Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"SAHA"”.
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Therapeutic potential of the anti-fibrotic drug Suberanilohydroxamic acid (SAHA) in canine corneal fibrosis
… mechanisms mediating the antifibrotic effect of SAHA in the canine cornea using an in vitro model and (2) to develop a novel in vivo model of corneal fibrosis in dogs utilizing alkali burn and assess the ability of SAHA to inhibit fibrosis using this canine model. Methods: In study 1, canine …
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The structural requirements of histone deacetylase (hdac) inhibitors: suberoylanilide hydroxamic acid (saha) analogues modified at c3, c6, and c7 positions enhance selectivity
… approved drug suberoylanilide hydroxamic acid (SAHA, Vorinostat), have cleared clinical trials and emerged as anti-cancer drugs. However, SAHA inhibits all of the 11 metal ion-dependent HDAC proteins. Therefore, we synthesized several libraries of small molecule HDAC inhibitors based on SAHA to …
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Development Of Elisa-Based Hdac Activity Assay For Identification Of Isoform Selective Hdac Inhibitors
… are in various stages of clinical trials. SAHA and Romidepsin are FDA-approved drugs for the treatment of cutaneous T-cell lymphoma. Human HDAC proteins have high sequence similarity and hence many known inhibitors non-specifically interact with all or most of the eleven HDAC isoforms, …
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Defining The Role of Egfr Acetylation In Cellular Processes: Clinical Implications
… observed that suberoylanilide hydroxamic acid (SAHA, a deacetylase inhibitor) enhanced TKI-induced cancer cell death, which further led us to question whether SAHA-mediated sensitization to TKI was associated with EGFR acetylation. What we know so far is that SAHA can inhibit class I and II …
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Histone deacetylase inhibitors as novel neuroprotective agents in in vitro and in vivo models of Parkinson’s disease
… of the HDAC-Is, suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) were investigated in in vitro and in vivo models of PD. Neither inhibitors protected dopaminergic cell lines, SH-SY5Y and N1E-115, against hydrogen-peroxide (H2O2) and MPP+-induced toxicity. Except, at the highest …
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Histone Deacetylase 1: Mutagenesis And Small Molecule Studies
… on testing the active E98A mutant against SAHA inhibitor analogs present in our lab. C-2, C-3 and N-SAHA analogs were screened using a plate based ELISA assay which we developed and validated. All SAHA analogs screened did not qualify for a HDAC1-bump inhibitor system.</p>
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Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors
… the inhibitor suberoyl anilide hydroxamic acid (SAHA) has been FDA approved for treatment of cutaneous T-Cell lymphoma. Currently, most of the known HDAC inhibitors are non-selective, which causes non-specific binding to the active sites of all HDAC isoforms, including those not involved in …
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Novel Micropatterned Substrate for Investigation of Ovarian Cancer Aggregate Formation and Response to Epigenetic Drug Therapy
… drugs such as suberoylanilide hydroxamic acid (SAHA). The micropatterned substrate supported cell proliferation, migration, and aggregate formation while maintaining phenotype and viability. Treating aggregates with SAHA maintained cell viability but caused disaggregation of SKOV3 aggregates. …
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Identification of Cell Biomechanical Signatures Using Three Dimensional Isotropic Microstructures
… cells with experimental breast cancer drug, SAHA, on the second generation of substrates, significantly altered the cells morphology, cytoarchitecture and adhesion pattern inside the 3-D microstructures. Third generation of silicon substrates was developed for comprehensive analysis on …
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Characterisation of an acetylation-dependent FLIP/Ku70 complex that regulates FLIP expression
… system. Moreover, degradation of c-FLIP by SAHA induced caspase 8-dependent apoptosis that was also dependent on the expression of death receptors, particularly DR5. In addition, it was found that SAHA significantly down-regulated c-FLIP expression and retarded the growth of colorectal …
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Enhancement of death receptor-mediated apoptosis in multiple myeloma cells
… whether the Histone Deacetylase (HDAC) inhibitor SAHA, or inhibitors of the histone methyltransferases G9a and EZH2 (BIX 01294 and GSK343) and Nuclear export inhibitor (NEI) LMB, enhance TRAIL-induced apoptosis and overcome TRAIL resistance in both suspension culture, and 3D cell culture as a …
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Anti-Tumor Effects of The Notch Pathway In Gastrointestinal Stromal Tumors
… the FDA approved histone deacetylase inhibitor SAHA (Vorinostat) caused dose-dependent upregulation of <em>Notch1</em> expression and a parallel decrease in viability in these cells. Retroviral silencing of downstream targets of Notch with dominant negative <em>Hes-1 </em>as well as …
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A Microfabricated Bioimpedance Sensor with Enhanced Sensitivity for Early Breast Cancer Detection
… and fabricated. Suberoylanilide hydroxamic acid (SAHA), an FDA-approved anti-cancer agent was used to improve the sensitivity of the bioimpedance sensor towards cancer cells by selectively modifying their cytoarchitecture.
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EFFECTS OF HISTONE DEACETYLASE INHIBITORS ON ENERGY METABOLISM
… class selective HDAC inhibitors (HDACi), such as SAHA(pan-inhibitor), MS275 (Class I HDAC inhibitor) and MC1568 (Class II HDAC inhibitor), transcriptome analysis revealed an increase of OXPHOS genes and of genes encoding fatty acid catabolic enzymes, following treatment with pan or class I HDACi. …
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Genomic Analysis of Chemo-Resistance to HDAC inhibitor in Gastric Cancer cells
… with multiple HDACi compounds (trichostatin A, SAHA and MS275), we identified two distinct classes of lines exhibiting either HDACi sensitivity or resistance. Genomic comparisons between the sensitive and resistant classes using two independent microarray platforms identified RNH1, encoding a …
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Caratterizzazione mediante HPLC-MS di modifiche post-trasduzionali di proteine istoniche
… state trattate con due inibitori più potenti, SAHA e MS275, alla stessa concentrazione. In entrambi i casi il metodo messo a punto ha permesso di evidenziare l’aumento dei livelli di acetilazione indotto dal trattamento con gli inibitori; ha inoltre messo in luce differenti livelli di …
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Nuclear receptor co-repressor functions in prostate cancer; in vitro, in vivo and in silico approaches
… inhibition of NCOR1, via the HDAC inhibitor SAHA, or NCOR1 knock-down, via shRNA, restored PC-3 cells sensitivity to NR agonists with exception of Vitamin D and Thyroid Hormone T3. NCOR1-knock down led also to a re-expression of basally repressed genes, as measured via Microfluidic Gene-Card …
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Nuclear receptor co-repressor actions in bladder cancer
… inhibitor Suberoylanilide hydroxamic acid (SAHA), resulting in strongly additive anti-proliferative responses to FXR, VDR and PPAR-γ ligands in NCoR1 over-expressing cells; confirmed as a G1/S phase cell cycle arrest in EJ-28. Microarray profiling revealed unique regulation of genes involved …
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Can changes in SR protein acetylation affect alternative splicing?
… inhibition, using the HDAC inhibitors MS-275 and SAHA. RNA from treated cells was isolated for use with RT-PCR arrays developed by Peter Stoilov. Western blotting was used to determine efficacy of HDAC inhibitors on total cellular acetylation. The future goal is to understand how acetylation …
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The Use of the CAfFEINE Framework in a Step-by-Step Assembly Guide
… The CAfFEINE Framework, which was created by Dr. Saha, is a context-aware affective feedback loop in an intelligent environment. For the research described in this thesis, the focus will be on analyzing a user's physiological response to the service provided by an intelligent environment. To test …
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