrevin and SNAP-25. We measured and analyzed the mechanical properties of the interactions between four combinations between the building blocks of the SNARE-complex. …
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Structure and Function of Proteins Involved in Regulated Secretion: Synaptotagmins and Complexin
… speed with the precise timing necessary for complex functions such as sight, motor control, and conscious thought. This study will explore the role of three such regulators through biophysical and structural methods. There are a number of proteins that are essential for membrane fusion. The …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
… In one project, I investigated whether trans-SNARE complexes can be disassembled by NSF-αSNAP. I showed that trans-SNARE complex formation in the presence of NSF-αSNAP requires both Munc18-1 and Munc13-1, and is facilitated by synaptotagmin-1. I proposed a model whereby Munc18-1 and Munc13-1 …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… 1 and SNAP-25, which are collectively known as SNAREs and form a tight complex (the core complex). The assembly of the core complex may mediate membrane fusion. Complexin is a highly conserved cytoplasmic protein that binds tightly to the SNARE complex. Analysis of the interaction between …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… The release machinery includes three SNAREs (soluble N-ethylmaleimide sensitive factor adaptor protein receptor) and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that …
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Expression and Function of Complexin 1 in Mammalian Sperm Acrosomal Exocytosis
… exocytosis. Exocytosis is regulated by the SNARE complex, whose core includes three proteins. Complexins 1 and 2 are highly related cytosolic proteins that bind tightly to the assembled SNARE complex and promote neuronal exocytosis. Studies of complexin 1 and 2 deficient mice suggest that …
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N-ethylmaleimide sensitive factor (NSF) is a regulator of plasma membrane rupture and necrotic cell death
… screen was performed. We identified SNARE complex members, mediators of canonical membrane fusion events, as necessary for Ca2+ and ROS-induced plasma membrane rupture andnecrosis. We targeted N-ethylmaleimide sensitive factor (NSF) due to its requirement in SNARE recycling, lack of …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… The central fusion machinery includes the SNARE proteins synaptobrevin, syntaxin and SNAP-25, as well as Munc18. Besides these universal components, many other neuronal specific proteins are also involved in regulating Ca2+-triggered release. Interestingly, most of these regulatory proteins …
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The Functional Organization of Synaptic Vesicle Pools In A Retinal Bipolar Neuron
… vesicle fusion at these synapses is the role of SNARE (Soluble N-ethylmaleimide sensitive factor Attachment Protein Receptor) proteins. Vesicles at conventional neurons undergo SNARE complex-mediated fusion. However a recent study has suggested that ribbon synapses involved in hearing can operate …
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Structural and Functional Studies of Munc18 and SNARE Proteins
… are proteins from the Sec1/Munc18 (SM) and SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) families. SNAREs function by forming a four-helix bundle called SNARE complex; assembly of the SNARE complex brings two membranes together and is key for membrane fusion. …
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Reconstitution of Munc18-Dependent Mast Cell Degranulation
… fusion machinery. These proteins, called SNAREs are regulated by the Sec1/Munc18 (SM) protein family (Axle Lorentz, Baumann, Vitte, & Blank, 2012). Mast cells express three mammalian Munc18 isoforms (a, b, and c), which are linked to SNARE-dependent exocytosis in numerous organisms. …
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Regulation of membrane fusion by Tlg2p & Vps45p through the endosomal system of Saccharomyces cerevisiae
SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. SNARE proteins are subject to multiple levels of regulation, one of which is imbedded in the syntaxin (Qa-SNARE) molecule. It has been demonstrated that some syntaxins adopt a closed …
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